Aloe vera na criopreservação do sêmen de tambaqui (Colossoma macropomum)

Melo-Maciel, M. A. P.; Leite-Castro, Liliane Veras; Leite, Jordana Sampaio; Oliveira, M. S.; Almeida‐Monteiro, Priscila Silva de; Nunes, J. F.; Salmito‐Vanderley, Carminda Sandra Brito

doi:10.1590/1678-4162-7807

Cited by 8 publications

(3 citation statements)

References 2 publications

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“…Sperm morphologic analysis was performed for fresh and post‐thaw sperm according to Miliorini et al (2011) methodology for this species with modifications in the staining method. The diluted sample was stained with 4% Rose Bengal (3:20; stain: sperm), and two wet preparations per sample were analysed (Melo‐Maciel et al, 2015). For each sample, two slides (a duplicate) were observed under a light microscope (Olympus® CX22LED, Tokyo, Japan) at ×1000 magnification, and the morphology of 200 sperm cells was evaluated.…”

Section: Methodsmentioning

confidence: 99%

Sperm cryopreservation of Prochilodus lineatus throughout the same reproductive season

et al. 2021

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The aim of this study was to determine the effect of seasonality on post‐thaw sperm quality in Prochilodus lineatus. Sperm from 43 males was cryopreserved throughout the reproductive season (November–March). Fresh sperm quality, concentration, volume, seminal plasma pH, osmolality and ion concentration were analysed. Post‐thaw sperm was analysed for its motility rate, velocities, beat‐cross frequency, cell morphology, oxidative stress and fertilization capacity. Prochilodus lineatus fresh sperm yielded high‐quality during the reproductive period (motility >80%). Post‐thaw sperm quality changed throughout the season, with higher motility (63.2%–72.3%) and curvilinear velocity (VCL) (55.9–59.2 µm/s) from December to March. Sperm concentration was negatively correlated with post‐thaw sperm motility and VCL, and positively correlated with seminal plasma Ca2+ concentration, catalase activity (CAT) and reactive oxygen species (ROS). Seminal plasma Ca2+ was positively correlated with ROS and negatively correlated with post‐thaw sperm motility. Post‐thaw sperm CAT and fertility correlated positively. Although post‐thaw sperm quality was reduced in November, the increased CAT was efficient in maintaining its fertilization capacity. In order to face seasonal influence, the optimal period to cryopreserve P. lineatus spermatozoa is from December to March, when sperm exhibits characteristics which make spermatozoa more prone to resist cryodamage.

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Section: Methodsmentioning

confidence: 99%

Sperm cryopreservation of Prochilodus lineatus throughout the same reproductive season

et al. 2021

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“…In the past decade, many studies have developed protocols for tambaqui semen cryopreservation, including evaluation of semen characteristics (volume, motility, sperm count), diluents, cryoprotectants, storage containers, and semen quality pre-and postfreezing, among other aspects, 132,[144][145][146][147]149,[168][169][170][171][172][173][174] and a general protocol for the cryopreservation of tambaqui semen was then established. 148 Cryopreservation has been used to establish germplasm banks to maintain and conserve tambaqui genetic resources.…”

Section: New Technologiesmentioning

confidence: 99%

The farming and husbandry ofColossoma macropomum: From Amazonian waters to sustainable production

Hilsdorf

Hallerman

Valladão

et al. 2021

Reviews in Aquaculture

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Within the rich diversity of South American freshwater fish, Colossoma macropomum (Characiformes: Serrasalmidae), known as tambaqui, cachama or blackfin pacu, can reach 30 kg, is a traditional product in regional fish markets and has drawn the attention of fish farmers since the 1930s. Considerable progress achieved in different fields of aquaculture science has contributed to the growth of tambaqui production.

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“…There are several studies on the reproduction of C. macropomum (Maria, Azevedo, Santos, & Carneiro, ; Martins et al, ; Varela Jr et al, ), some of which even examined sperm quality using ( casa ) in fresh sperm (Gallego et al, ; Maria et al, ; Melo‐Maciel, Nunes et al, ; Pires et al, ) and cryopreserved sperm (Gallego et al, ; Melo‐Maciel, Leite‐Castro et al, ). However, there are no reports on evaluation using casa software on the quality of cooled semen of C. macropomum .…”

Section: Introductionmentioning

confidence: 99%

Sperm quality of Colossoma macropomum after room‐temperature and cold storage

Pires

Sanches

Romagosa³

et al. 2019

J Appl Ichthyol

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Summary The objective of this study was to evaluate the effects of cold and room‐temperature storage on the quality of Colossoma macropomum sperm. The experiment was carried out in December (end of Spring), in Nova Mutum‐MT, Brazil, involving nine C. macropomum males (4 years old; 6.4 ± 1.5 kg average weight). The fish were selected and transferred to masonry tanks (4 m3) in a laboratory (water renewal rate: 10 L/s; average water temperature: 28°C). Subsequently, reproduction was induced using 2.5 mg of crude carp pituitary extract/kg and the semen was harvested 240 degree hours after hormonal induction. The following sperm characteristics were analyzed every 5 hr using ImageJ/casa software: total motility (MOT), curvilinear velocity (VCL), average path velocity (VAP), straight‐line velocity (VSL), straightness of sperm path (STR), wobble (WOB), progressive motility (PROG), beat cross frequency (BCF) and total number of spermatozoa (NSPZ). A fresh sample of semen from each animal was kept at room temperature (25.3 ± 1.2°C). For analysis of cooled semen, syringes were kept in cooling boxes at an average temperature of 16.9 ± 2.1°C. The reduction (p < 0.05) of MOT in semen kept at room temperature occurred at 10 hr (13.95%); in cooled semen, however, MOT declined at 15 hr (76.87%). At 15 hr, there was practically no MOT in the semen kept at room temperature (0.20%), whereas in the cooled semen this situation was observed only at 35 hr (2.91%) The MOT of cooled sperm was higher (p < 0.05) at all times (except zero time), compared with the semen maintained at room temperature. At 15 hr, the cooled spermatozoa showed higher (p < 0.05) VCL (142.18 μm/s) and BCF (29.72 Hz) than those maintained at room temperature (VCL: 51.18 μm/s; BCF: 19.57 Hz). After 15 hr, only the cooled sperm showed quality. In conclusion, semen cooling allows for extending the viability of C. macropomum spermatozoa from 5 to 10 hr without compromising their quality in most characteristics. At 15 and 25 hr of cooling, sperm viability is still observed, though with decreased quality.

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Aloe vera na criopreservação do sêmen de tambaqui (Colossoma macropomum)

Cited by 8 publications

References 2 publications

Sperm cryopreservation of Prochilodus lineatus throughout the same reproductive season

Sperm cryopreservation of Prochilodus lineatus throughout the same reproductive season

The farming and husbandry ofColossoma macropomum: From Amazonian waters to sustainable production

Sperm quality of Colossoma macropomum after room‐temperature and cold storage

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