Allelopathic activity of Dendrophthoe pentandra as a potential bioherbicide to inhibit seed germination and seedling growth of Eleusine indica

Abstract

Abstract. Alharits L, Hemelda NM, Yasman, Handayani W. 2020. Allelopathic activity of Dendrophthoe pentandra as a potential bioherbicide to inhibit seed germination and seedling growth of Eleusine indica. Nusantara Bioscience 12: 33-39. Dendrophthoe pentandra (L.) Miq. is a common parasitic plant in Southeast Asia. As a parasitic plant, it exhibits phytochemicals important for infecting its host, which may be potential as bioherbicide. This study was aimed to evaluate allelopathic activity of D. pentandra (stem and leaf extracts) to control Eleusine indica (L.) Gaertn. seed germination and growth. Methanolic extracts of D. pentandra stem and leaf (0, 1, 2, 3, 4 and 5 mg/mL in water) were treated on E. indica seeds in a petri dish for 10 days. After 10 days, germination of treated E. indica seeds was significantly reduced and reached >80% inhibition at 5 mg/mL. Moreover, E. indica roots were more sensitive compared to E. indica shoots, indicated by root inhibition >50% at 1 mg/mL and reached >90% inhibition at 5 mg/mL. Leaf extract presented stronger inhibition than stem extract, but not significant. It may be related to the higher accumulation of phenolic and flavonoid compounds in leaf extract than in stem extract. This is the first report of allelopathic activity in D. pentandra to inhibit E. indica germination and growth. Therefore, further study in a greenhouse, as well as metabolism effects of D. pentandra on the target weeds are important to provide comprehensive evaluation of D. pentandra potential as bioherbicide.

A Review of Dendrophthoe pentandra (Mistletoe): Phytomorphology, Extraction Techniques, Phytochemicals, and Biological Activities

“…Almost all the parts of the D. pentandra have been used traditionally for traditional medicinal and alternative medicinal purposes. For example, the extract from D. pentandra leaves has been used for diabetic and aging-related diseases due to its antidiabetic [1,19] and antioxidant properties [1,[4][5][6][7][8][9][10]. Moreover, D. pentandra flowers, when extracted, have also been studied due to their antioxidant properties [5].…”

“…For example, the extract from D. pentandra leaves has been used for diabetic and aging-related diseases due to its antidiabetic [1,19] and antioxidant properties [1,[4][5][6][7][8][9][10]. Moreover, D. pentandra flowers, when extracted, have also been studied due to their antioxidant properties [5]. Interestingly, Pramestya et al [29] found that D. pentandra flowers tend to accumulate more secondary metabolites than other parts.…”

“…Although D. pentandra is a semi-parasitic plant, its bioactivities may also depend on its host plant [3]. D. pentandra has been reported to have a high potential for medicinal values such as antioxidants [1,[4][5][6][7][8][9][10], antibacterial [6,7], anticancer, antiproliferative [8,[11][12][13][14][15][16][17][18], antidiabetic, antihyperglycaemic [1,19], anti-inflammatory [20], cytotoxicity [1,14,16], hepatoprotective [19], immunomodulatory [21], and anti-aging [22] activities. The phytochemical analysis of D. pentandra's leaves and flowers also revealed the presence of alkaloids, phenolics, flavonoids, saponins, and terpenoids [5,10,16,23].…”

Dendrophthoe falcata (L.f.) Ettingsh. and Dendrophthoe pentandra (L.) Miq.: A review of traditional medical uses, phytochemistry, pharmacology, toxicity, and applications

“…In folklore, DF is called “Banda” ( Ghirtlahre et al, 2015 ) and “Vanda” ( Khatoon et al, 2011 ), while DP is called “Benalu” ( Elsyana et al, 2016 ). Additionally, DF and DP are also commonly known as Indian mistletoe and Indonesian mistletoe, respectively in Englishbecause their distribution is concentrated in India and Indonesia, respectively ( Sinoriya et al, 2011 ; Lim et al, 2017 ; Alharits et al, 2020 ; Singh et al, 2020 ; Bhagat and Kondawara, 2021 ; Yapa et al, 2021 ). Moreover, they are rarely present in other countries like Thailand ( Kwanda et al, 2013 ), China ( Guo and Ren, 2019 ), and Bangladesh ( Hasan et al, 2018 ).…”

“…concentrated in India and Indonesia, respectively (Sinoriya et al, 2011;Lim et al, 2017;Alharits et al, 2020;Singh et al, 2020;Bhagat and Kondawara, 2021;Yapa et al, 2021). Moreover, they are rarely present in other countries like Thailand (Kwanda et al, 2013), China (Guo and Ren, 2019), and Bangladesh (Hasan et al, 2018).…”