Laparoscopic surgery simulation training system uses human anatomy visual reproduction and force-feedback technology, and uses a variety of medical image data on your computer to establish a virtual environment with vision, hearing, speaking, dynamic, smell, feel, touch. Surgeons conduct surgical training in the virtual environment, which includes suture, knotting, hand-eye coordination, organisational separation, and directional adaptation. In this paper, the issue of simulation-based training with laparoscopic simulation system is dealt with for training of internship surgeons in panhysterectomy. The model is built and the practical training results are analysed and compared for the performance of two groups of internship surgeons under model-based training and conventional training, respectively. The results show that there was no significant difference in total abdominal hysterectomy (TAH) and crossover test for the two groups of internship surgeons, whereas practice test scores increased significantly (p < 0.05), with the group using the model of laparoscopic simulation system scoring higher than the other group. It proves that the laparoscopic simulation system application in gynaecological surgery will help to improve the clinical skills of internship surgeons. The advanced simulation system makes a great contribution to reducing medical accidents, and improves progress of surgery and training.