Alterations in the main steps of reverse cholesterol transport in male patients with primary hypertriglyceridemia and low HDL-cholesterol levels

Brites, Fernando; Bonavita, Carla D; Geitere, Catherine De; Cloës, Marcelo; Delfly, Bernard; Yael, Mario J; Fruchart, Jean‐Charles; Wikinski, Regina; Castro, Graciela

doi:10.1016/s0021-9150(99)00452-9

Cited by 67 publications

(48 citation statements)

References 47 publications

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“…In adults, this association renders HDL proatherogenic, with a loss or reduction of many of its functions, including its reverse cholesterol transport and antioxidant properties (15,(28)(29)(30). Therefore, we suggest that this would also be the case in SAA-enriched HDL from overweight and obese children; in support of this concept, we have shown that the activities of HDL 2 -CETP and HDL 2 -LCAT were also influenced in these overweight and obese cohorts.…”

Section: Discussionsupporting

confidence: 62%

High-density lipoprotein subfractions display proatherogenic properties in overweight and obese children

et al. 2013

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Background:In adults, obesity-driven inflammation can lead to increased cardiovascular disease (CVD). However, information regarding childhood obesity and its inflammatory sequelae is less well defined. Serum amyloid-A (SAA) is an inflammatory molecule that rapidly associates with highdensity lipoproteins (HDLs) and renders them dysfunctional. Therefore, SAA may be a useful biomarker to identify increased CVD potential in overweight and obese children. Methods: Young Hearts 2000 is a cross-sectional cohort study in which 92 children who were obese were matched for age and sex with 92 overweight and 92 lean children. HDL 2 and HDL 3 (HDL 2&3 ) were isolated from plasma by a three-step rapid-ultracentrifugation procedure. SAA was measured in serum and HDL 2&3 by an enzyme-linked immunosorbent assay procedure, and the activities of cholesterol ester transfer protein (CETP) and lecithin cholesteryl acyltransferase (LCAT) were measured by fluorimetric assays. results: Trends across the groups indicated that SAA increased in serum and HDL 2&3 as BMI increased, as did HDL 2 -CETP and HDL 2 -LCAT activities. conclusion: These results have provided evidence that overweight and obese children are exposed to an inflammatory milieu that impacts the antiatherogenic properties of HDL and that could increase CVD risk. This supports the concept that it is important to target childhood obesity to help minimize future cardiovascular events.

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Section: Discussionsupporting

confidence: 62%

High-density lipoprotein subfractions display proatherogenic properties in overweight and obese children

et al. 2013

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“…The latter correlation is in accordance with earlier studies that reported that SR-BI-rich cells are highly sensitive to HDL-PL concentrations (38,39). The correlation between cholesterol efflux capacity from cAMP-treated J774 cells and HDL-PL was also found in patients with primary hypertriglyceridemia by Brites et al (40). Recent results of Yancey et al (28) using plasma from human apoA-I transgenic mice, however, are not entirely consistent with these data.…”

Section: Discussionmentioning

confidence: 89%

Moderate alcohol consumption increases cholesterol efflux mediated by ABCA1

Beulens

Sierksma

Tol

et al. 2004

Journal of Lipid Research

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Moderate alcohol consumption increases HDL cholesterol, which is involved in reverse cholesterol transport (RCT). The aim of this study was to investigate the effect of moderate alcohol consumption on cholesterol efflux, using J774 mouse macrophages and Fu5AH cells, and on other parameters in the RCT pathway. Twenty-three healthy men (45-65 years) participated in a randomized, partially diet-controlled, crossover trial. They consumed four glasses of whisky (40 g of alcohol) or water daily for 17 days. After 17 days of whisky consumption, serum capacity to induce ABCA1-dependent cholesterol efflux from J774 mouse macrophages was increased by 17.5% ( P ‫؍‬ 0.027) compared with water consumption. Plasma capacity to induce cholesterol efflux from Fu5AH cells increased by 4.6% ( P ‫؍‬ 0.002). Pre ␤ -HDL, apolipoprotein A-I (apoA-I), and lipoprotein A-I:A-II also increased by 31.6, 6.2, and 5.7% ( P Ͻ 0.05), respectively, after whisky consumption compared with water consumption. Changes of cAMP-stimulated cholesterol efflux correlated ( r ‫؍‬ 0.65, P Ͻ 0.05) with changes of apoA-I but not with changes of pre ␤ -HDL ( r ‫؍‬ 0.30, P ‫؍‬ 0.18). Cholesterol efflux capacities from serum of lean men were higher than those from overweight men.In conclusion, this study shows that moderate alcohol consumption increases the capacity of serum to induce cholesterol efflux from J774 mouse macrophages, which may be mediated by ABCA1.

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“…Consistent with this observation, dense HDL subfractions from HALP subjects were enriched in TG and total protein and depleted of CE and FC. The diminished capacity of TG-rich HDL particles to acquire cellular cholesterol has been recently demonstrated, 33 suggesting that they may possess diminished capacity to acquire oxidized CE and FC from LDL. The pro-oxidant role of TG enrichment in HDL subfractions is entirely consistent with positive correlations between plasma levels of 8-isoprostanes and percent TG content of small dense HDL3a and 3b subfractions.…”

Section: Discussionmentioning

confidence: 99%

Antioxidative Activity of HDL Particle Subspecies Is Impaired in Hyperalphalipoproteinemia: Relevance of Enzymatic and Physicochemical Properties

Kontush

Faria

Chantepie

et al. 2004

ATVB

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Objective-Hyperalphalipoproteinemia (HALP) is characterized by elevated plasma levels of high-density lipoprotein (HDL) particles with altered composition, metabolism, and function. The impact of such modification on antioxidative activities of HDL subfractions is indeterminate. Methods and Results-Gradient fractionation revealed that buoyant HDL2b and 2a and small dense HDL3b and 3c levels were elevated up to 2.0-fold in HALP subjects (nϭ9; mean plasma HDL cholesterol, 79 mg/dL) with low hepatic lipase activity. HDL2a, 3a, 3b, and 3c displayed lower specific antioxidative activity (sAA) during low-density lipoprotein (LDL) oxidation (Ϫ15% to Ϫ86%, on a unit particle mass basis) than their normolipidemic counterparts (nϭ13). LDL oxidation was delayed by control HDL3a, 3b, and 3c (up to Ϫ79%) but specifically by HDL3c (Ϫ54%) in HALP. Paraoxonase activity was deficient in all HALP HDL subfractions. Paraoxonase, PAF-AH, and LCAT activities together accounted for Ϸ50% of variation in sAA. Abnormal chemical composition of HDL3b and 3c (cholesterol-deficient, triglyceride-enriched) in HALP was associated with impaired sAA. Systemic oxidative stress (as plasma 8-isoprostanes) tended to be elevated (1.5-fold) in HALP and negatively correlated with sAA (as TBARS). Conclusions-Intrinsic

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Alterations in the main steps of reverse cholesterol transport in male patients with primary hypertriglyceridemia and low HDL-cholesterol levels

Cited by 67 publications

References 47 publications

High-density lipoprotein subfractions display proatherogenic properties in overweight and obese children

High-density lipoprotein subfractions display proatherogenic properties in overweight and obese children

Moderate alcohol consumption increases cholesterol efflux mediated by ABCA1

Antioxidative Activity of HDL Particle Subspecies Is Impaired in Hyperalphalipoproteinemia: Relevance of Enzymatic and Physicochemical Properties

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