Alteration of LncRNA expression in mice placentae after frozen embryo transfer is associated with increased fetal weight

Zhu, Yinian; Cai, Yunni; Zheng, Bo; Ding, Min; Zhang, Yuting; Zhou, Jianjun

doi:10.1016/j.repbio.2022.100646

Cited by 3 publications

(4 citation statements)

References 27 publications

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“…Zhu, Yuan et al. found fetal weight and placental efficiency were increased after frozen embryo transfer Field 28 . In contrast, Weinerman et al.…”

Section: Discussionmentioning

confidence: 97%

“…18,53,66 Zhu, Yuan et al found fetal weight and placental efficiency were increased after frozen embryo transfer Field. 28 In contrast, Weinerman et al found smaller fetuses arising from vitrified/warmed mouse embryos in either a natural or superovulated environment, though the differences were insignificant. 66 Our study also showed that fetal weight at E18.5 following FET was significantly lower than that following fresh embryo transfer.…”

Section: Of 18mentioning

confidence: 94%

“…Blastocyst vitrification could up‐regulate the expression of Gjb5 , an essential molecule in trophoblast differentiation in the placenta. This, in turn, can lead to an increase in the proportion of the labyrinth area associated with the exchange of nutrients and waste between maternal and fetal circulations, ultimately resulting in increased fetal weight 28 . Moreover, FET is an independent risk factor of early spontaneous miscarriage in ART cycles, 29 and the infants born are associated with a higher risk of infectious diseases and respiratory and neurologic abnormalities than those conceived after fresh embryo transfer 30 …”

Section: Introductionmentioning

confidence: 99%

“…This, in turn, can lead to an increase in the proportion of the labyrinth area associated with the exchange of nutrients and waste between maternal and fetal circulations, ultimately resulting in increased fetal weight. 28 Moreover, FET is an independent risk factor of early spontaneous miscarriage in ART cycles, 29 and the infants born are associated with a higher risk of infectious diseases and respiratory and neurologic abnormalities than those conceived after fresh embryo transfer. 30 Exposing embryos to suboptimal non-physiological environments may exert adverse impacts on the long-term metabolic health of the derived offspring.…”

Section: Introductionmentioning

confidence: 99%

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The adverse effects of vitrification on mouse embryo development and metabolic phenotype in offspring

Chen,

Zhou,

Wang

et al. 2023

The FASEB Journal

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Embryo vitrification is a standard procedure in assisted reproductive technology. Previous studies have shown that frozen embryo transfer is associated with an elevated risk of adverse maternal and neonatal outcomes. This study aimed to explore the effects of mouse blastocyst vitrification on the phenotype of vitrified‐warmed blastocysts, their intrauterine and postnatal development, and the long‐term metabolic health of the derived offspring. The vitrified‐warmed blastocysts (IVF + VT group) exhibited reduced mitochondrial activity, increased apoptotic levels, and decreased cell numbers when compared to the fresh blastocysts (IVF group). Implantation rates, live pup rates, and crown‐rump length at E18.5 were not different between the two groups. However, there was a significant decrease in fetal weight and fetal/placental weight ratio in the IVF + VT group. Furthermore, the offspring of the IVF + VT group at an age of 36 weeks had reduced whole energy consumption, impaired glucose and lipid metabolism when compared with the IVF group. Notably, RNA‐seq results unveiled disturbed hepatic gene expression in the offspring from vitrified‐warmed blastocysts. This study revealed the short‐term negative impacts of vitrification on embryo and fetal development and the long‐term influence on glucose and lipid metabolism that persist from the prenatal stage into adulthood in mice.

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“…Zhu, Yuan et al. found fetal weight and placental efficiency were increased after frozen embryo transfer Field 28 . In contrast, Weinerman et al.…”

Section: Discussionmentioning

confidence: 97%

Section: Of 18mentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The adverse effects of vitrification on mouse embryo development and metabolic phenotype in offspring

Chen,

Zhou,

Wang

et al. 2023

The FASEB Journal

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Increased Risk of Preeclampsia with Assisted Reproductive Technologies

2023

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Safety of embryo cryopreservation: insights from mid-term placental transcriptional changes

Luo,

Zhang,

et al. 2024

Reprod Biol Endocrinol

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Background In recent years, with benefits from the continuous improvement of clinical technology and the advantage of fertility preservation, the application of embryo cryopreservation has been growing rapidly worldwide. However, amidst this growth, concerns about its safety persist. Numerous studies have highlighted the elevated risk of perinatal complications linked to frozen embryo transfer (FET), such as large for gestational age (LGA) and hypertensive disorders during pregnancy. Thus, it is imperative to explore the potential risk of embryo cryopreservation and its related mechanisms. Methods Given the strict ethical constraints on clinical samples, we employed mouse models in this study. Three experimental groups were established: the naturally conceived (NC) group, the fresh embryo transfer (Fresh-ET) group, and the FET group. Blastocyst formation rates and implantation rates were calculated post-embryo cryopreservation. The impact of FET on fetal growth was evaluated upon fetal and placental weight. Placental RNA-seq was conducted, encompassing comprehensive analyses of various comparisons (Fresh-ET vs. NC, FET vs. NC, and FET vs. Fresh-ET). Results Reduced rates of blastocyst formation and implantation were observed post-embryo cryopreservation. Fresh-ET resulted in a significant decrease in fetal weight compared to NC group, whereas FET reversed this decline. RNA-seq analysis indicated that the majority of the expression changes in FET were inherited from Fresh-ET, and alterations solely attributed to embryo cryopreservation were moderate. Unexpectedly, certain genes that showed alterations in Fresh-ET tended to be restored in FET. Further analysis suggested that this regression may underlie the improvement of fetal growth restriction in FET. The expression of imprinted genes was disrupted in both FET and Fresh-ET groups. Conclusion Based on our experimental data on mouse models, the impact of embryo cryopreservation is less pronounced than other in vitro manipulations in Fresh-ET. However, the impairment of the embryonic developmental potential and the gene alterations in placenta still suggested it to be a risky operation.

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Alteration of LncRNA expression in mice placentae after frozen embryo transfer is associated with increased fetal weight

Cited by 3 publications

References 27 publications

The adverse effects of vitrification on mouse embryo development and metabolic phenotype in offspring

The adverse effects of vitrification on mouse embryo development and metabolic phenotype in offspring

Increased Risk of Preeclampsia with Assisted Reproductive Technologies

Safety of embryo cryopreservation: insights from mid-term placental transcriptional changes

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