Alterations in the Young’s modulus and volumetric properties of chondrocytes isolated from normal and osteoarthritic human cartilage

Jones, Wendy R.; Ting‐Beall, H. Ping; Lee, Greta M.; Kelley, Scott S.; Hochmuth, Robert M.; Guilak, Farshid

doi:10.1016/s0021-9290(98)00166-3

Cited by 257 publications

(217 citation statements)

References 26 publications

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“…Due to the complexity of visualising MA of substrate adhered cells, previous MA studies have focused primarily on cells suspended in media. In particular, suspended endothelial cells [2][3][4], suspended chondrocytes [5][6][7], suspended stem cells [8], and suspended fibroblasts [9] have been tested using the MA technique. However such studies are of limited value as these cell phenotypes are typically not found suspended in vivo; rather, they adhere to an extra cellular matrix.…”

Section: Discussionmentioning

confidence: 99%

“…However, primarily due to the technical complexity of performing MA on cells adhered to a substrate or extra cellular matrix (ECM), the MA technique has largely been limited to the investigation of un-adhered cells suspended in media. Previous experimental MA studies have focused on suspended endothelial cells [2][3][4], suspended chondrocytes [5][6][7], suspended stem cells [8], and suspended fibroblasts [9]. Critically, in suspended cells a fibrillous contractile actin cytoskeleton is not developed [2,10].…”

Section: Introductionmentioning

confidence: 99%

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On the role of the actin cytoskeleton and nucleus in the biomechanical response of spread cells

et al. 2014

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

On the role of the actin cytoskeleton and nucleus in the biomechanical response of spread cells

et al. 2014

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“…64 Therefore the cell properties were kept constant at the following values: Ha = 1 kPa , K = 10 −15 m 4 N −1 s −1 and ν = 0.4. 22,26 The finite element package SEPRAN was employed for the numerical implementation. 54 The transport problem Eq.…”

Section: Methodsmentioning

confidence: 99%

The Local Matrix Distribution and the Functional Development of Tissue Engineered Cartilage, a Finite Element Study

et al. 2004

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Abstract-Assessment of the functionality of tissue engineered cartilage constructs is hampered by the lack of correlation between global measurements of extra cellular matrix constituents and the global mechanical properties. Based on patterns of matrix deposition around individual cells, it has been hypothesized previously, that mechanical functionality arises when contact occurs between zones of matrix associated with individual cells. The objective of this study is to determine whether the local distribution of newly synthesized extracellular matrix components contributes to the evolution of the mechanical properties of tissue engineered cartilage constructs. A computational homogenization approach was adopted, based on the concept of a periodic representative volume element. Local transport and immobilization of newly synthesized matrix components were described. Mechanical properties were taken dependent on the local matrix concentration and subsequently the global aggregate modulus and hydraulic permeability were derived. The transport parameters were varied to assess the effect of the evolving matrix distribution during culture. The results indicate that the overall stiffness and permeability are to a large extent insensitive to differences in local matrix distribution. This emphasizes the need for caution in the visual interpretation of tissue functionality from histology and underlines the importance of complementary measurements of the matrix's intrinsic molecular organization.

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“…I Journril q f Orllwpuedic R~WLWCII 22 (2004) [131][132][133][134][135][136][137][138][139] matrices [12]. In previous studies, we have shown that the mechanical behavior of the chondrocyte is that of a viscoelastic solid [26,48], and it has been assumed that the chondrocyte cytoskeleton is responsible for this "solid-like'' elastic response due to its distribution and structure within the cell [lo]. However, the sources of viscoelasticity in the cell are not fully understood and may be attributed to flow-dependent (fluid-solid interactions and fluid viscosity) [28,46] as well as flow-independent mechanisms (viscoelasticity of the cytoskeleton) [5,8,20,43,45,47].…”

Section: Introductionmentioning

confidence: 99%

The role of the cytoskeleton in the viscoelastic properties of human articular chondrocytes

Trickey

Vail

Guilak

2004

Journal Orthopaedic Research

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202

182

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Biomechanical factors are believed to play a n important role in regulating the metabolic activity of chondrocytes in articular cartilage. Previous studies suggest that cytoskeletal proteins such as actin, vimentin, and tubulin influence cellular mechanical properties, and may therefore influence the mechanical interactions between the chondrocyte and the surrounding tissue matrix. In this study, we investigated the role of specific cytoskeletal components on the mechanical properties of individual chondrocytes isolated from normal or osteoarthritic hip articular cartilage. Chondrocytes were exposed to a range of concentrations of chemical agents that disrupt the primary cytoskeletal elements (cytochalasin D for F-actin microfilaments, acrylamide for vimentin intermediate filaments, and colchicine for microtubules). Chondrocyte mechanical properties were determined using the micropipette aspiration technique coupled with a viscoelastic solid model of the cell. Chondrocyte stiffness (elastic modulus) was significantly increased with osteoarthritis. With increasing cytochalasin D treatment, chondrocyte stiffness decreased by up to 90%) and apparent viscosity decreased by up to SOYO. The effect of cytochalasin D was greater on normal chondrocytes than those isolated from osteoarthritic cartilage. Treatment with acrylamide also decredsed the moduli and viscosity, but only at the highest concentration tested. No consistent changes in cell mechanical properties were observed with colchicine treatment. These findings suggest that microfilaments and possibly intermediate filaments provide the viscoelastic properties of the chondrocyte, and changes in the structure and properties of these cytoskeletal elements may reflect changes in the chondrocyte with osteoarthritis.

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Alterations in the Young’s modulus and volumetric properties of chondrocytes isolated from normal and osteoarthritic human cartilage

Cited by 257 publications

References 26 publications

On the role of the actin cytoskeleton and nucleus in the biomechanical response of spread cells

On the role of the actin cytoskeleton and nucleus in the biomechanical response of spread cells

The Local Matrix Distribution and the Functional Development of Tissue Engineered Cartilage, a Finite Element Study

The role of the cytoskeleton in the viscoelastic properties of human articular chondrocytes

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