Allergic sensitization modifies the pulmonary expression of 5-hydroxytryptamine receptors in guinea pigs

Córdoba-Rodríguez, Guadalupe; Vargas, Mario H.; Ruiz, Vı́ctor; Carbajal, Verónica; Campos-Bedolla, Patricia; Mercadillo-Herrera, Paulina; Arreola-Ramírez, José Luis; Segura-Medina, Patricia

doi:10.1016/j.resp.2015.11.018

Cited by 10 publications

(7 citation statements)

References 43 publications

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“…Moreover, this study is the first of its kind confirming the expression of sympatho-inhibitory 5-HT receptors in a peripheral sympathetic ganglion in both control (normoglycaemic) and type 1 diabetic rats. In agreement with the findings that demonstrate the peripheral presence of 5-HT 5A receptors, some authors have also analyzed their expression in peripheral tissues and structures with an important role in cardiovascular regulation 48,49 . However, the vast majority of studies have linked 5-HT 5A receptors with serotonergic effects within the central nervous system 50 .…”

Section: Discussionsupporting

confidence: 67%

Role of peripheral 5-HT5A receptors in 5-HT-induced cardiac sympatho-inhibition in type 1 diabetic rats

García-Pedraza

Hernández-Abreu

Morán

et al. 2020

Sci Rep

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5-HT inhibits cardiac sympathetic neurotransmission in normoglycaemic rats, via 5-HT1B, 5-HT1D and 5-HT5A receptor activation. Since type 1 diabetes impairs the cardiac sympathetic innervation leading to cardiopathies, this study aimed to investigate whether the serotonergic influence on cardiac noradrenergic control is altered in type 1 diabetic rats. Diabetes was induced in male Wistar rats by streptozotocin (50 mg/kg, i.p.). Four weeks later, the rats were anaesthetized, pithed and prepared for producing tachycardic responses by electrical preganglionic stimulation (C7-T1) of the cardioaccelerator sympathetic outflow or i.v. noradrenaline bolus injections. Immunohistochemistry was performed to study 5-HT1B, 5-HT1D and 5-HT5A receptor expression in the stellate ganglion from normoglycaemic and diabetic rats. In the diabetic group, i) i.v. continuous infusions of 5-HT induced a cardiac sympatho-inhibition that was mimicked by the 5-HT1/5A agonist 5-carboxamidotryptamine (without modifying noradrenaline-induced tachycardia), but not by the agonists indorenate (5-HT1A), CP 93,129 (5-HT1B), PNU 142633 (5-HT1D), or LY344864 (5-HT1F); ii) SB 699551 (5-HT5A antagonist; i.v.) completely reversed 5-CT-induced cardiac sympatho-inhibition; and iii) 5-HT5A receptors were more expressed in the stellate ganglion compared to normoglycaemic rats. These results show the prominent role of the peripheral 5-HT5A receptors prejunctionally inhibiting the cardiac sympathetic drive in type 1 diabetic rats.

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Section: Discussionsupporting

confidence: 67%

Role of peripheral 5-HT5A receptors in 5-HT-induced cardiac sympatho-inhibition in type 1 diabetic rats

García-Pedraza

Hernández-Abreu

Morán

et al. 2020

Sci Rep

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“…The situation in mice is also complicated by the fact that mouse FcγRIV acts as IgE-receptor similar to human FcεRI and contributes in IgE-induced lung inflammation [3]. More rarely, other allergy models than mice were used, for instance rats and guinea pigs [4,5], or piglets [6], especially for answering pharmacologic questions. In the investigated time period of this review (18 months back), no research articles involving monkey or nonhuman primates were published.…”

Section: Introductionmentioning

confidence: 99%

Outstanding animal studies in allergy I. From asthma to food allergy and anaphylaxis

Jensen‐Jarolim

Pali‐Schöll

Roth‐Walter

2017

Current Opinion in Allergy &Amp; Clinical Immunology

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Purpose of reviewAnimal models published within the past 18 months on asthma, food allergy and anaphylaxis, all conditions of rising public health concern, were reviewed.Recent findingsWhile domestic animals spontaneously develop asthma, food allergy and anaphylaxis, in animal models, divergent sensitization and challenge routes, dosages, intervals and antigens are used to induce asthmatic, food allergic or anaphylactic phenotypes. This must be considered in the interpretation of results. Instead of model antigens, gradually relevant allergens such as house dust mite in asthma, and food allergens like peanut, apple and peach in food allergy research were used. Novel engineered mouse models such as a mouse with a T-cell receptor for house dust mite allergen Der p 1, or with transgenic human hFcγR genes, facilitated the investigation of single molecules of interest. Whole-body plethysmography has become a state-of-the-art in-vivo readout in asthma research. In food allergy and anaphylaxis research, novel techniques were developed allowing real-time monitoring of in-vivo effects following allergen challenge. Networks to share tissues were established as an effort to reduce animal experiments in allergy which cannot be replaced by in-vitro measures.SummaryNatural and artificial animal models were used to explore the pathophysiology of asthma, food allergy and anaphylaxis and to improve prophylactic and therapeutic measures. Especially the novel mouse models mimicking molecular aspects of the complex immune network in asthma, food allergy and anaphylaxis will facilitate proof-of-concept studies under controlled conditions.

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“…In contrast, mRNA expression of the 5-HT 2A receptor in CD4+ T-cells is reduced in patients with asthma as compared with healthy subjects (Chen et al 2016). In animal models, the protein expression of 5-HT 2A and 5-HT 4 receptors in guinea pig lung was found to be increased after systemic sensitization with ovalbumin (OVA) (Cordoba-Rodriguez et al 2016).…”

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confidence: 99%

Allergic Sensitization Increases Contractile Responses to 5-HT in Guinea Pig Aorta

et al. 2020

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Epidemiological and clinical studies suggest that asthma is associated with adverse cardiovascular outcomes, but its mechanism is uncertain. 5-Hydroxytryptamine (5-HT) is a mediator involved in asthma and in cardiovascular functioning. Thus, in the present study, we explored whether allergic sensitization in guinea pigs modifies 5-HT-induced contractile responses and 5-HT2A receptor expression in thoracic aorta rings. We found that sensitization produced a significant increase of 100 µM 5-HT-induced contractions of aorta rings (~27 % greater contraction than in non-sensitized animals, p<0.05). Preincubation with 10 nM ketanserin (a 5-HT2A receptor antagonist) reduced by ~30 % (p=0.003) and ~36 % (p=0.005) the area under the curve of 5-HT-induced contractions in aortas from non-sensitized and sensitized animals, respectively. There were no differences between sensitized and non-sensitized animals with respect to mRNA (qPCR) and protein (Western blot) expression of 5-HT2A receptor in thoracic aortas. We concluded that in this guinea pig model of asthma, allergic sensitization is not confined to airways, but also affects arterial contractile responses to 5-HT; changes in the expression of the 5-HT2A receptor appear not to be involved in this phenomenon.

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Allergic sensitization modifies the pulmonary expression of 5-hydroxytryptamine receptors in guinea pigs

Cited by 10 publications

References 43 publications

Role of peripheral 5-HT5A receptors in 5-HT-induced cardiac sympatho-inhibition in type 1 diabetic rats

Role of peripheral 5-HT5A receptors in 5-HT-induced cardiac sympatho-inhibition in type 1 diabetic rats

Outstanding animal studies in allergy I. From asthma to food allergy and anaphylaxis

Allergic Sensitization Increases Contractile Responses to 5-HT in Guinea Pig Aorta

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