Allergen Screening Bioassays: Recent Developments in Lab-On-A-Chip and Lab-On-A-Disc Systems

Ho, Ho‐Pui; Lau, Pui-man; Kwok, Ho-Chin; Wu, S.Y.; Gao, Minghui; Cheung, Anthony Ka-lun; Wang, Guanghui; Kwan, Yiu Wa; Wong, Chun‐Kwok; Kong, Siu‐Kai

doi:10.4155/bio.14.153

Cited by 8 publications

(3 citation statements)

References 67 publications

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“…Some affinity separation techniques reviewed above are ready to be integrated with microfluidic system, such as magnetic beads. Though there are not yet reports on such system designed for active compounds screening, it is possible in theory due to the common procedures of incubation, wash, and elution that already existed [71]. Lab-on-a-chip or lab-on-a-CD is another feasible strategy for high-throughput screening, which would contribute significantly to chemical and pharmacological research of TCM.…”

Section: Future Perspectives Of Ligand Fishing For Tcmmentioning

confidence: 99%

Ligand Fishing: A Remarkable Strategy for Discovering Bioactive Compounds from Complex Mixture of Natural Products

et al. 2016

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Identification of active compounds from natural products is a critical and challenging task in drug discovery pipelines. Besides commonly used bio-guided screening approaches, affinity selection strategy coupled with liquid chromatography or mass spectrometry, known as ligand fishing, has been gaining increasing interest from researchers. In this review, we summarized this emerging strategy and categorized those methods as off-line or on-line mode according to their features. The separation principles of ligand fishing were introduced based on distinct analytical techniques, including biochromatography, capillary electrophoresis, ultrafiltration, equilibrium dialysis, microdialysis, and magnetic beads. The applications of ligand fishing approaches in the discovery of lead compounds were reviewed. Most of ligand fishing methods display specificity, high efficiency, and require less sample pretreatment, which makes them especially suitable for screening active compounds from complex mixtures of natural products. We also summarized the applications of ligand fishing in the modernization of Traditional Chinese Medicine (TCM), and propose some perspectives of this remarkable technique.

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Section: Future Perspectives Of Ligand Fishing For Tcmmentioning

confidence: 99%

Ligand Fishing: A Remarkable Strategy for Discovering Bioactive Compounds from Complex Mixture of Natural Products

et al. 2016

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“…Flow control is achieved by varying the spinning speed, spinning direction, and operations such as valve opening, siphoning, volume metering, mixing, and flow switching. In our previous studies, a LOAD platform was built for allergy testing [ 16 , 17 ]. In our second generation LOAD, the AO-loaded cells that were sensitized with a patient’s serum or medium alone were challenged with suspected allergens or other triggers in chamber at body temperature (37 °C).…”

Section: Introductionmentioning

confidence: 99%

Allergy Testing and Drug Screening on an ITO-Coated Lab-on-a-Disc

Kwok

Lau

et al. 2016

Micromachines

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A lab-on-a-disc (LOAD) is a centrifugal microfluidic set-up based on centrifugal force without using micro-pumps to drive reagents and cells to various chambers through channels and valves for reactions. A LOAD coated with conductive transparent indium tin oxide (ITO) for thermal control was developed to screen allergy-blocking agents. When the acridine orange (AO)-loaded KU-812 human basophilic cells were activated in the LOAD by stimuli, AO trapped in the cytoplasmic granules was released externally as an allergic mediator mimetic to report degranulation. This response was monitored by fluorescence when the released AO in supernatant had been transferred, with a higher spinning speed, from the reaction chamber to detection chamber in the LOAD where AO reacted with exogenous DNA. We report here the principles of the system and an improved LOAD set-up with the ITO-coated glass resistive microheater to run assays at 37 °C. By using this platform, we demonstrate here for the first time that triptolide, an active ingredient from the Chinese medicine herb Tripterygium wilfordii Hook f., was able to suppress the fMLP-mediated degranulation in basophils. This serves as an example how LOADs can be used to screen agents to alleviate symptoms of allergy.

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“…One obvious point of development is towards low-cost, portable and disposable point of care diagnostic (POC) devices. [1][2][3][4] In virtually all POC implementations, there is a need for a multifunction matrix material onto which operations are affected. Many polymeric materials such as polyethylene, nitrocellulose (NC), Dacron, polyvinyl chloride (PVC), nylon, polyacrylonitrile (PAN) etc.…”

Section: Introductionmentioning

confidence: 99%

Parallel, open-channel lateral flow (immuno) assay substrate based on capillary-channeled polymer films

Zhang

Jiang

Willett

et al. 2016

Analyst

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Presented here is a novel implementation of polypropylene capillary-channeled polymer (C-CP) films, functionalized for bioaffinity separations and implemented as a platform for lateral flow (immuno) assays. The parallel ∼80 μm × 80 μm channels pass test solutions down the 30 mm film length via spontaneous wicking action, setting up the possibility for immobilizing different capture agents in the respective channels. The base-film modification process is divided into two steps: ultraviolet light treatment to improve hydrophillicity of the polypropylene substrate and the physical adsorption of a functionalized lipid tethered ligand (LTL) as a selective capture agent. The entire modification procedure is performed under ambient conditions in an aqueous solution without extreme pH conditions. In this demonstration, physical adsorption of a biotinylated-LTL onto the UV-treated PP surface selectively captures Texas Red-labeled streptavidin (SAv-TR) in the presence of enhanced green fluorescence protein (EGFP), which passes without retention in less than 5 s. In addition to the fluorescence imaging of the protein solutes, matrix assisted laser desorption/ionization-mass spectrometry (MALDI-MS) was used to confirm the formation of the LTL-SAv conjugates on the channel surface as well as to demonstrate an alternative means of probing the capture step. The present effort sets the groundwork for further development of C-CP films as a parallel, multi-analyte LFA platform; a format that to-date has not been described.

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Allergen Screening Bioassays: Recent Developments in Lab-On-A-Chip and Lab-On-A-Disc Systems

Cited by 8 publications

References 67 publications

Ligand Fishing: A Remarkable Strategy for Discovering Bioactive Compounds from Complex Mixture of Natural Products

Ligand Fishing: A Remarkable Strategy for Discovering Bioactive Compounds from Complex Mixture of Natural Products

Allergy Testing and Drug Screening on an ITO-Coated Lab-on-a-Disc

Parallel, open-channel lateral flow (immuno) assay substrate based on capillary-channeled polymer films

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