Alkaloid analysis by high-performance liquid chromatography-solid phase extraction-nuclear magnetic resonance: New strategies going beyond the standard

Mass spectrometry and NMR spectroscopy: modern high-end detectors for high resolution separation techniques – state of the art in natural product HPLC-MS, HPLC-NMR, and CE-MS hyphenations

“…87 Herein lies one of the major drawbacks of HPLC-SPE-NMR. 109 Any analyte which cannot be precipitated on the SPE stationary phase aer already eluting from the HPLC unit is lost for the subsequent transfer to the NMR spectrometer. In such cases, i.e.…”

Layer-by-layer functionalized porous Zinc sulfide nanospheres-based solid-phase extraction combined with liquid chromatography time-of-flight/mass and gas chromatography-mass spectrometry for the specific enrichment and identification of alkaloids from Crinum asiaticum var. sinicum

“…As indicated by Bieri et al [6], which could be due to problem with unsatisfactory capturing capacity for the charged alkaloids on the modified polymer sorbent (GP resin) and C8 or C18 end-capped material cartridge. They are generally regarded as the best overall sorbents for trapping and eluting a broad spectrum of natural products [12,13]. A study by Lee et al [13,14] found that only 56.2% of (þ)-boldine could be trapped on GP resin and 74.4% retention on C8 end-capped material.…”

“…They are generally regarded as the best overall sorbents for trapping and eluting a broad spectrum of natural products [12,13]. A study by Lee et al [13,14] found that only 56.2% of (þ)-boldine could be trapped on GP resin and 74.4% retention on C8 end-capped material. Alkaloids usually exist as positively charged compounds in crude plant extracts, so neutral GP resin and C8 matrix could not capture specific alkaloids well only based on "like dissolves like" principle [15,16].…”

Assessment of the Use of Natural Extracted Dyes and Pancreatin Enzyme for Dyeing of Four Natural Textiles: HPLC Analysis of Phytochemicals

“…High-performance liquid chromatography (HPLC), an analytical chemistry technique, was widely use to separate as well as to identify and quantify the components in a mixture of phenolics, flavonoids, alkaloids, polysaccharides and saponins [1][2][3][4]. Phenolic and flavonoids compounds extracted from natural materials play a potential role in dyeing of natural fibers under the work enzymes [5,6].…”