Alterations in Water Status, Endogenous Abscisic Acid Content, and Expression of rab18 Gene during the Development of Freezing Tolerance in Arabidopsis thaliana

Lång, V.; Mäntylä, Einar; Welin, Björn; Sundberg, Björn; Palva, E. Tapio

doi:10.1104/pp.104.4.1341

Cited by 257 publications

(168 citation statements)

References 31 publications

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“…We investigated the possible involvement of ABA in Sus gene expression by two different approaches : (1) by feeding ABA to detached leaves and (2) by using ABAdeficient Arabidopsis mutants in a cold stress experiment. ABA had little or no effect on steady-state levels of Sus1 mRNA, whereas an ABA-responsive gene (rab18) [24] that was used as a control was strongly induced by the treatment (results not shown). This confirmed that ABA was indeed taken up efficiently by the leaves via the transpiration stream.…”

Section: Role Of Aba In Sus Expressionmentioning

confidence: 96%

“…Its up-regulation did not involve ABA signalling, as demonstrated by the feeding of ABA to detached leaves (results not shown) and the use of ABA-deficient mutants under cold treatment (Figure 4). rab18, an ABAresponsive gene [24] used in these studies as a control, was very strongly induced on feeding with ABA, whereas its expression was moderate in wild-type plants under cold stress. It has been shown [24] that low temperature results in only small changes in endogenous ABA level ; in accord with that, only relatively small changes in rab18 mRNA level were observed.…”

Section: Sus1 Is Induced By Changes In Leaf Osmotic Potential Via An mentioning

confidence: 99%

“…rab18, an ABAresponsive gene [24] used in these studies as a control, was very strongly induced on feeding with ABA, whereas its expression was moderate in wild-type plants under cold stress. It has been shown [24] that low temperature results in only small changes in endogenous ABA level ; in accord with that, only relatively small changes in rab18 mRNA level were observed. Cold exposure resulted in the accumulation of soluble sugars in the leaves and a decrease in leaf osmotic potential ( Figure 1B) ; these processes are commonly observed during low-temperature stress [1,31].…”

Section: Sus1 Is Induced By Changes In Leaf Osmotic Potential Via An mentioning

confidence: 99%

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Sugar/osmoticum levels modulate differential abscisic acid-independent expression of two stress-responsive sucrose synthase genes in Arabidopsis

Déjardin

Sokolov

Kleczkowski

1999

Biochemical Journal

114

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Sucrose synthase (Sus) is a key enzyme of sucrose metabolism. Two Sus-encoding genes (Sus1 and Sus2) from Arabidopsis thaliana were found to be profoundly and differentially regulated in leaves exposed to environmental stresses (cold stress, drought or O2 deficiency). Transcript levels of Sus1 increased on exposure to cold and drought, whereas Sus2 mRNA was induced specifically by O2 deficiency. Both cold and drought exposures induced the accumulation of soluble sugars and caused a decrease in leaf osmotic potential, whereas O2 deficiency was characterized by a nearly complete depletion in sugars. Feeding abscisic acid (ABA) to detached leaves or subjecting Arabidopsis ABA-deficient mutants to cold stress conditions had no effect on the expression profiles of Sus1 or Sus2, whereas feeding metabolizable sugars (sucrose or glucose) or non-metabolizable osmotica [poly(ethylene glycol), sorbitol or mannitol] mimicked the effects of osmotic stress on Sus1 expression in detached leaves. By using various sucrose/mannitol solutions, we demonstrated that Sus1 was up-regulated by a decrease in leaf osmotic potential rather than an increase in sucrose concentration itself. We suggest that Sus1 expression is regulated via an ABA-independent signal transduction pathway that is related to the perception of a decrease in leaf osmotic potential during stresses. In contrast, the expression of Sus2 was independent of sugar/osmoticum effects, suggesting the involvement of a signal transduction mechanism distinct from that regulating Sus1 expression. The differential stress-responsive regulation of Sus genes in leaves might represent part of a general cellular response to the allocation of carbohydrates during acclimation processes.

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Section: Role Of Aba In Sus Expressionmentioning

confidence: 96%

Section: Sus1 Is Induced By Changes In Leaf Osmotic Potential Via An mentioning

confidence: 99%

Section: Sus1 Is Induced By Changes In Leaf Osmotic Potential Via An mentioning

confidence: 99%

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Sugar/osmoticum levels modulate differential abscisic acid-independent expression of two stress-responsive sucrose synthase genes in Arabidopsis

Déjardin

Sokolov

Kleczkowski

1999

Biochemical Journal

114

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“…The expression of tomato group 2 LEA protein (Le4) in yeast partially ameliorated the detrimental effects of ionic and freezing stress by conferring tolerance to high concentration of KCl but not to NaCl or sorbitol (Zhang et al, 2000). However, overexpression studies undertaken using DHNs such as CAP85 (Kaye et al, 1998) as well as C. plantagineum DHN (Iturriaga and Bartels, 1992) and RAB18 (Lang et al,1994) had no effect on freezing or drought tolerance. Overproduction of citrus dehydrin (CoCOR19) in tobacco resulted in slight decrease in ion leakage under chilling and freezing stress.…”

Section: Structure and Functional Studies Of Dhnamentioning

confidence: 99%

Genetic approaches towards overcoming water deficit in plants - special emphasis on LEAs

Khurana

Vishnudasan

Chhibbar

2008

Physiol Mol Biol Plants

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Water deficit arises as a result of low temperature, salinity and dehydration, thereby affecting plant growth adversely and making it imperative for plants to surmount such situations by acclimatizing/adapting at various levels. Water deficit stress results in significant changes in gene expression, mediated by interconnected signal transduction pathways that may be triggered by calcium, and regulated via ABA dependent and/or independent pathways. Hence, adaptation of plants to such stresses involves maintaining cellular homeostasis, detoxification of harmful elements and also growth alterations. Stress in general cause excess production of reactive oxygen species (ROS) and the plants overcome the same by either preventing the accumulation of ROS or by eliminating the ROS formed. Ion homeostasis includes processes such as cellular uptake, sequestration and export in conjunction with long distance transport. Requisite amounts of osmolytes are hence synthesized under stress to maintain turgor along with maintaining the macromolecular structures and also for scavenging ROS. Another noteworthy response is the accumulation of novel proteins, including enzymes involved in the biosynthesis of osmoprotectants, heat-shock proteins (HSPs), late embryogenesis abundant (LEA) proteins, antifreeze proteins, chaperones, detoxification enzymes, transcription factors, kinases and phosphatases. The LEAs belong to a redundant protein family and are highly hydrophilic, boiling-soluble, non-globular and therefore have been defined and classified accordingly. The precise function of LEAs is still unknown, but substantial evidence indicates their involvement in dessication tolerance as the expression of LEAs confers increased resistance to stress in heterologous yeast system and also significantly improves water deficit tolerance in transgenic plants. Genetic manipulation of plants towards conferring abiotic stress tolerance is a daunting task, as the abiotic stress tolerance mechanism is highly complex and various strategies have been exploited to address and evaluate the stress tolerance mechanism, and the molecular responses to water deficit via complex signaling networks. Genomic technologies have recently been useful in integrating the multigenicity of the plant stress responses through, transcriptomics, proteomics and metabolite profilling and their interactions. This review deals with the recent developments on genetic approaches for water stress tolerance in plants, with special emphasis on LEAs.

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“…Water (Ψw) and osmotic (Ψπ) potential were measured (Ling et al 1994) with leaf psychrometer L-51A connected to dewpoint microvoltmeter HR-33 T (Wescor, Logan, UT, USA). Measurements were performed at 10:00 am and 6:00 pm on the last day of growth at optimal temperature and on two consecutive days under severe cold.…”

Section: Evaluation Of Physiological Response Of Seedlings To Severe mentioning

confidence: 99%

Global analysis of gene expression in maize leaves treated with low temperature. II. Combined effect of severe cold (8 °C) and circadian rhythm

Jończyk

Sobkowiak

Trzcińska-Danielewicz

et al. 2017

Plant Mol Biol

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numerous genes lacking circadian rhythm responded to the cold by undergoing up-or down-regulation. Notably, the transcriptome changes preceded major physiological manifestations of cold stress. In silico analysis of metabolic processes likely affected by observed gene expression changes indicated major down-regulation of photosynthesis, profound and multifarious modulation of plant hormone levels, and of chromatin structure, transcription, and translation. A role of trehalose and stachyose in cold stress signaling was also suggested. Meta-analysis of published transcriptomic data allowed discrimination between general stress response of maize and that unique to severe cold. Several cis-and trans-factors likely involved in the latter were predicted, albeit none of them seemed to have a major role. These results underscore a key role of modulation of diel gene expression in maize response to severe cold and the unique character of the cold-response of the maize circadian clock.

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Alterations in Water Status, Endogenous Abscisic Acid Content, and Expression of rab18 Gene during the Development of Freezing Tolerance in Arabidopsis thaliana

Abstract: Treatments as diverse as exposure to low temperature (LT), exogenous abscisic acid (ABA)

Cited by 257 publications

References 31 publications

Sugar/osmoticum levels modulate differential abscisic acid-independent expression of two stress-responsive sucrose synthase genes in Arabidopsis

Sugar/osmoticum levels modulate differential abscisic acid-independent expression of two stress-responsive sucrose synthase genes in Arabidopsis

Genetic approaches towards overcoming water deficit in plants - special emphasis on LEAs

Global analysis of gene expression in maize leaves treated with low temperature. II. Combined effect of severe cold (8 °C) and circadian rhythm

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