Allelic variability in the third intron of the fibroin light chain gene in Bombyx mori (Lepidoptera: Bombycidae)

Barbosa, Joice F; Bravo, Juliana Pereira; Zanatta, Daniela B; Silva, J.L.C.; Fernandez, M. A.

doi:10.4238/vol8-1gmr543

Cited by 3 publications

(5 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A pool of first-instar larvae from both strains (M11A and C24A) were macerated with liquid nitrogen, and DNA was extracted as previously described (Barbosa et al, 2008(Barbosa et al, , 2009. DNA fragments were amplified using the primers described in the DNA sample section.…”

Section: Conformation-sensitive Gel Electrophoresis -Csgementioning

confidence: 99%

“…DNA fragments were amplified using the primers described in the DNA sample section. As a control, the third intron of the B. mori light fibroin chain gene was amplified from C122B and C25B strains, as described elsewhere (Barbosa et al, 2009). The C122B strain is negative for the heteroduplex genotype (Barbosa JB, personal communication), while the C25B strain presents a positive result for the CSGE technique (Barbosa et al, 2009).…”

Section: Conformation-sensitive Gel Electrophoresis -Csgementioning

confidence: 99%

“…As a control, the third intron of the B. mori light fibroin chain gene was amplified from C122B and C25B strains, as described elsewhere (Barbosa et al, 2009). The C122B strain is negative for the heteroduplex genotype (Barbosa JB, personal communication), while the C25B strain presents a positive result for the CSGE technique (Barbosa et al, 2009). Screening for polymorphisms using heteroduplex analyses from all strains was performed by denaturation of polymerase chain reaction (PCR) products at 95°C for 10 min followed by hybridization for 1 h at 60°C.…”

Section: Conformation-sensitive Gel Electrophoresis -Csgementioning

confidence: 99%

“…Genomic DNA from third-instar larvae of each strain was purified, and the first intron of the A3 actin gene was amplified and analyzed by CSGE. C-and C+ indicate negative and positive controls to amplified segments of the third intron of the fibroin light chain gene from strains C122B and C25B, as described by Barbosa et al (2009). A 1% agarose gel (AGA) was useful as a control because allele differences are not identified in this system.…”

Section: J Tavares Et Almentioning

confidence: 99%

See 3 more Smart Citations

Differential structure of the intronic promoter of the Bombyx mori A3 actin gene correlated with silkworm sensitivity/resistance to nucleopolyhedrovirus

Tavares

Bravo²,

Gimenes³

et al. 2011

Genet. Mol. Res.

Self Cite

View full text Add to dashboard Cite

ABSTRACT. Previous reports demonstrated that actin is necessary for nucleocapsid transport and viral gene expression during nucleopolyhedrovirus infection of Bombyx mori. The first intron of B. mori A3 actin contains a cryptic promoter that drives expression of a rare isoform. We detected differences in the size and nucleotide composition of the first intron of the A3 actin gene from B. mori strain C24A, which is more resistant to nucleopolyhedrovirus than the M11A strain (22 and 95% lethality, respectively). We sought to determine if resistance to BmMNPV infection and the A3 actin promoter structure are correlated. Intrinsically bent DNA sites in these sequences, which determine curved structures, were analyzed by electrophoretic mobility assays and the helical parameters ENDS ratio, roll and twist. We found both fragments to have non-centralized bent DNA sites with distinct ENDS ratio values, nucleotide positions and two-dimensional structures. Additionally, a conformational-sensitive gel electrophoresis assay identified an allelic variation found in strain M11A that is absent in strain C24A. These data suggest that A3 actin intronic sequence variations impair virus propagation and are markers of BmMNPV-resistant populations.

show abstract

Section: Conformation-sensitive Gel Electrophoresis -Csgementioning

confidence: 99%

Section: Conformation-sensitive Gel Electrophoresis -Csgementioning

confidence: 99%

Section: Conformation-sensitive Gel Electrophoresis -Csgementioning

confidence: 99%

Section: J Tavares Et Almentioning

confidence: 99%

See 2 more Smart Citations

Differential structure of the intronic promoter of the Bombyx mori A3 actin gene correlated with silkworm sensitivity/resistance to nucleopolyhedrovirus

Tavares

Bravo²,

Gimenes³

et al. 2011

Genet. Mol. Res.

Self Cite

View full text Add to dashboard Cite

show abstract

“…FIBH has one intron and two large exons, while Fib-l gene has seven exons separated by large introns. The latter gene mostly contains non-coding DNA as the first intron represents 60% of the gene, and the rest of the introns add up to 31% (Barbosa et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Novel insights into the economic relevant phenotypes ofBombyx moristrains from Romanian breeding centres

Baci,

Ionascu,

Ratiu

et al. 2024

Preprint

View full text Add to dashboard Cite

The silkworm,Bombyx mori, has an inestimable economic and pharmaceutical value, but it is also a very effective model organism for biological and biotechnological sciences. Herein, we characterize several phenotypic parameters particular for Băneasa 1 (B1) and Galben de Băneasa (GB) Romanian silkworm breeds, as well as of Auriu Chinez (ACH) and JH3 breeds originating from China and, respectively, Japan. Our analysis revealed concrete differences and similarities between the phenotypic properties of the four breeds, allowing their grouping consecutive to PCA and KODAMA analyses. Our study is the first to characterize the four breeds regarding their biological competencies and offers a glimpse at various ventures aiming at the employment and amelioration of these silkworm breeds.

show abstract

Allelic Variability in the Intronic Region of the Fibroin Heavy-Chain Gene in Silkworm Bombyx mori L. Strains of Brazilian Germplasm Bank

et al. 2014

View full text Add to dashboard Cite

The domesticated silkworm Bombyx mori L. is currently found only in germplasm banks. Therefore, characterization and conservation of this genetic resource is crucial. Based on previous studies that revealed nucleotide differences in silkworm strains, the intron of the fibroin heavy chain gene (H-fib) can be used for molecular silkworm characterization. The H-fib gene has two exons and a unique intron, and encodes the principal component of the silk fiber, the fibroin heavy chain. Therefore, this study aimed to identify the genetic variability of the unique intron of H-fib gene of 20 silkworm strains maintained at the Universidade Estadual de Maringá Brazilian Germplasm Bank (UBGB) by conformation-sensitive gel electrophoresis (CSGE) and nucleotide sequencing. Genomic DNA extracted from silkworm moths was PCR amplified. CSGE revealed that most of the analyzed silkworm strains had only homoduplex molecules. However, DNA from the Japanese strains B106, B82, and M12-2 had two extra DNA fragments produced by heteroduplex molecules, revealing variation between alleles. Sequencing of the H-fib intron was used to confirm the variation previously detected by CSGE and detected a significant polymorphism characterized by a 17-base pair (bp) deletion, a 2-bp insertion, and eight nucleotide substitutions. Although genetic and allelic variability was detected in some silkworm strains, the intron of the H-fib gene revealed not to be the best molecular marker for the characterization of B. mori strains from UBGB.

show abstract

Allelic variability in the third intron of the fibroin light chain gene in Bombyx mori (Lepidoptera: Bombycidae)

Cited by 3 publications

References 22 publications

Differential structure of the intronic promoter of the Bombyx mori A3 actin gene correlated with silkworm sensitivity/resistance to nucleopolyhedrovirus

Differential structure of the intronic promoter of the Bombyx mori A3 actin gene correlated with silkworm sensitivity/resistance to nucleopolyhedrovirus

Novel insights into the economic relevant phenotypes ofBombyx moristrains from Romanian breeding centres

Allelic Variability in the Intronic Region of the Fibroin Heavy-Chain Gene in Silkworm Bombyx mori L. Strains of Brazilian Germplasm Bank

Contact Info

Product

Resources

About