Altered ganglioside expression by SH-SY5Y cells upon retinoic acid-induced neuronal differentiation

Rebhan, Michael; Vacun, Gabriele; Bayreuther, Klaus; Rösner, Harald

doi:10.1097/00001756-199404000-00022

Cited by 30 publications

(32 citation statements)

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“…Increased expression of complex gangliosides is a well-described feature of normal embryonic neurogenesis and accelerated axonogenesis (Svennerholm et al, 1989;Hirschberg et al, 1996). Similar ganglioside changes to those we describe here have been reported in retinoic acid-treated Xenopus embryo cells, murine embryonal carcinoma cells and SHSY-5Y NB cells during neuronal maturation (Rebhan et al, 1994;Rizzo et al, 1995;Liour et al, 2000). Thus, predominant expression of complex gangliosides can be considered a biochemical marker of increasing neuronal differentiation.…”

Section: Discussionsupporting

confidence: 87%

“…However, 13-cis RA isomerises in vivo to both ATRA and 9-cis retinoic acid, which are believed to be the active compounds in vivo in nonocular tissue (Lovat et al, 1994). Since retinoic acid treatment has been associated with changes in the ganglioside complement of various cell types (Li and Ladisch, 1992;Rebhan et al, 1994;Liour et al, 2000), and the oral maintenance therapy of disseminated NB with 13-cis RA results in improved event-free survival (Matthay et al, 1999), we sought to specifically explore the effect of retinoic acid on CbG expression. We, therefore, first treated NB cells in vitro with 10 mM ATRA, then evaluated ATRA-induced changes in the composition of individual gangliosides and in total cellular ganglioside content in LAN-1, LAN-5, SMS-KCNR and SMS-KCN cells (Table 2).…”

Section: Retinoic-acid Induced Changes In Cbg Expression In Nb Cell Lmentioning

confidence: 99%

“…Previous in vitro studies have shown that morphological signs of neuronal differentiation in response to retinoic acid are accompanied by an increase in total ganglioside content and a relative increase in the expression of certain complex gangliosides of both the 'a' and 'b' pathway in NB cells (Li and Ladisch, 1992;Rebhan et al, 1994), as well as in normal and transformed embryonal cells (Levine and Flynn, 1986;Rizzo et al, 1995;Liour et al, 2000). On the basis of these findings, we have undertaken a comprehensive analysis of retinoic acid-induced changes in ganglioside expression and metabolism in human NB cell lines.…”

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confidence: 99%

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Alterations in neuroblastoma ganglioside synthesis by induction of GD1b synthase by retinoic acid

et al. 2004

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Recent findings link increased expression of the structurally complex 'b' pathway gangliosides GD1b, GT1b, GQ1b (CbG) to a favourable clinical and biological behaviour in human neuroblastoma (NB). Seeking a model to probe these observations, we evaluated four human NB cell lines. Very low CbG content (4 -10%) in three of the four cell lines (LAN-5, LAN-1, SMS-KCNR) reflected the ganglioside pattern observed in the most aggressive NB tumours. Pharmacological alterations of complex ganglioside synthesis in vitro by a 5 -7 day exposure to 5 -10 mM retinoic acid, which is employed in maintenance therapy of disseminated NB, included markedly increased (i) relative expression of CbG (6.672.0-fold increase, P ¼ 0.037), (ii) relative expression of the analogous 'a' pathway gangliosides, termed CaG (6.471.4-fold increase in GM1a and GD1a; P ¼ 0.010), and (iii) total cellular ganglioside content (2.0 -6.3-fold), which in turn amplified the accumulation of structurally complex gangliosides. Substantial increases (2.7 -2.9-fold) in the activity of GD1b/GM1a synthase (b-1,3-galactosyltransferase), which initiates the synthesis of CbG and CaG, accompanied the all-trans retinoic acid (ATRA)-induced ganglioside changes. Thus, increased CbG synthesis in NB cell lines is attributable to a specific effect of ATRA, namely induction of GD1b/GM1a synthase activity. Since the shift towards higher expression of CbG and CaG during retinoic acid-induced cellular differentiation reflects a ganglioside pattern found in clinically less-aggressive tumours, our studies suggest that complex gangliosides may play a role in the biological and clinical behaviour of NB.

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Section: Discussionsupporting

confidence: 87%

Section: Retinoic-acid Induced Changes In Cbg Expression In Nb Cell Lmentioning

confidence: 99%

mentioning

confidence: 99%

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Alterations in neuroblastoma ganglioside synthesis by induction of GD1b synthase by retinoic acid

et al. 2004

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“…2, lane 6). This result again complies with the endogenous glycolipid pattern (12). We could also observe that the glycosylation products found in the incubation media were the same as those found in the respective cell pellets.…”

Section: Uptake Of Labeled Glucosylthioceramides By Culturedsupporting

confidence: 86%

“…Human neuroblastoma-derived SHSY5Y cells were grown as described (12). All cells were grown at 37°C in a water-saturated atmosphere of 5% CO 2 in air.…”

Section: Methodsmentioning

confidence: 99%

Demonstration of Direct Glycosylation of Nondegradable Glucosylceramide Analogs in Cultured Cells

Schwarzmann

Hofmann

Pütz

et al. 1995

Journal of Biological Chemistry

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After uptake by various cells (human skin fibroblasts, rat neuroblastoma B 104, human neuroblastoma SHSY5Y, murine cerebellar cells), a radioactive and a fluorescent analog of a nondegradable glucosylceramide with sulfur in the glycosidic link were glycosylated to a cell-specific pattern of glycolipid analogs. These results, for the first time, show that a glucosylceramide analog can be conveyed from the plasma membrane of cultured cells to those Golgi compartments that function in the early glycosylation steps of glycolipids. This observation is further confirmed by the fact that the cationic ionophore monensin, known to impede membrane flow from proximal to distal Golgi cisternae, inhibited the formation of complex ganglioside analogs but not those of lactosylceramide, sialyl lactosylceramide (G M3 ), and disialyl lactosylceramide (G D3 ).

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Retinoic acid‐induced changes in polysialyltransferase mRNA expression and NCAM polysialylation in human neuroblastoma cells

Seidenfaden

Hildebrandt

2000

J. Neurobiol.

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Altered ganglioside expression by SH-SY5Y cells upon retinoic acid-induced neuronal differentiation

Cited by 30 publications

References 0 publications

Alterations in neuroblastoma ganglioside synthesis by induction of GD1b synthase by retinoic acid

Alterations in neuroblastoma ganglioside synthesis by induction of GD1b synthase by retinoic acid

Demonstration of Direct Glycosylation of Nondegradable Glucosylceramide Analogs in Cultured Cells

Retinoic acid‐induced changes in polysialyltransferase mRNA expression and NCAM polysialylation in human neuroblastoma cells

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