Alterations in antioxidant enzyme activities and proline content in pea leaves under long-term drought stress

Abstract

The effects of long-term drought stress on chlorophyll, proline, protein and hydrogen peroxide (H2O2) contents, malondialdehyde (MDA) in terms of lipid peroxidation and on the changes in the activities of antioxidant enzymes such as superoxide dismutase (SOD, EC 1.15.1.1), ascorbate peroxidase (APX, EC 1.11.1.11), catalase (CAT, EC 1.11.1.6) and peroxidase (POX; EC 1.11.1.7) in the leaves of pea (Pisum sativum L.) were studied in field conditions. Chlorophyll and protein contents in leaves decreased significantly with increased drought stress. The proline content increased markedly under water deficit. MDA amounts were elevated as a result of water shortage, whereas H(2)O(2) content changed slightly in pea leaves exposed to drought stress. Drought stress markedly enhanced the activities of SOD, CAT and POX but slightly changed the activity of APX. We conclude that in field conditions, long-term water shortage increased the susceptibility to drought in peas.

Effects of individual and combined heat and drought stress during seed filling on the oxidative metabolism and yield of chickpea (Cicer arietinum) genotypes differing in heat and drought tolerance

“…birdsfoot trefoil (Lotus japonicus), Sainz et al 2010;cotton, Sekmen et al 2014). Likewise, the increased H 2 O 2 levels observed in the present study are similar to observations in drought-stressed maize (Voothuluru and Sharp 2013), sugarcane (Boaretto et al 2014) and field pea (Karataş et al 2014), and heat-stressed wheat (Kumar et al 2013a) and mungbean (Saleh et al 2007;Mansoor and Naqvi 2013). The oxidative damage was much higher in the presence of both stresses in our studies, which is similar to observations recorded in birdsfoot trefoil (Sainz et al 2010), cotton (Sekmen et al 2014) and purslane (Portulaca oleracea L., Jin et al 2015).…”

Label‐free quantitative proteomic analysis of tolerance to drought in Pisum sativum

“…Development of pea cultivars well adapted to dry conditions has been one of the major tasks in breeding programs. Thus, contrasting responses to drought have been observed and studied across the years on different pea genotypes . However, a better understanding of molecular mechanisms for drought tolerance is a priority to cope with the increasing food requirements, helping to further define drought tolerant targets in germplasm screenings.…”

Prolina livre e flavonoides totais de Lippia origanoides submetidos a níveis de saturação por base e estresse hídrico

“…Dessa forma, os resultados apresentados mostram que a produção de um determinado metabólito em resposta ao estresse hídrico é um fenômeno espécie-específica em que a aplicação de Si, pode melhorar a produção dos metabólitos de interesse e preservar o DNA para algumas espécies ou prejudicar a integridade de outras (Costa et al, 2016). É importante salientar que a função do Si no vegetal depende de fatores abióticos, bióticos e genéticos (Karatas et al, 2014;Dianat et al, 2016). Pode-se então atribuir as variações observadas dos teores de Si ao fator genético (Giongo & Bohnen 2011).…”

“…O estresse no vegetal pode ser influenciado por vários fatores abióticos, dentre eles destaca-se a limitação hídrica. A resposta fisiológica da planta ao estresse depende da intensidade e dos fatores genéticos (Karatas et al, 2014). Em situação de estresse moderado, pode induzir ajuste estomático, resultando na manutenção da turgescência celular (Monteiro et al, 2014).…”