Alloantigen-Pulsed Host Dendritic Cells Induce T-Cell Regulation and Prolong Allograft Survival in a Rat Model of Hindlimb Allotransplantation

Kuo, Yur‐Ren; Huang, Cong; Goto, Shigeru; Wang, Chun Ting; Hsu, Li; Lin, Yu; Yang, Kuender D.; Chen, Chao Long; Lee, W. P.Andrew

doi:10.1016/j.jss.2008.05.034

Cited by 27 publications

(25 citation statements)

References 27 publications

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“…Mature DCs express high levels of costimulatory molecules, secrete cytokines including IL-12 and stimulate vigorous Th1 responses. Inversely, immature DCs express low level of costimulatory molecules and induce antigen-specific hyporesponsiveness by triggering T cell apoptosis or inducing T regulatory cells (Morelli and Thomson, 2003;Kuo et al, 2008). Several researches have demonstrated that administration of donor-derived, immature DCs could inhibit the immune responses of recipients to allo-antigens and prolong the survival time of transplanted tissues (Finkelman et al, 1996;Abe et al, 2004;Taner et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Immunosuppressive effects of an ethyl acetate extract from Urtica dentata Hand on skin allograft rejection

Xiang¹,

Hou²,

Xie³

et al. 2009

Journal of Ethnopharmacology

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Section: Discussionmentioning

confidence: 99%

Immunosuppressive effects of an ethyl acetate extract from Urtica dentata Hand on skin allograft rejection

Xiang¹,

Hou²,

Xie³

et al. 2009

Journal of Ethnopharmacology

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“…26 A horseradish peroxidase-diaminobenzidine kit was used (BioGeneX Laboratories, Inc., San Ramon, Calif.) in all experiments. 27 After endogenous peroxidase activity was blocked with 3% hydrogen peroxide for 10 minutes at room temperature, tissue sections were stained with mouse anti-porcine CD25 (Serotec, Oxford, United Kingdom).…”

Section: Immunohistochemical Stainingmentioning

confidence: 99%

Prolongation of Composite Tissue Allotransplant Survival by Treatment with Bone Marrow Mesenchymal Stem Cells Is Correlated with T-Cell Regulation in a Swine Hind-Limb Model

Kuo

Chen

Shih

et al. 2011

Plastic and Reconstructive Surgery

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“…Visualization of specific binding was developed by enzymatic conversion of the chromogenic substrate 3, 3′-DAB into a brown precipitate by HRP. After counterstaining with hematoxylin, donor tissue sections were mounted, cleared, and coverslipped [20].…”

Section: Methodsmentioning

confidence: 99%

“…All images were captured using a Cool CCD camera. The images were analyzed using the Image-Pro Plus image analysis software (Media Cybernetics, Silverbernetics Spring, MD) [20].…”

Section: Methodsmentioning

confidence: 99%

Immunomodulatory Effects of Bone Marrow-Derived Mesenchymal Stem Cells in a Swine Hemi-Facial Allotransplantation Model

et al. 2012

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BackgroundIn this study, we investigated whether the infusion of bone marrow-derived mesenchymal stem cells (MSCs), combined with transient immunosuppressant treatment, could suppress allograft rejection and modulate T-cell regulation in a swine orthotopic hemi-facial composite tissue allotransplantation (CTA) model.Methodology/Principal FindingsOutbred miniature swine underwent hemi-facial allotransplantation (day 0). Group-I (n = 5) consisted of untreated control animals. Group-II (n = 3) animals received MSCs alone (given on days −1, +1, +3, +7, +14, and +21). Group-III (n = 3) animals received CsA (days 0 to +28). Group-IV (n = 5) animals received CsA (days 0 to +28) and MSCs (days −1, +1, +3, +7, +14, and +21). The transplanted face tissue was observed daily for signs of rejection. Biopsies of donor tissues and recipient blood sample were obtained at specified predetermined times (per 2 weeks post-transplant) or at the time of clinically evident rejection. Our results indicated that the MSC-CsA group had significantly prolonged allograft survival compared to the other groups (P<0.001). Histological examination of the MSC-CsA group displayed the lowest degree of rejection in alloskin and lymphoid gland tissues. TNF-α expression in circulating blood revealed significant suppression in the MSC and MSC-CsA treatment groups, as compared to that in controls. IHC staining showed CD45 and IL-6 expression were significantly decreased in MSC-CsA treatment groups compared to controls. The number of CD4+/CD25+ regulatory T-cells and IL-10 expressions in the circulating blood significantly increased in the MSC-CsA group compared to the other groups. IHC staining of alloskin tissue biopsies revealed a significant increase in the numbers of foxp3+T-cells and TGF-β1 positive cells in the MSC-CsA group compared to the other groups.ConclusionsThese results demonstrate that MSCs significantly prolong hemifacial CTA survival. Our data indicate the MSCs did not only suppress inflammation and acute rejection of CTA, but also modulate T-cell regulation and related cytokines expression.

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Alloantigen-Pulsed Host Dendritic Cells Induce T-Cell Regulation and Prolong Allograft Survival in a Rat Model of Hindlimb Allotransplantation

Cited by 27 publications

References 27 publications

Immunosuppressive effects of an ethyl acetate extract from Urtica dentata Hand on skin allograft rejection

Immunosuppressive effects of an ethyl acetate extract from Urtica dentata Hand on skin allograft rejection

Prolongation of Composite Tissue Allotransplant Survival by Treatment with Bone Marrow Mesenchymal Stem Cells Is Correlated with T-Cell Regulation in a Swine Hind-Limb Model

Immunomodulatory Effects of Bone Marrow-Derived Mesenchymal Stem Cells in a Swine Hemi-Facial Allotransplantation Model

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