All-fiber biological detection microfluidic chip based on space division and wavelength division multiplexing technologies

Wei, Yong; Ren, Zhuo; Liu, Chunlan; Jiang, Tianci; Wang, Rui; Shi, Chen; Liu, Chunbiao

doi:10.1039/d2lc00681b

Cited by 8 publications

(1 citation statement)

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“…Optical biosensors have long attracted great attention for the measurement of trace targets due to their high sensitivity, rapidity, portability, and cost-effectiveness [20,21]. In this study, a portable evanescent wave fluorescence biosensor was built for rapid and sensitive on-site detection of FIP based on a highly specific monoclonal antibody (Figure 1).…”

Section: Introductionmentioning

confidence: 99%

Rapid and Sensitive On-Site Detection of Fipronil in Foods Using Evanescent Wave Fluorescent Immunosensor

Li,

Xu,

Liu

et al. 2023

Chemosensors

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Fipronil (FIP), a broad-spectrum phenylpyrazole insecticide, is highly toxic and threatens human health and ecological balance. Developing convenient, rapid, portable analytical technology for on-site and high-frequency testing of FIP is essential to reduce its damage. Herein, a monoclonal antibody (Clone F-3F6) against FIP, with high affinity and specificity, was produced using a novel immunogen, FIP-BSA, which was simply and directly synthesized by conjugating FIP with bovine serum albumin (BSA). Among the previously reported antibodies, F-3F6 acts more specifically against FIP. The FIP metabolites fipronil desulfinyl, fipronil sulfide, and fipronil sulfone showed lower cross-reactivity, and other pesticides were not recognized. To achieve high-frequency and on-site measurements of FIP, an evanescent wave fluorescence biosensor was built by integrating evanescent wave fluorescence technology, a functionalized fiber bioprobe, and a fluorescence-labeled F-3F6 antibody. The detection limit of FIP was 0.032 μg/L. The detection results of real milk and water samples showed that all the coefficients of variation were less than 10%, and the recovery ranged from 90 to 120%. The high reusability and stability of functionalized fiber bioprobe enables the accurate, cost-effective, high-frequency, and facile quantitative detection of FIP. This highly specific and reliable evanescent wave fluorescence biosensor will be well suited to the sensitive and high-frequency on-site analysis of only FIP in food.

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Section: Introductionmentioning

confidence: 99%