Alterations in the Phase Portrait and Electrophoretic Mobility of Erythrocytes in Various Diseases

Abstract

The aim of the investigation was to study a phase portrait, electrophoretic mobility of erythrocytes (EPME), and clinical and laboratory blood indices in patients with various types of diseases. Materials and Methods. A phase portrait, EPME, and clinical and laboratory blood indices have been studied in patients with cardiovascular, bronchopulmonary, gynecological, and gastroenterological diseases. The phase portrait was investigated using laser interferometry technique, EPME was determined by microelectrophoresis. The clinical and laboratory indices included counting the number of erythrocytes and leukocytes, measurements of hemoglobin content and ESR by means of standard clinical methods. The phase portrait and EPME were studied under the action of adrenaline and cortisol in experiments in vitro. Results. Using laser interferometry, phase images of erythrocytes in cardiovascular, gastroenterological, bronchopulmonary, and gynecological diseases have been obtained. The analysis of the gallery of erythrocyte phase images has shown that alterations were unidirectional in all types of pathologies and were characterized by the increase of echinocyte number. The morphological modification of erythrocytes correlated with EPME changes and clinical and laboratory blood indices and reflected the degree of stress and triggering of adaptive processes in the course of the conducted therapy. Influence of stress-realizing systems has been confirmed by the in vitro experiments under the action of adrenaline and cortisol. Conclusion. Indices of EPME and erythrocyte phase portrait obtained with the help of the current techniques of cellular microelectrophoresis and laser interferometry provide the opportunity to obtain new information on the internal structure of cytoobjects without special preparations of specimens and contrast or fluorescent probes. These indices may be applied for quantitative characteristic of the stress reaction degree and the development of adaptive processes which is of great importance for the transition to personified medicine.

RETRACTED: Dynamics of hematological and oxidative parameters characterizing the nonspecific resistance of cows under technological stress

“…In connection with the foregoing, the aim of the work was to study the dynamics of biochemical, hormonal and oxidative parameters in the blood of cattle from the point of view of nonspecific resistance of animals under the action of technological stress [11][12][13][14][15][16][17][18][19][20].…”

The Effect of Molecular Hydrogen on Functional States of Erythrocytes in Rats with Simulated Chronic Heart Failure

“…In addition, the results of our previous studies indicate that the change in EPM allows us to characterize the development of stress reactions and the inclusion of adaptive processes of the organism. The registered primary decrease in EPM is associated with an increase in the level of circulating catecholamines in the blood and an increase in the sensitivity of adrenoreceptors to them [ 44 ]. During the deployment of the stress reaction, catecholamines, activating the release of adrenocorticotropic hormone (ACTH), stimulate an increase in the level of adrenal cortex hormones in the blood, which leads to an increase in cortisol levels and an increase in EPM, i.e., we observe the second phase of the stress reaction, which is accompanied by recovery processes and increased resistance of the body [ 45 ].…”

Effects of lipoperoxidation and mitochondrial state on milk yield of dairy cows under technological stress

“…Serum cortisol level was determined using an automatic ELISA analyzer (Evolis Twin Plus, Russia) (Asuzu et al 2023). The MDA concentration was determined by reaction with thiobarbituric acid to form a coloured trimethine complex, and the absorption of this complex was recorded with the help of spectrophotometrically at 530 nm (Deryugina et al 2019a).…”