Alteration in biochemical parameters in the brain of transgenic Drosophila melanogaster model of Parkinson’s disease exposed to apigenin

Siddique, Yasir Hasan; Jyoti, Smita

doi:10.1016/j.imr.2017.04.003

Cited by 41 publications

(14 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is often used in humans for Alzheimer's disease treatments. Flies that were reared on apigenin in their diet showed an increase in lifespan, glutathione, and dopamine content, as well as reduced oxidative stress and apoptosis in a transgenic Drosophila model of Parkinson's disease (Siddique and Jyoti, 2017). Treatment with caffeic acid alleviates oxidative stress induced neurotoxicity in cells and Drosophila models (Wu et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Anise Hyssop Agastache foeniculum Increases Lifespan, Stress Resistance, and Metabolism by Affecting Free Radical Processes in Drosophila

et al. 2020

View full text Add to dashboard Cite

Anise hyssop, Agastache foeniculum, is a widely used medicinal herb with known antioxidant properties. We studied how dietary supplementation with dried A. foeniculum leaf powder affected physiological and metabolic traits as well as activities of antioxidant enzymes and markers of oxidative stress in Drosophila melanogaster. Dietary hyssop extended the lifespan in a sex and genotype independent manner over a broad range of concentrations up to 30 mg/ml. Dietary supplementation with the herb significantly increased fecundity, resistance to oxidative stress and starvation. Higher transcript levels of Drosophila insulin-like peptide (dilp2) and decreased dilp3 and dilp6 transcripts together with increased levels of glycogen and triacylglycerols support an alteration of insulin signaling by the plant extract. Increased enzymatic activities of superoxide dismutase and aconitase as well as elevated protein and low molecular mass thiols also supported an alteration of free radical process in flies treated with dietary A. foeniculum leaf powder. Thus, physiological and metabolic traits as well as free radical processed may be affected by active compounds detected in extracts of anise hyssop leaves and contribute to the increased lifespan and reproductive (egg-laying) activity observed.

show abstract

Section: Discussionmentioning

confidence: 99%

Anise Hyssop Agastache foeniculum Increases Lifespan, Stress Resistance, and Metabolism by Affecting Free Radical Processes in Drosophila

et al. 2020

View full text Add to dashboard Cite

show abstract

“…the progressive loss of dopaminergic neurons [34]. Various epidemiological and biochemical studies have recently identified promising compounds that may elicit neuroprotection in PD [24,28,29]. Due to ethical reasons the experiments cannot be performed directly on human, hence model organisms such as mice, fruit flies and worms have been developed in order to understand the biology of the disease as well as pharmacological interventions [18].…”

Section: Introductionmentioning

confidence: 99%

Effect of capsaicin on the oxidative stress and dopamine content in the transgenic Drosophila model of Parkinson’s disease

Siddique

Naz

Jyoti

2018

Acta Biologica Hungarica

Self Cite

View full text Add to dashboard Cite

In the present study the effect of capsaicin was studied on PD model flies expressing human alpha synuclein. First the potential of scavenging superoxide anion and free radicals by capsaicin at doses of 20, 40, 80 and 100 μM was estimated. The PD flies were allowed to feed separately on the diet containg 20, 40, 80 and 100 μM of capsaicin, respectively, for 24 days. After 24 days of exposure, fly head homogenate was prepared from each group and was used to estimate glutathione (GSH), protein carbonyl (PC), dopamine content, lipid peroxidation (LPO), glutathione-S-transferase (GST) and monoamine oxidase (MAO) activity. A dose dependent significant increase in the potential of scavenging superoxide anions and free radicals by capsaicin was observed for the doses of 20, 40, 80 and 100 μM. The exposure of capsaicin not only significantly increased the GSH (max. by 1.37-fold), and dopamine (max. by 1.56-fold) content but also reduced LPO (max. by 1.8-fold), GST (max. by 1.26-fold), MAO activities (max. by 1.60-fold) and PC content (max. by 1.95-fold), compared to unexposed PD flies (p < 0.05). The results suggest the protective role of capsaicin against the PD symptoms.

show abstract

“…Reduced life span of fly has been linked with various neurodegenerative disorders [33]. Earlier studies on natural plant products/extract have also shown an increase in the life span of fruit flies expressing the symptoms of neurodegenerative disorder [15, 23, 33, 34, 35]. The exposure of PD flies to MB also prevents the loss in the activity of flies as is evident from the average activity pattern recorded by Drosophila Activity Monitor (DAM).…”

Section: Discussionmentioning

confidence: 99%

Effect of Majun Baladur on life span, climbing ability, oxidative stress and dopaminergic neurons in the transgenic Drosophila model of Parkinson's disease

Siddique

Naz

Rahul

et al. 2019

Heliyon

Self Cite

View full text Add to dashboard Cite

The effect of a poly herbal drug Majun Baladur (MB) was studied on the transgenic Drosophila melanogaster expressing human alpha synuclein in the neurons (PD flies). The equivalents of recommended dose for human were established for 20 g of fly food i.e. 0.0014, 0.0028, 0.0042 and 0.0056 g per 20 g of diet. The PD flies were allowed to feed on it for 24 days before performing the assays. The exposure to MB increased the life span and improves the activity of PD flies. The PD flies exposed to 0.0014, 0.0028, 0.042 and 0.0056 g of MB showed a dose dependent significant delay of 1.47, 1.88, 2.52 and 3.05 folds in the climbing ability compared to unexposed PD flies. A dose dependent significant decrease of 1.38, 1.45, 1.48 and 1.65 folds in TBARS; 1.08, 1.11, 1.17 and 1.20 folds in the GST activity; 1.20, 1.28, 1.39 and 1.52 folds in the PC content; 1.43, 1.53, 1.65 and 1.79 folds in the Caspase-9 activity; 1.21, 1.31, 1.53 and 1.64 folds in the activity of Caspase-3 and 1.24, 1.42, 1.50 and 1.79 folds in the activity of catalase; 1.50, 1.63, 1.88 and 2.06 folds in the activity of SOD in PD flies exposed to 0.0014, 0.0028, 0.042 and 0.0056 g of MB, respectively. A significant dose dependent increase of 1.20, 1.29, 1.33 and 1.44 folds in as NPSH content was observed in PD flies exposed to 0.0014, 0.0028, 0.042 and 0.0056 g of MB, respectively. The exposure to MB protects the loss of dopaminergic neurons as is evident by immunohistochemistry. It is concluded that MB is potent in reducing the PD symptoms being mimicked in the transgenic flies.

show abstract

Alteration in biochemical parameters in the brain of transgenic Drosophila melanogaster model of Parkinson’s disease exposed to apigenin

Cited by 41 publications

References 67 publications

Anise Hyssop Agastache foeniculum Increases Lifespan, Stress Resistance, and Metabolism by Affecting Free Radical Processes in Drosophila

Anise Hyssop Agastache foeniculum Increases Lifespan, Stress Resistance, and Metabolism by Affecting Free Radical Processes in Drosophila

Effect of capsaicin on the oxidative stress and dopamine content in the transgenic Drosophila model of Parkinson’s disease

Effect of Majun Baladur on life span, climbing ability, oxidative stress and dopaminergic neurons in the transgenic Drosophila model of Parkinson's disease

Contact Info

Product

Resources

About