Alterations of epithelial layer after ischemic preconditioning of small intestine in rats

Abstract

Ischemic-reperfusion (IR) injury of the small intestine makes a serious complications associated with various surgical procedures and is related to changes in motility, secretory activity and structural alterations. Preconditioning can reduce range of this damage. The aim of the experimental study was to determine the influence of ischemic preconditioning (IPC) on IR injury on jejunal epithelial layer. Wistar rats (n = 56) were divided in two experimental groups. IR group was subjected to 60 min ischemia of cranial mesenteric artery and followed by reperfusion periods: 1,4,8,24 h (IR1, IR4, IR8, IR24). Group with ischemic preconditioning (IPC+IR) was subjected to two subsequent ischemic attacks (12 min) with 10 min of reperfusion between them, and after 2nd attack ischemia was induced for 60 min followed by relevant reperfusion period. IPC showed the protective impact on the jejunal tissue architecture after 1 h reperfusion, when in IR1 group the highest and significant damage was observed (p < 0.001) in contrast to IPC+IR1 group. Histopathological damage of the intestine in pretreated groups was postponed to 4 h of reperfusion. Protective effect of IPC together with later accumulation of injury signs were confirmed by weaker impact on goblet cell (p < 0.001) and Paneth cell populations (p < 0.05).The increased cells proliferation in preconditioned groups came later, but stronger after 8 h of reperfusion (p < 0.001) and after 24 h of reperfusion still remained at the high activity level (p < 0.001). Our experimental results on the histopathological changes in the jejunum during ischemic preconditioning proved that IPC may have a positive effect on maintaining intestinal barrier function.

Protective Effects of Dexmedetomidine on Ischemia-Reperfusion Injury in Rat Ovary

“…In these cases ischemic damage lead to the formation of more tissue damage. 16,18,20 Therefore, in order to avoid I/R damage the use of antioxidant pharmacological agents may be useful during or before reperfusion. 20 In studies Dexmedetomidine has been shown to be useful in intestinal, testicular, kidney, lung and cardiac I/R injury.…”

“…16,18,20 Therefore, in order to avoid I/R damage the use of antioxidant pharmacological agents may be useful during or before reperfusion. 20 In studies Dexmedetomidine has been shown to be useful in intestinal, testicular, kidney, lung and cardiac I/R injury. [11][12][13][14][15] There is no study that showing the protective effect of dexmedetomidine on the I/R related tissue damage in rat ovary.…”

Does sildenafil have protective effects against ovarian ischemia-reperfusion injury in rats?

“…This increasing oxygen reacts with hypoxanthine and xanthine oxidase in ischemic tissue and finally, FOR formation, which causes tissue damage, is triggered [21]. Increasing FOR production causes a greater increase in damage in ischemic tissue by raising the peroxidation of cell membranes and mitochondrial lipids [3].…”

A combination of plasma DAO and citrulline levels as a potential marker for acute mesenteric ischemia

“…Mesenteric ischemia causes a hypoxic cell degeneration process in intestinal epithelial cells (23). This process disrupts the mucosal barrier and allows the entry of digestive enzymes into the intestinal wall, leading to autodigestion of the intestinal structure (24).…”

“…In our study, the experimental models of the short-term ischemia group and the prolonged ischemia group are different. However, the expected effect of the short ischemia is similar because the short time ischemia (15 min) causes slight intestinal injury that is not altered but is worsened insignificantly by reperfusion (23–25). This slight intestinal damage is sufficient for our comparison.…”