Alterations in mitochondria membrane potential and oxidative stress in infertile men: a prospective observational study

Wang, Xia; Sharma, Rakesh K.; Gupta, Akshay; George, Valal K.; Thomas, Anthony J.; Falcone, Tommaso; Agarwal, Ashok

doi:10.1016/s0015-0282(03)00983-x

Cited by 265 publications

(191 citation statements)

References 34 publications

Supporting

Mentioning

166

Contrasting

Unclassified

Order By: Relevance

“…Oxidative stress has been suggested as one of the major cellular mechanisms involved in male infertility [105]. Oxidative stress affects the sperm membrane through lipid peroxidation [106], leading to loss of membrane stability and selective permeability, which, in turn, can cause mitochondrial dysfunctions [81], defective acrosome reaction [107], and sperm DNA fragmentation [108]. Hence, our results clearly show that viable sperm from an ejaculate of lower quality possesses protective mechanisms against the effects of the surrounding oxidative stress.…”

Section: Enriched Functions In the Low Sperm Dna Fragmentation Groupmentioning

confidence: 60%

“…Therefore, our results indicate that although viable sperm from the High sperm DNA fragmentation group can obtain energy through anaerobic glycolysis and fatty acid beta oxidation, the oxidative phosphorylation and triacylglycerol turnover are defective. This can be a consequence of oxidative stress occurring in the ejaculate from this group, which can alter sperm mitochondrial activity [81]. Because mitochondria are usually the first site for alterations triggered by oxidative stress [81], this can be occurring in the High group even in the presence of some proteins involved with sperm protection, such as ODF1 and prostasomal proteins.…”

Section: Enriched Functions In the Low Sperm Dna Fragmentation Groupmentioning

confidence: 99%

“…This can be a consequence of oxidative stress occurring in the ejaculate from this group, which can alter sperm mitochondrial activity [81]. Because mitochondria are usually the first site for alterations triggered by oxidative stress [81], this can be occurring in the High group even in the presence of some proteins involved with sperm protection, such as ODF1 and prostasomal proteins. These impaired processes might affect sperm function, especially regarding capacitation, leading to the related negative impact on human reproduction.…”

Section: Enriched Functions In the Low Sperm Dna Fragmentation Groupmentioning

confidence: 99%

See 2 more Smart Citations

Unraveling the sperm proteome and post-genomic pathways associated with sperm nuclear DNA fragmentation

Intasqui

Camargo

Giudice

et al. 2013

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose Sperm DNA fragmentation has been suggested as a marker for infertility diagnosis and prognosis. Hence, understanding its impact on male physiology and post-genomic pathways would be clinically important. We performed the proteomics and functional enrichment analyses of viable spermatozoa from ejaculates with low and high sperm DNA fragmentation to identify protein expression and pathways altered in association with sperm DNA fragmentation. Methods Sperm DNA fragmentation using the Comet assay and the Komet 6.0.1 software was assessed in raw samples from 89 subjects from a human reproduction service. The Low and High sperm DNA fragmentation groups were formed according to the Olive Tail Moment variable. Spermatozoa proteins from these groups were pooled and analyzed by a shotgun proteomic approach (2D nanoUPLC-ESI-MS E ). Differentially expressed proteins were used for a functional enrichment study. Results Two hundred and fifty-seven proteins were identified or quantified in sperm from the Low and High sperm DNA fragmentation groups. Of these, seventy-one proteins were exclusively or overexpressed in the Low group, whereas twenty-three proteins were exclusively or overexpressed in the High group. One hundred and sixty-three proteins were conserved between these groups. We also functionally related the differentially expressed proteins in viable spermatozoa from the groups. Processes such as triacylglycerol metabolism, energy production, protein folding, response to unfolded proteins, and cellular detoxification were found to be altered in these cells. Conclusions Sperm DNA fragmentation is associated with differential protein expression in viable spermatozoa. These proteins may potentially be used as biomarkers for sperm DNA integrity.

show abstract

Section: Enriched Functions In the Low Sperm Dna Fragmentation Groupmentioning

confidence: 60%

Section: Enriched Functions In the Low Sperm Dna Fragmentation Groupmentioning

confidence: 99%

Section: Enriched Functions In the Low Sperm Dna Fragmentation Groupmentioning

confidence: 99%

See 1 more Smart Citation

Unraveling the sperm proteome and post-genomic pathways associated with sperm nuclear DNA fragmentation

Intasqui

Camargo

Giudice

et al. 2013

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

“…Imbalances between ROS production and semen antioxidant capacity will lead to oxidative stress [9,10], which will ultimately result in oxidation of membrane polyunsaturated fatty acids [14,15], loss of mitochondrial membrane potential [16], and single and double strand DNA fragmentation [17].…”

Section: Discussionmentioning

confidence: 99%

“…Sperm are especially susceptible to OS because they present high plasma membrane concentrations of polyunsaturated fatty acids (PUFA)-methylene groups present between double bonds in the carbon chain are sites where hydrogen molecules may easily be removed by ROS [14,15]-and lipid peroxidation will eventually lead to loss of mitochondrial activity [16] and increased DNA fragmentation [17].…”

Section: Introductionmentioning

confidence: 99%

Effect of leukocytospermia and processing by discontinuous density gradient on sperm nuclear DNA fragmentation and mitochondrial activity

Fariello

Giudice

Spaine

et al. 2009

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose To assess the effect of leukocytospermia and semen processing on sperm DNA and mitochondria. Methods Twenty-two patients with and 41 without leukocytospermia were included. Sperm DNA fragmentation was assessed by the Comet assay, and mitochondrial activity by a colorimetric method for active mitochondria. Semen was processed using Percoll, and motility, DNA fragmentation, and mitochondrial activity were analyzed pre-and post-processing. Results No differences were observed in age, abstinence, volume, sperm morphology, progressive motility, concentration, and vitality (p>0.10). Variables were grouped according to time (pre-vs post-processing) and group (leukocytospermia vs non-leukocytospermia) because no interactions could be observed. Leukocytospermia was associated to increased DNA fragmentation, while semen processing led to a decrease in DNA fragmentation and to increased mitochondrial activity. Conclusion While semen processing selects sperm with higher rates of DNA integrity independent of the presence or absence of leukocytes in semen, samples without leukocytospermia present more sperm without DNA fragmentation. Semen processing also selects sperm with higher mitochondrial activity.

show abstract

Assessment of Prior Infection With Hepatitis B Virus and Fecundability in Couples Planning Pregnancy

Zhao,

Xuan,

Zhang

et al. 2023

JAMA Netw Open

View full text Add to dashboard Cite

IMPORTANCEThe association of hepatitis B virus (HBV) infection with reduced fecundability among reproductive-aged couples lacks large-population, in-depth study evidence.OBJECTIVETo investigate the association of HBV infection with time to pregnancy in couples planning pregnancy, and to explore whether this association varied by gravidity, health statuses, or lifestyles.DESIGN, SETTING, AND PARTICIPANTSThis is a population-based cohort study of Chinese couples participating in the National Free Preconception Check-up Projects during 2015 to 2017. They were planning pregnancy and were followed-up every 3 months until getting pregnant, as confirmed by gynecologic ultrasonography, or were followed-up for 1 year. Data were analyzed between March 1, 2022, and September 30, 2022.MAIN OUTCOMES AND MEASURESThe main outcome was time to pregnancy, assessed using fecundability hazard ratios (HRs). The Cox proportional hazards regression models were used to estimate the association of HBV infection with fecundability.RESULTSAmong 2 419 848 couples (mean [SD] age, 27.87 [5.20] years for women and 29.58 [5.50] years for men), 126 728 women (5.24%) and 156 572 men (6.47%) were infected with HBV. Compared with the HBV-negative group, the fecundability of both women and men in the HBV-positive group decreased by 5% (HR, 0.95; 95% CI, 0.94-0.95). Compared with couples in which both partners were HBV negative, the fecundability of those in which both partners were HBV positive declined by 6% (HR, 0.94; 95% CI, 0.93-0.96) among all couples, by 3% (HR, 0.97; 95% CI, 0.95-0.99) among nulligravidas couples, and by 7% (HR, 0.93; 95% CI, 0.91-0.95) among multigravidas couples. Both the female-male and couple models suggested that the association of HBV infection with decreased fecundability was more pronounced in couples with multigravidas. The negative association was greater in people with overweight and obesity and was inconsistent in certain subgroups; in particular, it was more pronounced in women with reproductive tract infections, normal fasting plasma glucose, and no alcohol intake and in men with normal blood pressure.CONCLUSIONS AND RELEVANCEIn this population-based cohort study, HBV infection was associated with decreased fecundability in a general reproductive-aged population, especially in couples with multigravidas. For women and men with certain health statuses and lifestyles, a comprehensive consideration of this association is recommended to provide personalized fertility guidance.

show abstract

Alterations in mitochondria membrane potential and oxidative stress in infertile men: a prospective observational study

Cited by 265 publications

References 34 publications

Unraveling the sperm proteome and post-genomic pathways associated with sperm nuclear DNA fragmentation

Unraveling the sperm proteome and post-genomic pathways associated with sperm nuclear DNA fragmentation

Effect of leukocytospermia and processing by discontinuous density gradient on sperm nuclear DNA fragmentation and mitochondrial activity

Assessment of Prior Infection With Hepatitis B Virus and Fecundability in Couples Planning Pregnancy

Contact Info

Product

Resources

About