Allium cepa exerts neuroprotective effect on retinal ganglion cells of pterygopalatine artery (PPA) ligated mice

Abstract

Background Repeated failure to rescue the damaged retinal ganglion cells (RGCs) by various drugs has warranted the need to screen common herbal compounds available in the form of various eye formulations for their efficacy. Objective We aimed to investigate the neuroprotective effect of pretreatment with aqueous extract of A . cepa in Ischemia/Reperfusion (I/R) induced retinal injury. Methods Ischemia was induced for 2 h by pterygopalatine artery (PPA) ligation in C57BL/6J mice, followed by reperfusion. The neuroprotective role of oral pretreatment with aqueous extract of A. cepa (300 mg/kg) was analyzed with respect to control and injury only group at 7, 14, and 28 day after the surgery for expression of different genes in the retina by Real-Time PCR. Results Molecular analysis at different time points showed increased expression of BCl-2, GDNF, GFAP, and Brn3b in the retina at 14 and 28 day after A. cepa treatment in comparison to the injury alone group. However, at shorter time point (7th day), the expression of these genes was pronounced in the injury only group in comparison to the injury and pretreated group. Conclusion Pretreatment with aqueous extract of A. cepa may protect from the neuronal damage in I/R-induced retinal injury in mice by altering the expression of neurotrophic factor.

Topical onion juice mitigates the morphological alterations of the cornea in the aged male rats

“…The current study assessed the ability of topical onion juice to ameliorate age-related corneal changes based on histopathological, immunohistochemical and histomorphometric examinations. Despite their limited number, previous experimental studies assessed the effects of onion extracts on corneal changes related to different animal models [18,28], in addition to the effects of onion extracts on the pathological changes of other ocular structures [19,29,30]. Furthermore, several publications indicated that age has major changes on the cornea [31][32][33][34][35].…”

Electrophysiological assessment of retinal functions by ERG in Ischemia/Reperfusion (I/R) Allium cepa pre-treated mice

“…It's a 2 hr occlusion model followed by reperfusion for 3 different time points: 7, 21, and 28-day. Because of the repeated failure (24) and side effects (25) of the available commercial drugs, we wanted to test the alternative approach of testing A. cepa (onion) as the pretreatment strategy to explore if it is able to improve the vision or not. Apart from its many biological benefits in the form of antioxidant, anti-inflammatory, and neuroprotective activity (as shown by previous studies) its common availability makes it an important biological product to be tested for its efficacy (5,10,11).…”

“…The complete experiment was carried according to the GLP guidelines at Neuroscience Research Laboratory (19,20). The A. cepa extract preparation is detailed in our previous publication (21). Different dosages of A. cepa (100 mg/kg, 200 mg/kg, 300 mg/kg) was administered 24 hours prior to the surgery.…”

Policy research into Quality Assessment of Published Data from Medical Institutes Can Increase the Authenticity of Translation

“…Additionally, whether the bench work has been carried out according to the Good Laboratory Practice (GLP) guidelines or not can also be assessed. 6 At the institution level, there are various rules and guidelines for responsible conduct of research. 7 This includes ethical orientation and guidance for researcher, instituting plagiarism check before submission, availability of data in the repository system, supervision of research being conducted, data ownership, data retention, and long-term storage in the form of e-copy besides early reporting of any such misconduct, etc.…”

“…Additionally, whether the bench work has been carried out according to the Good Laboratory Practice (GLP) guidelines or not can also be assessed. 6 …”