Allosteric Boosting Ofaffinity Sorption

Abstract

Affinity chromatography belongs to the classic preparative and research methods of biochemistry, molecular biology, and biotechnology. This method is based on the application of the affinity of biological molecules to complementary structures. One of the complex-forming participants is immobilized on an insoluble matrix, and the second one is in solution. According to a fairly conventional separation, one of these components contains a binding site that interacts with the complementary grouping of the second component. In this work, a complicated variant of affinity sorption is considered, in which the allosteric site additionally participates in the process of complex formation. Using the example of the activation cascade of the blood clotting system, the possibilities of using such a scheme are considered.