Alterations in the composition of membrane glycero-and sphingolipids in the course of Flammulina velutipes surface culture development

Котлова, Е. Р.; Senik, Svetlana V.; Kücher, T.; Шаварда, А. Л.; Kiyashko, A. A.; Psurtseva, Nadezhda V.; Sinyutina, N. F.; Zubarev, Roman A.

doi:10.1134/s002626170902009x

Cited by 12 publications

(9 citation statements)

References 25 publications

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“…1). Similar results were observed in previous studies of surface cultures of F. velutipes and Lentinula edodes grown on complete organic media (Sakai and Kajiwara, 2004;Kotlova et al, 2009). In both studies the PC/PE ratio in young and actively growing mycelium was higher than at later stages of development.…”

Section: Expression Of Cho2 and Cpt1 Genessupporting

confidence: 91%

“…After harvesting the mycelium, lipid extracts were prepared with isopropanol according to the Nichols method (Nichols, 1963) with modifications as previously described (Kotlova et al, 2009). Concentrated lipid preparations were separated on TLC silica gel 60 10 Â 10 cm plates (Merck, Germany) in two dimensions in a solvent system of chloroform-methanol-water (65:25:4) in the first direction and chloroform-acetone-methanol-acetic acid-water (50:20:10:10:5) in the second direction (Benning et al, 1995).…”

Section: Lipid Extraction and Quantitative Lipid Analysismentioning

confidence: 99%

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Diacylglyceryltrimethylhomoserine content and gene expression changes triggered by phosphate deprivation in the mycelium of the basidiomycete Flammulina velutipes

Senik¹,

Maloshenok²,

Котлова³

et al. 2015

Phytochemistry

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Section: Expression Of Cho2 and Cpt1 Genessupporting

confidence: 91%

Section: Lipid Extraction and Quantitative Lipid Analysismentioning

confidence: 99%

Diacylglyceryltrimethylhomoserine content and gene expression changes triggered by phosphate deprivation in the mycelium of the basidiomycete Flammulina velutipes

Senik¹,

Maloshenok²,

Котлова³

et al. 2015

Phytochemistry

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“…At the same time, recent studies have shown that there is a relationship between fungal growth and development and lipid metabolism. It is known that lipid rafts can participate in the polar growth of hyphal apices [ 13 ]; the ratio of major membrane lipids, particularly phosphatidylcholines (PC) and phosphatidylethanolamines (PE), correlates with the age of the fungal colony; the increase in phospholipids esterified by C18:2 Δ9,12 and C18:3 Δ9,12,15 fatty acids accompanies the fruit body development [ 14 ]. In Aspergillus nidulans, PC affects hyphal extension, membrane integrity, and chitin synthesis [ 15 , 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Heterogeneous Distribution of Phospholipid Molecular Species in the Surface Culture of Flammulina velutipes: New Facts about Lipids Containing α-Linolenic Fatty Acid

Senik

Manzhieva

Maloshenok

et al. 2023

JoF

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Mycelial fungi grow as colonies consisting of polar growing hyphae, developing radially from spore or inoculum. Over time, the colony develops, hyphae are subject to various exogenous or endogenous stimuli, and mycelium becomes heterogeneous in growth, gene expression, biosynthesis, and secretion of proteins and metabolites. Although the biochemical and molecular mechanisms of mycelium heterogeneity have been the subject of many studies, the role of lipids in colony development and zonality is still not understood. This work was undertaken to extend our knowledge of mycelium heterogeneity and to answer the question of how different lipid molecular species are distributed in the surface colony of the basidial fungus Flammulina velutipes and how this distribution correlates with its morphology. The heterogeneity in the lipid metabolism and lipid composition of the fungal mycelium was demonstrated. According to the real-time PCR and LC-MS/MS results, the expression of genes of PC metabolism, accumulation of phospholipid classes, and degree of unsaturation of PC and PE increased in the direction from the center to the periphery of the colony. The peripheral zone of the colony was characterized by a higher value of the PC/PE ratio and a higher level of phospholipids esterified by linolenic acid. Considering that the synthesis of phospholipids in fungi occurs in different ways, we also conducted experiments with deuterium-labeled phospholipid precursors and found out that the Kennedy pathway is the predominant route for PC biosynthesis in F. velutipes. The zonal differences in gene expression and lipid composition can be explained by the participation of membrane lipids in polar growth maintenance and regulation.

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“…Lipids were extracted with a mixture of isopropanol and chloroform (1 : 1), according to Nichols with modifications . Individual phospholipids and sphingolipids were analyzed with 2D TLC, according to Vaskovsky and Terekhova with modifications.…”

Section: Methodsmentioning

confidence: 99%

Sterol binding by methyl‐β‐cyclodextrin and nystatin – comparative analysis of biochemical and physiological consequences for plants

et al. 2014

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The dependence of membrane function on its sterol component has been intensively studied with model lipids and isolated animal membranes, but to a much lesser extent with plant membranes. Depleting membrane sterols could be predicted to have a strong effect on membrane activity and have harmful physiological consequences. In this study, we characterized membrane lipid composition, membrane permeability for ions, some physiological parameters, such as H 2 O 2 accumulation, formation of autophagosomal vacuoles, and expression of peroxidase and autophagic genes, and cell viability in the roots of wheat (Triticum aestivum L.) seedlings in the presence of two agents that specifically bind to endogenous sterols. The polyene antibiotic nystatin binds to endogenous sterols, forming so-called 'nystatin pores' or 'channels' in the membrane, and methyl-b-cyclodextrin has the capacity to sequester sterols in its hydrophobic core. Unexpectedly, although application of both methyl-b-cyclodextrin and nystatin reduced the sterol content, their effects on membrane permeability, oxidative status and autophagosome formation in roots differed dramatically. For comparison, we also tested the effects of the antibiotic gramicidin S, which does not bind to sterols but forms nonspecific channels in the membrane. Gramicidin S considerably increased membrane permeability, caused oxidative stress, and reduced cell viability. Our results suggest that a decrease in the sterol content is, in itself, not sufficient to have deleterious effects on a cell. The disturbance of membrane integrity, rather than the decrease in the sterol content, is responsible for the toxicity of sterol-binding compounds.

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Alterations in the composition of membrane glycero-and sphingolipids in the course of Flammulina velutipes surface culture development

Cited by 12 publications

References 25 publications

Diacylglyceryltrimethylhomoserine content and gene expression changes triggered by phosphate deprivation in the mycelium of the basidiomycete Flammulina velutipes

Diacylglyceryltrimethylhomoserine content and gene expression changes triggered by phosphate deprivation in the mycelium of the basidiomycete Flammulina velutipes

Heterogeneous Distribution of Phospholipid Molecular Species in the Surface Culture of Flammulina velutipes: New Facts about Lipids Containing α-Linolenic Fatty Acid

Sterol binding by methyl‐β‐cyclodextrin and nystatin – comparative analysis of biochemical and physiological consequences for plants

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