Allergen Component Specific IgE Measurement With the Immulite™ 2000 System: Diagnostic Accuracy and Intermethod Comparison

Abstract

A high diagnostic accuracy of the sIgE to allergen components measurement with Immulite™ 2000 and a high agreement with ImmunoCAP™ platforms were shown in this study.

Allergen‐Specific IgE Measurement: Intermethod Comparison of Two Assay Systems in Diagnosing Clinical Allergy

“…We have detected fair to excellent correlation and agreement between these two assays, despite their methodological differences for most of the tested allergens, but also found inter-and intra-assay discordance for some allergens, which is consistent with previous studies. As this discordance was different for different allergens, it does not seem to occur due to the systemic differences between these two assays but was more likely to be connected with diversity and quality of allergenic extracts used (12,(23)(24)(25).…”

Correlations Between Allergen-Specific IgE Serum Levels in Patients With Ocular Allergy

“…The overall agreement between Immulite 2000 and ImmunoCAP platforms was 98.6%. 21 Most studies regarding IgE detection methods demonstrate excellent results for parameters such as reproducibility, accuracy, and linearity. The similarities observed between some techniques, especially ImmunoCAP and Immulite, lead us to conclude that both methods are equivalent.…”

Precision medicine allergy immunoassay methods for assessing immunoglobulin E sensitization to aeroallergen molecules

“…This automated quantitative chemiluminescent method can also be used in molecular allergy diagnostics. It has the following steps[ 42 , 53 , 61 ]: (1) Specific IgE binding in liquid-phase step: Native purified allergen component covalently bound to soluble biotinylated polylysine polymer in a fluid phase binds to streptavidin-coated polystyrene bead (as solid-phase) in the reaction tube (through a streptavidin-biotin interaction) and reacts with specific IgE from the patient’s serum sample (during the incubation of streptavidin-coated bead, biotinylated liquid allergen, and patient sample); (2) Conjugate/labeled anti-IgE detection antibody step: After spin washing, alkaline phosphatase enzyme-labeled anti-human IgE monoclonal murine antibody is added to form the so-called antigen-antibody immune complex; (3) Chemiluminescent signal step: After the bead is washed again (efficient washing with spinning at high speed of the tube on its vertical axis), adamantyl 1,2-dioxetane aryl phosphate is added as chemiluminescent substrate. In the luminogenic reaction, the action of bound alkaline phosphatase on this stable substrate creates an unstable adamantly dioxetane anion, with its rapidly and spontaneously breakdown emitting a photon of light; and (4) Chemiluminescence measurement: Performed by a photon-counting photomultiplier tube/luminometer, and there is a correlation between the chemiluminescent signal and the allergen-bound IgE established from a standard curve of concentration points.…”

“…The detection limits are 0.10-100 kU A /L, and the sample volume is 50 µL. A high diagnostic accuracy of the specific IgE to allergen components measurement with this Immulite ® 2000 system and a high agreement with ImmunoCAP ® platforms were revealed[ 61 ]. An important difference between these methods is the source and quality of the allergenic extracts used[ 53 ].…”