Alteration Expression of Bax, Bcl-2 and VDAC1 Genes in Oligozoospermic and Fertile Subjects

Almasi‐Hashiani, Amir; Yanwirasti,; Asmarinah,; Oenzil, Fadil

doi:10.3923/pjbs.2016.71.76

Cited by 4 publications

(3 citation statements)

References 9 publications

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“…Meanwhile, both voltage-dependent anion channel-1 (VDAC1) and −2 (VDAC2) are capable of forming stable and highly conductive voltage-gated channels, which provide the major passage for the transportation of ions and metabolites across the mitochondrial outer membrane [ 44 ]. Moreover, the abundances of VDAC1 and VDAC2 are closely related to spermatogenesis [ 45 , 46 ].…”

Section: Discussionmentioning

confidence: 99%

Identification of postnatal development dependent genes and proteins in porcine epididymis

Fang,

Li,

Pang

et al. 2023

BMC Genomics

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Background The epididymis is a highly regionalized tubular organ possesses vectorial functions of sperm concentration, maturation, transport, and storage. The epididymis-expressed genes and proteins are characterized by regional and developmental dependent pattern. However, a systematic and comprehensive insight into the postnatal development dependent changes in gene and protein expressions of porcine epididymis is still lacking. Here, the RNA and protein of epididymis of Duroc pigs at different postnatal development stages were extracted by using commercial RNeasy Midi kit and extraction buffer (7 M Urea, 2 M thiourea, 3% CHAPS, and 1 mM PMSF) combined with sonication, respectively, which were further subjected to transcriptomic and proteomic profiling. Results Transcriptome analysis indicated that 198 and 163 differentially expressed genes (DEGs) were continuously up-regulated and down-regulated along with postnatal development stage changes, respectively. Most of the up-regulated DEGs linked to functions of endoplasmic reticulum and lysosome, while the down-regulated DEGs mainly related to molecular process of extracellular matrix. Moreover, the following key genes INSIG1, PGRMC1, NPC2, GBA, MMP2, MMP14, SFRP1, ELN, WNT-2, COL3A1, and SPARC were highlighted. A total of 49 differentially expressed proteins (DEPs) corresponding to postnatal development stages changes were uncovered by the proteome analysis. Several key proteins ACSL3 and ACADM, VDAC1 and VDAC2, and KNG1, SERPINB1, C3, and TF implicated in fatty acid metabolism, voltage-gated ion channel assembly, and apoptotic and immune processes were emphasized. In the integrative network, the key genes and proteins formed different clusters and showed strong interactions. Additionally, NPC2, COL3A1, C3, and VDAC1 are located at the hub position in each cluster. Conclusions The identified postnatal development dependent genes and proteins in the present study will pave the way for shedding light on the molecular basis of porcine epididymis functions and are useful for further studies on the specific regulation mechanisms responsible for epididymal sperm maturation.

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Section: Discussionmentioning

confidence: 99%

Identification of postnatal development dependent genes and proteins in porcine epididymis

Fang,

Li,

Pang

et al. 2023

BMC Genomics

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“…20,21 Likewise, Amir et al reported that the altered expressions of pro-apoptotic Bax and voltage-dependent anion channel 1, and anti-apoptotic Bcl-2 are linked to oligozoospermia. 22 However, the molecular mechanism underlying apoptosis in spermatogenesis has not been completely elucidated and requires further analysis.…”

Section: Discussionmentioning

confidence: 99%

Homo Sapiens (Human) microRNA 187 Expression Is Dysregulated in Testis of Non-obstructive Azoospermic Men

Piryaei

Mozdarani

Gilani

et al. 2020

Int J Basic Sci Med

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Introduction: MiRNA expression alterations are closely related to growth, proliferation, and cell development. In addition, miR-187 has been found to regulate cellular proliferation. Further, spermatogenesis is a highly complicated process which is regulated through several genes targeted by miRNAs. However, no study has completely addressed the etiology of spermatogenic impairments. Accordingly, the present study examined the expression pattern of Homo sapiens (human) microRNA (hsa-mir-187) in the testicular biopsies of infertile men with non-obstructive azoospermia (NOA) and Sertoli cell-only syndrome (SCOS) subtype in comparison with those with obstructive azoospermia (OA). Methods: Quantitative reverse‐transcription polymerase chain reaction analysis was performed to assess the expression level of hsa-mir-187 using the ΔΔCt method. Results: Our data indicated that hsa-mir-187 was significantly up-regulated in the testis of the SCOS group (P˂ 0.05) in comparison with the OA group. Conclusion: In general, the findings suggest that the differential expression of hsa-mir-187 is linked with male infertility and shed more light on molecular defects that underlie spermatogenic impairments.

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“…Overexpression of VDAC1 enhanced oxidative stress-induced apoptosis in cerebellar granule neurons (Liao et al, 2015). Amir and colleagues conducted a clinical trial and found that the mRNA levels of Bax and VDAC1 were significantly elevated in spermatozoa from oligozoospermia patients compared to that in the fertile donors (Amir et al, 2016). Deletion of cystic fibrosis transmembrane conductance regulator (CFTR), an important regulator of male fertility, slightly increased VDAC1 expression in mice testis.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of VDAC1 prevents oxidative stress and apoptosis induced by bisphenol A in spermatogonia via AMPK/mTOR signaling pathway

Wang

Liu

et al. 2023

J. Toxicol. Sci.

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Bisphenol A (BPA), one of the main components of industrial products, is clinically associated with the increased male infertility rate. However, the underlying molecular mechanism of the BPA-resulted reproductive toxicity is not fully elucidated. Voltage-dependent anion channel 1 (VDAC1) is a pore protein and located at the outer mitochondrial membrane. As a mitochondrial gatekeeper, VDAC1 controls the release of reactive oxygen species (ROS) and the metabolic and energetic functions of mitochondria, and serves as a critical player in mitochondrial-mediated apoptosis. Herein, we explored the role of VDAC1 in BPA-induced apoptosis of spermatogonia. The results showed that BPA increased spermatogonia cell line GC-1 spg cell apoptosis and intracellular ROS level, and suppressed AMPK/mTOR signaling pathway at a dose of 80 μM for 48 hr. Lentivirus-mediated short hairpin RNA targeting VDAC1 (Lv-shVDAC1) silenced VDAC1 expression and enhanced BPA-restricted cell viability. Knockdown of VDAC1 inhibited the apoptosis of BPA-treated GC-1 spg cells determined by with changes of the expressions of pro-apoptotic and anti-apoptotic proteins. Knockdown of VDAC1 also alleviated the BPA-triggered intracellular ROS generation and oxidative stress. Moreover, silence of VDAC1 increased AMPKα1/2 phosphorylation and suppressed mTOR phosphorylation under BPA exposure. Dorsomorphin, an AMPK inhibitor, partially abolished the effects of VDAC1 gene silencing on BPA-stimulated GC-1 spg cells. In conclusion, inhibition of VDAC1 attenuated the BPA-induced oxidative stress and apoptosis and promoted the cell viability in spermatogonia through modulating AMPK/mTOR signaling pathway.

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Alteration Expression of Bax, Bcl-2 and VDAC1 Genes in Oligozoospermic and Fertile Subjects

Cited by 4 publications

References 9 publications

Identification of postnatal development dependent genes and proteins in porcine epididymis

Identification of postnatal development dependent genes and proteins in porcine epididymis

Homo Sapiens (Human) microRNA 187 Expression Is Dysregulated in Testis of Non-obstructive Azoospermic Men

Inhibition of VDAC1 prevents oxidative stress and apoptosis induced by bisphenol A in spermatogonia via AMPK/mTOR signaling pathway

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