Alpha/Beta and Gamma Interferons Are Induced by Infection with Noncytopathic Bovine Viral Diarrhea Virus In Vivo

Charleston, Bryan; Brackenbury, Louise S.; Carr, B. Veronica; Fray, Martin; Hope, Jayne; Howard, Chris; Morrison, W. Ivan

doi:10.1128/jvi.76.2.923-927.2002

Cited by 60 publications

(37 citation statements)

References 22 publications

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“…The levels of IFN found in pig plasma from CSFVinfected animals are significantly higher than those seen in serum from BVDV-infected cattle (17,43). Acid treatment of the porcine plasma samples confirmed the presence of acid labile IFN, which comprised up to 70% of the total IFN produced (Fig.…”

Section: Porcine Plasma Contains Acid-labile Ifnmentioning

confidence: 66%

“…Despite the ability of pestiviruses to block type I IFN in the majority of host target cells, IFN can be detected in serum samples from cattle early postinfection with ncp BVDV and following CSFV infection of pigs. Elevated serum levels of type I IFN were observed for ∼6 d by Charleston et al (17) following infection of postnatal cattle with ncp BVDV.…”

mentioning

confidence: 99%

“…Type I IFN in the serum of animals infected with BVDV has been previously reported (17). Following our observation that type III IFN is the major constituent of the total IFN that is produced by enriched pDC populations in response to infection with both recombinant and wild-type BVDV, we decided to examine the proportion of type III IFN generated in vivo in response to each of these viruses using the Mx-CAT reporter assay (32).…”

Section: Analysis Of Serum Ifn From Bvdv-infected Animalsmentioning

confidence: 99%

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Type I and III IFNs Produced by Plasmacytoid Dendritic Cells in Response to a Member of theFlaviviridaeSuppress Cellular Immune Responses

Reid

Juleff

Windsor

et al. 2016

The Journal of Immunology

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The pestivirus noncytopathic bovine viral diarrhea virus (BVDV) can suppress IFN production in the majority of cell types in vitro. However, IFN is detectable in serum during acute infection in vivo for ∼5–7 d, which correlates with a period of leucopoenia and immunosuppression. In this study, we demonstrate that a highly enriched population of bovine plasmacytoid dendritic cells (DCs) produced IFN in response to BVDV in vitro. We further show that the majority of the IFN produced in response to infection both in vitro and in vivo is type III IFN and acid labile. Further, we show IL-28B (IFN-λ3) mRNA is induced in this cell population in vitro. Supernatant from plasmacytoid DCs harvested postinfection with BVDV or recombinant bovine IFN-α or human IL-28B significantly reduced CD4+ T cell proliferation induced by tubercle bacillus Ag 85–stimulated monocyte-derived DCs. Furthermore, these IFNs induced IFN-stimulated gene expression predominantly in monocyte-derived DCs. IFN-treated immature DCs derived from murine bone marrow also had a reduced capacity to stimulate T cell proliferative responses to tubercle bacillus Ag 85. Immature DCs derived from either source had a reduced capacity for Ag uptake following IFN treatment that is dose dependent. Immunosuppression is a feature of a number of pestivirus infections; our studies suggest type III IFN production plays a key role in the pathogenesis of this family of viruses. Overall, in a natural host, we have demonstrated a link between the induction of type I and III IFN after acute viral infection and transient immunosuppression.

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Section: Porcine Plasma Contains Acid-labile Ifnmentioning

confidence: 66%

mentioning

confidence: 99%

Section: Analysis Of Serum Ifn From Bvdv-infected Animalsmentioning

confidence: 99%

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Type I and III IFNs Produced by Plasmacytoid Dendritic Cells in Response to a Member of theFlaviviridaeSuppress Cellular Immune Responses

Reid

Juleff

Windsor

et al. 2016

The Journal of Immunology

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“…Transient lymphopenia and immunosuppression characterized by loss of T-cell responses is also evident in animals infected with BVDV (Charleston et al, , 2002, but whether the mechanism of this is similar to that seen with other viruses is not known. DCs transport measles virus to the lymph node and measles virus infection of DCs in vivo results in a reduced ability to stimulate allogeneic T cells, partly due to a CD40L signalling defect and reduced IL-12 synthesis and possibly also due to the effect of viral protein expressed on the surface of DCs giving a negative signal to the T cell.…”

Section: Discussionmentioning

confidence: 99%

Differential effects of bovine viral diarrhoea virus on monocytes and dendritic cells

Glew

Carr

Brackenbury

et al. 2003

Journal of General Virology

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Various pathogens have been shown to infect antigen-presenting cells and affect their capacity to interact with and stimulate T-cell responses. We have used an antigenically identical pair of noncytopathic (ncp) and cytopathic (cp) bovine viral diarrhoea virus (BVDV) isolates to determine how the two biotypes affect monocyte and dendritic cell (DC) function. We have shown that monocytes and DCs are both susceptible to infection with ncp BVDV and cp BVDV in vitro. In addition, monocytes infected with ncp BVDV were compromised in their ability to stimulate allogeneic and memory CD4+ T cell responses, but DCs were not affected. This was not due to down-regulation of a number of recognized co-stimulatory molecules including CD80, CD86 and CD40. Striking differences in the response of the two cell types to infection with cytopathic virus were seen. Dendritic cells were not susceptible to the cytopathic effect caused by cp BVDV, whereas monocytes were killed. Analysis of interferon (IFN)-a/b production showed similar levels in monocytes and DCs exposed to cp BVDV, but none was detected in cells exposed to ncp BVDV. We conclude that the prevention of cell death in DCs is not associated with enhanced production of IFN-a/b, as proposed for influenza virus, but is by a distinct mechanism.

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“…Infection of post-natal cattle with a non cytopathic strain of bovine viral diarrhea virus (ncpBVDV) resulted in elevated circulating IFN-α/β [15]. Although the bovine IFN-producing cells share some features with porcine NIPC (they are found in lymph node draining the site of infection and they are distinct from BVDV infected cells), their phenotype does not match the one expected for typical plasmacytoid DC [12].…”

Section: Discovery Of Nipc-pdcmentioning

confidence: 86%

Antigen presenting cells in mucosal sites of veterinary species

Haverson

Riffault

2006

Vet. Res.

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Alpha/Beta and Gamma Interferons Are Induced by Infection with Noncytopathic Bovine Viral Diarrhea Virus In Vivo

Cited by 60 publications

References 22 publications

Type I and III IFNs Produced by Plasmacytoid Dendritic Cells in Response to a Member of theFlaviviridaeSuppress Cellular Immune Responses

Type I and III IFNs Produced by Plasmacytoid Dendritic Cells in Response to a Member of theFlaviviridaeSuppress Cellular Immune Responses

Differential effects of bovine viral diarrhoea virus on monocytes and dendritic cells

Antigen presenting cells in mucosal sites of veterinary species

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