Alkaline protease production by submerged fermentation in stirred tank reactor using Bacillus licheniformis NCIM-2042: Effect of aeration and agitation regimes

Potumarthi, Ravichandra; Ch., Subhakar; Jetty, Annapurna

doi:10.1016/j.bej.2006.12.003

Cited by 109 publications

(59 citation statements)

References 28 publications

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“…The production of extracellular protease in microorganisms is influenced significantly by physical factors such as pH, temperature, dissolved oxygen, incubation time, inoculum level, aeration and agitation and medium constituents (Hanlon et al, 1982;McKeller and Cholette, 1984;Kole et al, 1988;Calik et al, 2002Calik et al, , 2003Shafee et al, 2006;Potumarthi et al, 2007). Response surface methodology (RSM) is a collection of statistical techniques for designing experiments, building models, evaluating the effects of factors and searching optimum levels of parameters for desirable responses (Myers and Montgomery, 2002).…”

Section: Introductionmentioning

confidence: 98%

Effect of physical parameters, carbon and nitrogen sources on the production of alkaline protease from a newly isolatedBacillus pseudofirmus SVB1

2009

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-The effect of process physical parameters (initial pH of the medium, temperature and rpm of the shaking incubator) on the production of alkaline protease from a newly isolated Bacillus pseudofirmus SVB1 was studied using central composite design technique. The individual optimum levels of initial pH of the medium, temperature and rpm of the shaking incubator were found to be 9.2, 27.3 °C and 195, respectively for the production of alkaline protease (specific activity, U/mg of protein) and 11, 28.9 °C and 210, respectively for cell growth. By applying multiresponse analysis method of generalized distance approach for production and growth, the optimal levels were found to be 9.9, 28.1 °C and 202, for initial pH of the medium, temperature and rpm of the shaking incubator, respectively. After optimization, the production of alkaline protease and cell growth were enhanced by 36.23 and 44.29%, respectively. Also, the various nutritional parameters, which have significant effect on the production of alkaline protease, are delineated in the present study. Alkaline protease production was found to be influenced by media components, viz., types of C/N source and presence of metal ions. Finally an overall 6.2 fold increase in the production was achieved using casein as both carbon and nitrogen source.

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Section: Introductionmentioning

confidence: 98%

Effect of physical parameters, carbon and nitrogen sources on the production of alkaline protease from a newly isolatedBacillus pseudofirmus SVB1

2009

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“…The orbital agitation speed is important in the microbial synthesis of protease enzymes because this parameter affects the amount of dissolved oxygen. As dissolved oxygen is the rate-limiting factor because of its low solubility in the aqueous solution, it affects the cell grown and yield of products in the aerobic fermentation (Potumarthi et al, 2007). There is no general medium for protease production by different microbial strains.…”

Section: Resultsmentioning

confidence: 99%

COLLAGENASE PRODUCTION BY YEAST (13II) ISOLATED FROM BEE POLLEN (Melípona spp.)

Luiz¹,

Arruda²,

Moreira³

et al. 2015

Anais Do XX Congresso Brasileiro De Engenharia Química

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-Collagenases are enzymes with various important industrial and biotechnological applications. To be economically feasible, collagenase production should have high yields and use an inexpensive medium. The aim of this work was to study the influence of substrate concentration, pH and orbital agitation speed on collagenase production by yeast (13II) isolated from bee pollen (Melípona spp.) using a 2 3 full factorial design. The experiments were performed in 250mL Erlenmeyer flasks containing 50mL of culture medium inoculated with 10 6 cells/mL and incubated for 72h at 28 °C. Substrate concentration and initial medium pH exerted significant positive main effects, while the orbital agitation speed exerted a negative effect on collagenase production. The most favorable production conditions were found to be with 1.25% of gelatin, pH 7.5 and 100 rpm, which led to a collagenase activity of 66.86U. These results show that the yeast was found to be able to produce a large amount of colagenase.

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“…10 on plotting C L against [q O 2 Á C X þ ðdC L dt = Þ], the slope becomes equal to the reciprocal of k L a. The procedure was repeated several times during the production process (Potumarthi et al 2007). Microbial heat evolution data would be used indirectly to determine biomass concentration and OUR.…”

Section: Energy Dissipation Per Circulation Function and Aeration Nummentioning

confidence: 99%

Impact of aeration and agitation on metabolic heat and protease secretion of Aspergillus tamarii in a real-time biological reaction calorimeter

Dhandapani

Surianarayanan

Dhilipkumar

et al. 2012

Appl Microbiol Biotechnol

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The effects of aeration and agitation on metabolic heat, alkaline protease production and morphology for Aspergillus tamarii MTCC5152 are reported in this manuscript. Measurement of metabolic heat has been attempted by the continuous and dynamic heat balance method in a biological real-time reaction calorimeter. At lower agitation intensities, growth-related processes were dominating. As a result the protease activity and the product heat yields were lower than those for 350 and 450 rpm. Although biomass growth was necessary to obtain maximum protease yield, agitation seemed to play a vital role in the protease production process. Energy dissipation per circulation function of the process is also deduced from power input. At optimal conditions, 350 rpm and 1 vvm, the gassed power required was 0.133 W. Pellet morphology and protease production were studied under different aeration and agitation intensities of A. tamarii. Pellet structure was considerably influenced by DO, a higher DO level resulted in denser pellets (1,018.4 kg/m(3)) leading to higher protease activity. Coupling of hydrodynamics and bio-reaction highlighted the complex relationship between energy dissipation, substrate uptake rate and fungal physiology. This study emphasised the potential of biocalorimetry as a reliable monitoring and robust control tool for aerobic fermentation of A. tamarii, using agricultural by-products.

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Alkaline protease production by submerged fermentation in stirred tank reactor using Bacillus licheniformis NCIM-2042: Effect of aeration and agitation regimes

Cited by 109 publications

References 28 publications

Effect of physical parameters, carbon and nitrogen sources on the production of alkaline protease from a newly isolatedBacillus pseudofirmus SVB1

Effect of physical parameters, carbon and nitrogen sources on the production of alkaline protease from a newly isolatedBacillus pseudofirmus SVB1

COLLAGENASE PRODUCTION BY YEAST (13II) ISOLATED FROM BEE POLLEN (Melípona spp.)

Impact of aeration and agitation on metabolic heat and protease secretion of Aspergillus tamarii in a real-time biological reaction calorimeter

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