Alpha-Tocopherol Improves Sperm Quality by Regulate Intracellular Ca2+ Intensity (Influx/Efflux) of Simmental Bull Cattle Sperm

Abstract

<jats:p>Background: The effects of α-tocopherol on intracellular Ca2+ intensity in semen cryopreservation by regulate intracellular Ca2+ intensity have not been reported yet. Objective: The research was conducted to evaluate the effect of supplementation α-tocopherol into egg yolk skim milk extender on sperm quality and intracellular Ca2+ intensity. Methods: Semen samples were collected and supplemented with respectively 0mM (P0); 0.5mM (P1); 1mM (P2); 1.5mM (P3) and 2mM (P4) α-tocopherol in extender before cryopreservation processes. Post-thawing sperm was evaluated for motility, viability, and abnormality using Phase Contrast Microscope (200x) with eosin-nigrosine staining, and intracellular Ca2+ intensity of the best result dose was evaluated using Confocal Laser Scan Microscope (400x) with Fluo-3 Staining. Results: The results showed there was a significant difference (P≤0.05) in sperm motility and viability between P0; P1 with P2; P3; P4. The Motility and viability between groups P0; P1 and P3; P4 showed no significant difference (P≥0.05), while P2 with P3; P4 showed significant difference (P≤0.05). There was a significant difference (P≤0.05) in sperm abnormality of P0; P1 with P2; P3; P4. The abnormality between P0; P1 and P2; P3 showed no significant difference (P≥ 0.05), while P2; P3 showed a significant difference with P4 (P≤0.05). The best result in sperm quality was supplementation with 1.5mM α-tocopherol. Ca2+ intracellular intensity: 142.76 ± 21.8 au (P0) and 176.06 ± 61.43 au (P3). Conclusions: It was concluded that 1.5mM α-tocopherol is the best dose to improve sperm quality by regulating intracellular Ca2+ intensity on Simmental bull cattle.</jats:p>

Protection of Myrmecodia Extract in Tris Diluent on PO Bull Sperm Quality During Freezing

“…Research on Entada abyssinica extract containing tannin can increase post-thawing progressive motility and maintain plasma membrane integrity (Sobeh et al, 2020). Adding 1.5mM tocopherol maintained increased NCDs and sperm viability (Ratnani et al, 2020) be carried out regarding the use of tannin in myrmecodia extract on sperm quality. The ability to survive sperm after freezing can be seen from the sperm motility recovery rate (RR) value in Table 5.…”

Evaluation Deoxyribonucleic acid (DNA) fragmentation of local Indonesian cattle frozen sperm using Halomax method

“…Large seasonal oscillations in the reproduction of tropical bulls have been linked to changes in nutrition, habitat, phenotype, and possibly photoperiod (Ratnani et al 2020). But seasonal changes in herd fertility are rarely caused by bull-related variables.…”

“…Additionally, tropical countries are characterized by maritime geography and are typically composed of numerous small islands that are widely spaced. Thus, the beef cattle industry's ability to deal with this challenge will greatly benefit from well-managed artificial insemination with frozen semen (Ratnani et al 2020). Therefore, to further enhance sperm quality, a beneficial solution in the form of extra active substances is needed (Bustani and Baiee 2021).…”

Comparison various level ascorbic acid and lycopene additions in semen diluent enhanced sperm quality of Sapudi ram

“…In addition, tropical nations like Indonesia are typically separated into multiple small archipelagos spread across the ocean. Effective management of artificial insemination with frozen semen is crucial for the small ruminant sector to become more resilient to increased animal productivity spread evenly throughout the country (5).…”