Allergens and Molecular Diagnostics of Shellfish Allergy

Lopata, Andreas L.; Kleine-Tebbe, J.; Kamath, Sandip D.

doi:10.1007/978-3-319-42499-6_21

Cited by 23 publications

(32 citation statements)

References 51 publications

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“…However, there were insufficient data on the consumption of these commodities between the two areas to postulate FA risk. The high incidence of shellfish allergy in urban children might be related to higher exposure to indoor allergens as discussed in the current literature . For instance, indoor mites were documented to cross‐react with the major shellfish allergen tropomyosin, and storage mites were identified in indoor environments in the north of Vietnam .…”

Section: Discussionmentioning

confidence: 96%

A cross‐sectional, population‐based study on the prevalence of food allergies among children in two different socio‐economic regions of Vietnam

Nguyen

et al. 2019

Pediatric Allergy Immunology

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Background There is a paucity of data on the prevalence of food allergy (FA) in Vietnam. A cross‐sectional, population‐based study was conducted to evaluate the current prevalence of FA among 2‐ to 6‐year‐old children in two different regions in Vietnam. Method A structured, anonymous questionnaire, modified from published FA epidemiologic studies and based on EAACI guidelines, was distributed to parents/guardians of participating children in Hue City (urban area) and Tien Giang Province (rural area). Data collected from the survey were statistically analyzed to generate the prevalence of self‐reported and doctor‐diagnosed FA and overarching pattern of food allergens. Results A total of 8620 responses were collected (response rate 81.5%). Children in Tien Giang reported more than twice the food‐induced adverse reactions seen in children in Hue (47.8% vs. 20.5%). In contrast, children in Hue showed higher self‐reported FA (9.8%) and doctor‐diagnosed FA rates (8.4%) than children in Tien Giang (7.9% and 5.0%, respectively). Crustacean was the predominant allergy‐inducing food in both areas (330 of 580 cases, 56.9%), followed by fish, mollusk, beef, milk, and egg. However, substantial variations of FA patterns were seen between the study sites. Geographic location and co‐morbidities of other allergic diseases were key risk factors for FA (P < 0.001). Conclusions The prevalence of FA in Vietnamese children seems to be higher than previously reported from other Asian countries. Crustacean is the predominant allergy‐inducing food among participating preschool children in Vietnam. The variation of reported food allergen sources across different socio‐economic locations could imply different eating habits or the participation of indoor and outdoor allergen exposure.

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Section: Discussionmentioning

confidence: 96%

A cross‐sectional, population‐based study on the prevalence of food allergies among children in two different socio‐economic regions of Vietnam

Nguyen

et al. 2019

Pediatric Allergy Immunology

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“…Troponin-like protein (UniProt ID: A0A0M3JU57), a potential allergen detected in this study, and its homolog (Ani s troponin C) also contain EF-hand domains. EF-hand allergen family is the second-largest group of allergens [ 77 ] that are detected in different organisms like parvalbumins in a specific species of fish and fungus ( Trichophyton violaceum ) [ 78 ] or polcalcins in the pollen of trees, grasses, and weeds [ 79 ]. It has been found that antigenic sites of parvalbumins in Trichophyton violaceum are located on both sides of the Ca 2+ -binding site of the first EF-hand domain and parvalbumin proteins possessing conserved amino acid motifs (cysteine, lysine, and arginine) [ 78 ].…”

Section: Discussionmentioning

confidence: 99%

Proteomic and Bioinformatic Investigations of Heat-Treated Anisakis simplex Third-Stage Larvae

et al. 2020

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Anisakis simplex third-stage larvae are the main source of hidden allergens in marine fish products. Some Anisakis allergens are thermostable and, even highly processed, could cause hypersensitivity reactions. However, Anisakis proteome has not been studied under autoclaving conditions of 121 °C for 60 min, which is an important process in the food industry. The aim of the study was the identification and characterization of allergens, potential allergens, and other proteins of heat-treated A. simplex larvae. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to identify 470 proteins, including allergens—Ani s 1, Ani s 2, Ani s 3, Ani s 4, Ani s 5—and 13 potential allergens that were mainly homologs of Anisakis spp., Ascaris spp., and Acari allergens. Ani s 2, Ani s 3, Ani s 5, and three possible allergens were found among the top 25 most abundant proteins. The computational analysis allowed us to detect allergen epitopes, assign protein families, and domains as well as to annotate the localization of proteins. The predicted 3D models of proteins revealed similarities between potential allergens and homologous allergens. Despite the partial degradation of heated A. simplex antigens, their immunoreactivity with anti-A. simplex IgG antibodies was confirmed using a Western blot. In conclusion, identified epitopes of allergenic peptides highlighted that the occurrence of Anisakis proteins in thermally processed fish products could be a potential allergic hazard. Further studies are necessary to confirm the IgE immunoreactivity and thermostability of identified proteins.

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“…222 Recently, several other molecules associated with IgE-mediated reactivity to crustaceans have been isolated in allergic patients. 223 Arginine kinase, sarcoplasmic calcium-binding protein, myosin light chain, myosin heavy chain, troponin C, glyceraldehyde-3-phosphate dehydrogenase, triosin phosphate isomerase, enolase, hemocyanin, Ca þþ ATPase of smooth endoplasmic reticulum, and pyruvate kinase are the most relevant. 224…”

Section: Shellfishmentioning

confidence: 99%

A WAO — ARIA — GA2LEN consensus document on molecular-based allergy diagnosis (PAMD@): Update 2020

Ansotegui¹,

Melioli²,

Canonica³

et al. 2020

World Allergy Organization Journal

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Precision allergy molecular diagnostic applications (PAMD@) is increasingly entering routine care. Currently, more than 130 allergenic molecules from more than 50 allergy sources are commercially available for in vitro specific immunoglobulin E (sIgE) testing. Since the last publication of this consensus document, a great deal of new information has become available regarding this topic, with over 100 publications in the last year alone. It thus seems quite reasonable to publish an update. It is imperative that clinicians and immunologists specifically trained in allergology keep abreast of the new and rapidly evolving evidence available for PAMD@. PAMD@ may initially appear complex to interpret; however, with increasing experience, the information gained provides relevant information for the allergist. This is especially true for food allergy, Hymenoptera allergy, and for the selection of allergen immunotherapy. Nevertheless, all sIgE tests, including PAMD@, should be evaluated within the framework of a patient's clinical history, because allergen sensitization does not necessarily imply clinical relevant allergies.

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Allergens and Molecular Diagnostics of Shellfish Allergy

Cited by 23 publications

References 51 publications

A cross‐sectional, population‐based study on the prevalence of food allergies among children in two different socio‐economic regions of Vietnam

A cross‐sectional, population‐based study on the prevalence of food allergies among children in two different socio‐economic regions of Vietnam

Proteomic and Bioinformatic Investigations of Heat-Treated Anisakis simplex Third-Stage Larvae

A WAO — ARIA — GA2LEN consensus document on molecular-based allergy diagnosis (PAMD@): Update 2020

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