Allograft aortic valve replacement: Long-term follow-up

Endothelial Cell Preservation at 10°C Minimizes Catalytic Iron, Oxidative Stress, and Cold-Induced Injury

“…Attempts to rectify these defito many factors, including the condition of the donor (15,16,53), the warm ischemia time (5,15), tissue hanciencies by seeding vessels with autologous endothelial cells enhance vessel patency (12,42,55). For aortic valve dling and preservation method (15,33,36), and, following arterial or valve transplants, the allogeneic response allografts, conditions that promote the retention of cellularity and structural integrity of the valve improve valve of the recipient, who may not be immunosuppressed (3,22,24). For the endothelium in particular, preservafunction and durability after implantation (33,57).…”

“…For aortic valve dling and preservation method (15,33,36), and, following arterial or valve transplants, the allogeneic response allografts, conditions that promote the retention of cellularity and structural integrity of the valve improve valve of the recipient, who may not be immunosuppressed (3,22,24). For the endothelium in particular, preservafunction and durability after implantation (33,57). Although the use of cryopreserved tissues is common tion is essential to maintain several crucial functions: to limit vascular permeability and thereby prevent tissue today, reports of spontaneous fracturing (4,37,54) and endothelial cell loss (30,32,36) suggest that improved or edema and hemorrhage; to secrete vasoactive factors that regulate vascular smooth muscle tone and hence alternate methods of preservation are needed.…”

“…However, cold-stored (4°C) arteries and veins have been charactervasculopathy (44,52). Early endothelial dysfunction in transplanted hearts may predict the development of graft ized by poor patency following implantation (10) and 500 ZIEGER AND GUPTA heart valves by reduced viability and inferior durability MATERIALS AND METHODS (33). When minimally processed and implanted within a Cell Culture few hours to a few days following procurement, hypoProliferating bovine aortic endothelial cells (passage thermically stored homovital valves show retention of two) were obtained from Cell Systems (Kirkland, WA) metabolic activity and clinical results that are comparaand maintained at 37°C in an atmosphere of 95% air ble to cryopreserved valves (34,57).…”

In Situ Repair of a Mycotic Suprarenal Aortic Aneurysm Using a Cryopreserved Aortic Homograft and Visceral Debranching in a Patient with Spondylodiscitis and Left Psoas Muscle Abscess

“…Furthermore, in those patients, long-term or even lifelong antibiotic treatment may be inevitable. The favorable long-term results of implanted cryopreserved human cardiac valves 17) have propelled the more common use of cryopreserved aortic homografts within the setting of aortic infections. 7,10,11) There is evidence which indicates that cryopreserved homografts are resistant to bacterial infection, 18,19) and that the immune response of the host may be limited.…”

Impact of current cryopreservation procedures on mechanical and functional properties of human aortic homografts

“…Recently, interest in the use of aortic homografts has reawakend. Excellent results in the use of homograft aortic valves and ascending aortas in endocarditis have encouraged vascular surgeons to use arterial homografts for the in situ repair of vascular infections of the great arteries [27,421. Indications comprise mycotic aneurysms, aorto-esophageal, aortobronchial and aorto-gastrointestinal fistulas and infected prosthetic grafts [l, 4, 12, 16, 17, 19, 26, 28, 32, 37, 38, 39,401.…”