Alteration of circular RNA expression in rat dental follicle cells during osteogenic differentiation

Du, Yuguang; Li, Jing; Hou, Yuluan; Chen, Chanchan; Long, Weilin; Jiang, Hongwei

doi:10.1002/jcb.28603

Cited by 21 publications

(20 citation statements)

References 32 publications

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“…Many studies have confirmed that circRNAs played crucial roles in cells differentiation. Du et al demonstrated that circFgfr2 was a potential new target for bone formation, which promoted osteoblasts differentiation through regulating miR-133/BMP6 (Du et al, 2019). Li et al found that circular RNA CDR1 could regulate osteoblastic differentiation via the miR-7/GDF5/SMAD and p38 MAPK signaling pathway (Li et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

CircHmbox1 Targeting miRNA-1247-5p Is Involved in the Regulation of Bone Metabolism by TNF-α in Postmenopausal Osteoporosis

Liu

Huang

et al. 2020

Front. Cell Dev. Biol.

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Tumor necrosis factor-alpha (TNF-α) promotes osteoclasts differentiation to enhance bone resorption and inhibits osteoblasts differentiation to impair bone formation, which plays a central role in the development of postmenopausal osteoporosis (PMOP). Recent studies implicated an important role of circular RNAs (circRNAs) in osteoporosis. The purpose of this study is to investigate whether circRNAs might be implicated in TNF-α-regulated osteoclasts differentiation and osteoblasts differentiation in PMOP. QRT-PCR was applied to detect expression of circRNA-circHmbox1 and miR-1247-5p in TNF-α-induced osteoclasts differentiation. Western blot, TRAP staining, alkaline phosphatase staining, alizarin red S staining, transwell and cell transfection were conducted to confirm that TNF-α inhibited osteoblasts differentiation by exosomal with low circHmbox1 expression from osteoclasts. Bioinformatics analysis and luciferase reporter revealed the mechanisms of the circHmbox1/miR-1247-5p/B cell lymphoma 6 (Bcl6) interaction. In this study, we found that the level of circRNA-circHmbox1 was obviously reduced in TNF-α-induced osteoclast formation in vivo and in vitro. CircHmbox1 could inhibit RANKL-induced osteoclasts differentiation primarily through binding to microRNA-1247-5p. TNF-α decreased osteoblasts differentiation by exosomal with low circHmbox1 expression from osteoclasts. Mechanistic studies showed that microRNA-1247-5p regulated osteoclasts differentiation and osteoblasts differentiation by targeting Bcl6, which was confirmed to play opposite roles in osteoblasts differentiation and osteoclasts differentiation. Our results provide evidence that circHmbox1-targeting miR-1247-5p is involved in the regulation of bone metabolisms by TNF-α in PMOP.

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Section: Discussionmentioning

confidence: 99%

CircHmbox1 Targeting miRNA-1247-5p Is Involved in the Regulation of Bone Metabolism by TNF-α in Postmenopausal Osteoporosis

Liu

Huang

et al. 2020

Front. Cell Dev. Biol.

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“…These studies have focussed on the role of circRNA in controlling cell differentiation, which is important in the context of periodontal regeneration and tissue engineering. Herein, we undertook a scoping review of recent research into the role of circRNA in cell differentiation in vitro and periodontal tissue healing and regeneration in vivo, drawing information from 23 studies [ 37 , 38 , 39 , 40 , 41 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 ]. Much of the existing research has focused on osteogenic differentiation of periodontal stem cells, and how circRNAs might function as a sponge for miRNAs.…”

Section: The Emerging Role Of Circrnas In Periodontal Regenerationmentioning

confidence: 99%

“…The MC3T3-E1 mouse osteoblastic cell line is commonly used to study bone formation in vitro. A total of four studies investigated the role of circRNAs in this cell line [ 66 , 67 , 68 , 69 ], rat dental follicle cells (rDFCs) [ 70 ], and gingival fibroblasts cell line [ 71 ], as shown in Table 4 .…”

Section: The Emerging Role Of Circrnas In Periodontal Regenerationmentioning

confidence: 99%

“…Du et al [ 70 ] demonstrated that a total of 138 upregulated and 128 downregulated circRNAs were present in rat dental follicle cells (rDFCs, passage 3–4) after the cells had undergone 28 days of osteogenic differentiation. RT-qPCR data showed upregulated circFgfr2 (circ-fibroblast growth factor receptor 2) and downregulated circ-Tgfbr2 (circ-transforming growth factor-beta receptor 2) at 1, 2, 3, and 4 weeks after osteogenic induction.…”

Section: The Emerging Role Of Circrnas In Periodontal Regenerationmentioning

confidence: 99%

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The Emerging Regulatory Role of Circular RNAs in Periodontal Tissues and Cells

Jiao

Walsh

Ivanovski

et al. 2021

IJMS

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Periodontitis is a chronic complex inflammatory disease associated with a destructive host immune response to microbial dysbiosis, leading to irreversible loss of tooth-supporting tissues. Regeneration of functional periodontal soft (periodontal ligament and gingiva) and hard tissue components (cementum and alveolar bone) to replace lost tissues is the ultimate goal of periodontal treatment, but clinically predictable treatments are lacking. Similarly, the identification of biomarkers that can be used to accurately diagnose periodontitis activity is lacking. A relatively novel category of molecules found in oral tissue, circular RNAs (circRNAs) are single-stranded endogenous, long, non-coding RNA molecules, with covalently circular-closed structures without a 5’ cap and a 3’ tail via non-classic backsplicing. Emerging research indicates that circRNAs are tissue and disease-specific expressed and have crucial regulatory functions in various diseases. CircRNAs can function as microRNA or RNA binding sites or can regulate mRNA. In this review, we explore the biogenesis and function of circRNAs in the context of the emerging role of circRNAs in periodontitis pathogenesis and the differentiation of periodontal cells. CircMAP3K11, circCDK8, circCDR1as, circ_0062491, and circ_0095812 are associated with pathological periodontitis tissues. Furthermore, circRNAs are expressed in periodontal cells in a cell-specific manner. They can function as microRNA sponges and can form circRNA–miRNA–mRNA networks during osteogenic differentiation for periodontal-tissue (or dental pulp)-derived progenitor cells.

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“…Secreted BMP6 from other cells in DF also promoted the osteogenesis via a paracrine pathway. Interestingly, BMP6 was also considered as one of the downstream targets of circular RNAs (circRNAs) in DFC differentiation, it was upregulated in circFgfr2-enhanced DFC osteogenesis [75].…”

Section: Multipotent Differentiation Of Dfcsmentioning

confidence: 99%

Dental Follicle Cells: Roles in Development and Beyond

Zhou

Pan

et al. 2019

Stem Cells International

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Dental follicle cells (DFCs) are a group of mesenchymal progenitor cells surrounding the tooth germ, responsible for cementum, periodontal ligament, and alveolar bone formation in tooth development. Cascades of signaling pathways and transcriptional factors in DFCs are involved in directing tooth eruption and tooth root morphogenesis. Substantial researches have been made to decipher multiple aspects of DFCs, including multilineage differentiation, senescence, and immunomodulatory ability. DFCs were proved to be multipotent progenitors with decent amplification, immunosuppressed and acquisition ability. They are able to differentiate into osteoblasts/cementoblasts, adipocytes, neuron-like cells, and so forth. The excellent properties of DFCs facilitated clinical application, as exemplified by bone tissue engineering, tooth root regeneration, and periodontium regeneration. Except for the oral and maxillofacial regeneration, DFCs were also expected to be applied in other tissues such as spinal cord defects (SCD), cardiomyocyte destruction. This article reviewed roles of DFCs in tooth development, their properties, and clinical application potentials, thus providing a novel guidance for tissue engineering.

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Alteration of circular RNA expression in rat dental follicle cells during osteogenic differentiation

Cited by 21 publications

References 32 publications

CircHmbox1 Targeting miRNA-1247-5p Is Involved in the Regulation of Bone Metabolism by TNF-α in Postmenopausal Osteoporosis

CircHmbox1 Targeting miRNA-1247-5p Is Involved in the Regulation of Bone Metabolism by TNF-α in Postmenopausal Osteoporosis

The Emerging Regulatory Role of Circular RNAs in Periodontal Tissues and Cells

Dental Follicle Cells: Roles in Development and Beyond

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