Alteration of BMP‐4 and <i>Runx2</i> expression patterns in mouse temporomandibular joint after ovariectomy

Hj, Min; Mj, Lee; Jy, Kim; Cho, Sung Won; Hd, Park; Liu, Si; Kim, Hyung Jun; Jung, Han‐Sung

doi:10.1111/j.1601-0825.2006.01270.x

Cited by 15 publications

(13 citation statements)

References 31 publications

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“…A previous study 36 reported intense and moderate RANKL staining on osteoblasts and osteoclasts. Positive BSP staining was also previously reported 12 during the first week after ovariectomy and OCC expression was clearly changed by ovariectomy 37 .…”

Section: Discussionmentioning

confidence: 62%

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Effects of estrogen deficiency combined with chronic alcohol consumption on rat mandibular condyle

Salgado¹,

Marchini²,

Tera³

et al. 2015

Braz. J. Oral Sci.

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Estrogen deficiency and chronic alcohol consumption may have a synergistic and deleterious effect on bone tissue. Aim: To investigate the effects of estrogen deficiency associated with chronic alcohol consumption on the mandibular condyle in rats. Methods: Fifty-four female rats were first divided equally into two groups: ovariectomized (Ovx) and simulated ovariectomy (Sham). One month after the surgeries, these groups were equally sub-divided according to their dietary treatment: G1: Sham/ad-libitum diet; G2: Sham/alcohol; G3: Sham/isocaloric; G4: Ovx/ad-libitum diet; G5: Ovx/alcohol, G6: Ovx/isocaloric. Eight weeks after starting the diets, all animals were anesthetized and sacrificed. The condyles were analyzed histologically, histomorphometrically, and immunohistochemically using the antibodies for bone sialoprotein (BSP), osteocalcin (OCC) and receptor activator of nuclear factor kappa-B ligand (RANKL). Results: Histological analysis of the mandibular condyles showed that Ovx and Sham groups presented almost the same characteristics. The histomorphometric analysis showed that there was a statistically significant difference only between Ovx/isocaloric and Ovx/ad-libitum groups (p=0.049). No difference was observed in the intensity of BSP, OCC, and RANKL antibody staining between the Ovx/alcohol and the other groups. Conclusions: It may be concluded that there was no histomorphometric, histological, or RANKL, BSP, and OCC staining differences between the Ovx/alcohol group and other experimental groups.

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Section: Discussionmentioning

confidence: 62%

“…Studies have shown that ovariectomy induces bone loss in the whole mandible, including its condyle [7][8][9][10][11] . Structural defects in mandibular condyles were found in patients diagnosed with severe osteoporosis after menopause, indicating that osteoporosis can change mandibular condyle structure 12 .…”

Section: Introductionmentioning

confidence: 99%

Effects of estrogen deficiency combined with chronic alcohol consumption on rat mandibular condyle

Salgado¹,

Marchini²,

Tera³

et al. 2015

Braz. J. Oral Sci.

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“…Furthermore, estrogen negatively regulates condylar cartilage differentiation in vivo. In 4-month-old mice, ovariectomy has been shown to cause an increase in collagen type X expression after 1-8 weeks (18) . The ability of estrogen to decrease growth and differentiation of the mandibular condylar cartilage is consistent with the literature on growth plate cartilages; however, estrogen is believed to have a chondro-protective effect on articular cartilages.…”

Section: Introductionmentioning

confidence: 99%

Increased mandibular condylar growth in mice with estrogen receptor beta deficiency

Kamiya

Chen

et al. 2012

Journal of Bone and Mineral Research

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Temporomandibular joint (TMJ) disorders predominantly afflict women of childbearing age, suggesting a role for female hormones in the disease process. In long bones, estrogen acting via estrogen receptor beta (ERβ) inhibits axial skeletal growth in female mice. However, the role of ERβ in the mandibular condyle is largely unknown. We hypothesize that female ERβ deficient mice will have increased mandibular condylar growth compared with wild type (WT) female mice. This study examined female 7-, 49- and 120-day-old WT and ERβ knockout (KO) mice. There was a significant increase in mandibular condylar cartilage thickness, due to an increased number of cells, in the 49- and 120-day-old female ERβ KO compared with WT controls. Analysis in 49-day-old female ERβ KO mice revealed a significant increase in collagen type X, Pthrp and osteoprotegrin gene expression and a significant decrease in Rankl and Ihh gene expression, compared with WT controls. Subchondral bone analysis revealed a significant increase in total condylar volume and a decrease in the number of osteoclasts in the 49-day-old ERβ KO compared with WT female mice. There was no difference in cell proliferation in condylar cartilage between the genotypes. However, there were differences in the expression of proteins that regulate the cell cycle; we found a decrease in the expression of Tieg1 and p57 in the mandibular condylar cartilage from ERβ KO mice compared with WT mice. Taken together, our results suggest that ERβ deficiency increases condylar growth in female mice by inhibiting the fibrocartilage turnover.

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“…The intensity of folding and the number of immunoreactive cells to iNOS, NF-κB, and PCNA in the synovial membranes is summarized in Table 1 and by utilizing the semi-quantitative scoring method (Kishimoto et al, 2007; Min et al, 2007). …”

Section: Resultsmentioning

confidence: 99%

Effects of hyaluronan on carrageenan-induced synovitis in rat TMJ

et al. 2010

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Nitric oxide is one of many proinflammatory mediators that are involved in temporomandibular joint (TMJ) inflammatory disorder and is synthesized by inducible nitric oxide synthase (iNOS). iNOS is transcriptionally regulated by nuclear factor-κB (NF-κB) in cases of inflammation, proliferation, and apoptosis. It has also been reported that nitric oxide is positively regulated by carrageenan and negatively regulated by hyaluronan in the knee joint. The aim of this study was to histologically evaluate how inflammation and cell proliferation of the synovial membrane are affected by the exogenous administration of carrageenan and hyaluronan in the rat TMJ by investigating iNOS, NF-κB, and anti proliferating cell nuclear antigen (PCNA) immunoreactivity. As results, immunoreactive cells to iNOS, NF-κB, and PCNA were normally localized only in the synovial membrane of wild type TMJs. The numbers of immunoreactive cells were extensively larger in the carrageenan-injected synovial membranes exhibiting excessive folding, and smaller in the hyaluronan-injected synovial membranes showing a few folds. These results indicate that a carrageenan injection induced inflammation and cell proliferation especially in the synovial membrane and that hyaluronan relieved the inflammation by decreasing inflammatory molecules in the synovial membrane.

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Alteration of BMP‐4 and Runx2 expression patterns in mouse temporomandibular joint after ovariectomy

Cited by 15 publications

References 31 publications

Effects of estrogen deficiency combined with chronic alcohol consumption on rat mandibular condyle

Effects of estrogen deficiency combined with chronic alcohol consumption on rat mandibular condyle

Increased mandibular condylar growth in mice with estrogen receptor beta deficiency

Effects of hyaluronan on carrageenan-induced synovitis in rat TMJ

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