Alpinia oxyphylla Miq. extract changes miRNA expression profiles in db-/db- mouse kidney

Du, Guankui; Xiao, Mochao; Zhang, Xuezi; Wen, Ming; Pang, Chao; Jiang, Shangfei; Sheng-gang, Sang; Xie, Yiqiang

doi:10.1186/s40659-017-0111-1

Cited by 21 publications

(19 citation statements)

References 45 publications

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“…Previous studies have shown that A. oxyphylla can promote the migration and proliferation of human adipose tissue-derived stromal cells [ 21 , 22 ]. In our previous study, we found that A. oxyphylla extract (AOE) exhibits an antioxidant and anti-T2DM capacity [ 23 , 24 ]. However, the mechanism of AOE-mediated anti-T2DM function remains largely unknown.…”

Section: Introductionmentioning

confidence: 99%

Alpinia oxyphyllaMiq. Extract Prevents Diabetes in Mice by Modulating Gut Microbiota

Xie

Xiao

et al. 2018

Journal of Diabetes Research

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Recently, the role of gut microbiota in the development of obesity and type 2 diabetes mellitus (T2DM) has been highlighted. We performed an 8-week administration protocol on T2DM (C57BL/6J db-/db-) mice and fecal samples were collected. Comparisons of fecal bacterial communities were performed between db-/db- mice and normal mice (DB/DB) and between the db-/db mice treated and untreated with AOE using next-generation sequencing technology. Our results showed that the db-/db-AOE group had improved glycemic control and renal function compared with the db-/db-H2O group. Compared with the db-/db-H2O group, AOE administration resulted in significantly increased ratio of Bacteroidetes-to-Firmicutes in db-/db- mice. In addition, the abundance of Akkermansia was significantly increased, while Helicobacter was significantly suppressed in the db-/db-AOE group compared with the db-/db-H2O group. Our data suggest that AOE treatment decreased blood glucose levels and significantly reduced damage of renal pathology in the T2DM mice by modulating gut microbiota composition.

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Section: Introductionmentioning

confidence: 99%

Alpinia oxyphyllaMiq. Extract Prevents Diabetes in Mice by Modulating Gut Microbiota

Xie

Xiao

et al. 2018

Journal of Diabetes Research

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“…The huge amount of NKT and simple extraction mean it is easily obtained and has been applied as a functional ingredient of health foods or medical agents to prevent disorders or diseases. More interestingly, the extracts of A. oxyphylla have been used to improve urinary and renal functions, such as polyuria, hyperuricemia [8], and diabetic nephropathy in animal models [10,49]. These findings provided empirical evidence to speculate a possible excellent preventive candidate such as NKT to mitigate the progression of kidney diseases.…”

Section: Discussionmentioning

confidence: 97%

“…It has also been broadly used as the traditional medicine for the treatment of diarrhea, ulceration, and enuresis [7]. Its fruit extracts revealed an anti-hyperuricemic effect in the kidney [8], hepatoprotective function against CCl 4 -induced oxidative damage in vitro and in vivo [9], and anti-diabetic action [10]. Recent pharmacological evidence indicated that one of the important active ingredients of A. oxyphyllae, nootkatone (NKT), exhibited excellent protective and inhibitory effects on inflammation and apoptosis in neuronal cells [11], displayed improved properties against particle-induced lung injury in mice, and also prevented fibrotic actions and inflammatory response in a liver injury murine model [12].…”

Section: Introductionmentioning

confidence: 99%

Protective Effects of Nootkatone on Renal Inflammation, Apoptosis, and Fibrosis in a Unilateral Ureteral Obstructive Mouse Model

et al. 2021

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Nootkatone is one of the major active ingredients of Alpiniae oxyphyllae, which has been used as both food and medicinal plants for the treatment of diarrhea, ulceration, and enuresis. In this study, we aimed to investigate whether nootkatone treatment ameliorated the progression of chronic kidney diseases (CKD) and clarified its underlying mechanisms in an obstructive nephropathy (unilateral ureteral obstructive; UUO) mouse model. Our results revealed that nootkatone treatment preventively decreased the pathological changes and significantly mitigated the collagen deposition as well as the protein expression of fibrotic markers. Nootkatone could also alleviate oxidative stress-induced injury, inflammatory cell infiltration, and renal cell apoptotic death in the kidneys of UUO mice. These results demonstrated for the first time that nootkatone protected against the progression of CKD in a UUO mouse model. It may serve as a potential therapeutic candidate for CKD intervention.

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“…Firstly, db/db mice, as the model of type 2 DN with significant hyperglycemia and insulin resistance, are the excellent obesity model with the leptin receptor gene mutation, whose renal lesions is not severe (Kitada et al, 2016). Secondly, wild type mice, instead of db/m, was used as a normal control, since it was reported that wild type mice better reflected the normal state of miRNA expression, and wild type littermates were reported to be superior to db/m as normal controls when performing miRNA sequence analysis (Du et al, 2017). Thirdly, QDTS with multi-components might offer reno-protective effects by modulating multi-targets, which makes much difficulty to support the causal relationship between nutrient-sensing signaling molecules and kidney damage directly, since it is difficult to demonstrate a causal relationship by only blocking one pathway.…”

Section: Discussionmentioning

confidence: 99%

QiDiTangShen Granules Activate Renal Nutrient-Sensing Associated Autophagy in db/db Mice

Wang

Ajay

et al. 2019

Front. Physiol.

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QiDiTangShen granules (QDTS) have been proven to reduce the proteinuria in patients with diabetic nephropathy (DN) effectively. The present study was aimed to investigate the mechanism underlying QDTS’s renoprotection. The main components of QDTS were identified by ultra-high liquid chromatography-tandem mass spectrometry and pharmacological databases, among which active components were screened by oral bioavailability and drug-likeness. Their regulation on autophagy-related nutrient-sensing signal molecules (AMPK, SIRT1, and mTOR) was retrieved and analyzed through the Pubmed database. Then, db/db mice were randomly divided into three groups (model control, valsartan and QDTS), and given intragastric administration for 12 weeks, separately. Fasting and random blood glucose, body weight, urinary albumin excretion (UAE) and injury markers of liver and kidney were investigated to evaluate the effects and safety. Renal histological lesions were assessed, and the expressions of proteins related to nutrient-sensing signals and autophagy were investigated. Thirteen active components were screened from 78 components identified. Over half the components had already been reported to improve nutrient-sensing signals. QDTS significantly reduced UAE, ameliorated mesangial matrix deposition, alleviate the expression of protein and mRNA of TGF-β, α-SMA, and Col I, as well as improved the quality of mitochondria and the number of autophagic vesicles of renal tubular cells although the blood glucose was not decreased in db/db mice. Compared to the db/db group, the expression of the autophagy-inducible protein (Atg14 and Beclin1) and microtubule-associated protein 1 light chain 3-II (LC3-II) were up-regulated, autophagic substrate transporter p62 was down-regulated in QDTS group. It was also found that the expression of SIRT1 and the proportion of p-AMPK (thr172)/AMPK were increased, while the p-mTOR (ser2448)/mTOR ratio was decreased after QDTS treatment in db/db mice, which was consistent with the effect of its active ingredients on the nutrient-sensing signal pathway as reported previously. Therefore, QDTS may prevent the progression of DN by offering the anti-fibrotic effect. The renoprotection is probably attributable to the regulation of nutrient-sensing signal pathways, which activates autophagy.

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Alpinia oxyphylla Miq. extract changes miRNA expression profiles in db-/db- mouse kidney

Cited by 21 publications

References 45 publications

Alpinia oxyphyllaMiq. Extract Prevents Diabetes in Mice by Modulating Gut Microbiota

Alpinia oxyphyllaMiq. Extract Prevents Diabetes in Mice by Modulating Gut Microbiota

Protective Effects of Nootkatone on Renal Inflammation, Apoptosis, and Fibrosis in a Unilateral Ureteral Obstructive Mouse Model

QiDiTangShen Granules Activate Renal Nutrient-Sensing Associated Autophagy in db/db Mice

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