Altered Gene Expression in Gingival Tissues and Enhanced Bone Loss in Rats With Diabetes With Experimental Periodontitis

Soboku, Kyoko; Kikuchi, Takeshi; Fujita, Sachiko; Takeda, Hiroaki; Naruse, Keiko; Noguchi, Toshihide

doi:10.1902/jop.2013.120705

Cited by 13 publications

(17 citation statements)

References 51 publications

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“…Distinct animal experimentation protocols were used in the reviewed studies. The murine experimental models were used in 100% of the studies, from which the mouse model was the most frequently used animal model (76.2%) . In the studies using mice, 10 studies used the C57BL/6 strain, four studies used the BALB/c strain, and three studies did not specify the animal strain used .…”

Section: Resultsmentioning

confidence: 99%

“…From them, only eight studies reported the number of mice that were analysed (M = 22.6, SD = 8.30, total = 181 mice). In the studies using rats, three studies used the Wistar strain, two studies used the Sprague Dawley strain, and one study used the Zucker Diabetic Fat strain . All the studies reported the number of rats that were analysed (M = 33.3, SD = 7.76, total = 200 rats).…”

Section: Resultsmentioning

confidence: 99%

“…Distinct animal experimentation protocols were used in the reviewed studies. The murine experimental models were used in 100% of the studies, 3,8,[12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30] from which the mouse model was the most frequently used animal model (76.2%). 3,[12][13][14][15][16][17][18][19][20][21][22][23]25,26,28,30 In the studies using mice, 10 studies used the C57BL/6 strain, 3,12,[14][15][16][17][21][22][23]26 four studies used the BALB/c strain, 13,18,…”

Section: Methodological Heterogeneitymentioning

confidence: 99%

“…In the studies using rats, three studies used the Wistar strain, 24,27,29 two studies used the Sprague Dawley strain, 8,30 and one study used the Zucker Diabetic Fat strain. 20 All the studies reported the number of rats that were analysed (M = 33.3, SD = 7.76, total = 200 rats). Only one study 21 used also beagle dogs, without reporting the number of animals used (Tables S2 and S4).…”

Section: Methodological Heterogeneitymentioning

confidence: 99%

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The therapeutic potential of regulatory T lymphocytes in periodontitis: A systematic review

Cafferata

Jerez

Vernal

et al. 2018

J of Periodontal Research

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This systematic review aimed to: (a) generate a descriptive synthesis of preclinical studies assessing the therapeutic potential of regulatory T lymphocytes (Tregs) to arrest periodontitis, (b) evaluate the methodological heterogeneity of the reviewed animal studies and (c) assess the risk of bias (RoB) of the included studies. The electronic search for animal studies included the MEDLINE, EMBASE, Web of Science and LILACS databases. In addition, a manual search assessed the high‐ranked scientific journals in “periodontics/immunology” and the references listed in the included studies. There were no language, year or publication status restrictions. Two independent reviewers selected and extracted the data, and Cohen's Kappa coefficient was calculated to determine the inter‐examiner agreement. The Systematic Review Center for Laboratory Animal Experimentation's (SYRCLE) tool was used to assess the RoB. A total of 21 of the 425 studies obtained from the database search were included. Treg function was mainly described in Porphyromonas gingivalis‐induced periodontitis (57.1%) in mice (76.2%), where Treg suppression was strongly related to disease progression and Treg induction was strongly related to immuno‐inflammatory response reduction. Of those 21 studies, eight included eight animal experiments using three distinct therapeutic approaches, including: P. gingivalis‐driven immunization (n = 3), retinoic acid inoculation (n = 2) and anti‐inflammatory molecules in polymeric carriers (n = 3), which could modulate the Treg activity through cytokine production (interleukin‐10 and transforming growth factor‐β1), CC‐chemokine‐ and CC‐chemokine receptor‐mediated chemoattraction (CCL22 and CCR4) or Th17‐associated receptor activator of nuclear factor κB ligand (RANKL) downregulation. However, the studies with animal experiments did not specify the randomization sequences and housing conditions that were used, and therefore, 42.11% of the entries were rated as unclear RoB. Distinct therapeutic strategies involving Tregs could potentially suppress the immuno‐inflammatory response and restore alveolar bone homeostasis during periodontitis. Nevertheless, important methodological variability, poor reporting of treatment effect estimates and unclear RoB suggest using caution when assessing the results of these studies.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodological Heterogeneitymentioning

confidence: 99%

Section: Methodological Heterogeneitymentioning

confidence: 99%

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The therapeutic potential of regulatory T lymphocytes in periodontitis: A systematic review

Cafferata

Jerez

Vernal

et al. 2018

J of Periodontal Research

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“…Ligature-induced periodontitis is a method to produce an animal model of periodontitis that is similar to clinical periodontitis since it results from plaque accumulation around the ligature23. Acute inflammation arises within 2 days post surgery, beginning around the cervical area and leading to a chronic inflammatory response, eventually ending in alveolar bone loss242526.…”

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confidence: 99%

Periodontitis-activated monocytes/macrophages cause aortic inflammation

Miyajima

Naruse

Kobayashi

et al. 2014

Sci Rep

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A relationship between periodontal disease and atherosclerosis has been suggested by epidemiological studies. Ligature-induced experimental periodontitis is an adequate model for clinical periodontitis, which starts from plaque accumulation, followed by inflammation in the periodontal tissue. Here we have demonstrated using a ligature-induced periodontitis model that periodontitis activates monocytes/macrophages, which subsequently circulate in the blood and adhere to vascular endothelial cells without altering the serum TNF-α concentration. Adherent monocytes/macrophages induced NF-κB activation and VCAM-1 expression in the endothelium and increased the expression of the TNF-α signaling cascade in the aorta. Peripheral blood-derived mononuclear cells from rats with experimental periodontitis showed enhanced adhesion and increased NF-κB/VCAM-1 in cultured vascular endothelial cells. Our results suggest that periodontitis triggers the initial pathogenesis of atherosclerosis, inflammation of the vasculature, through activating monocytes/macrophages.

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Single-cell transcriptional profiling reveals immunomodulatory properties of stromal and epithelial cells in periodontal immune milieu with diabetes in rats

Zhang

Zhu

Liu

et al. 2023

International Immunopharmacology

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Altered Gene Expression in Gingival Tissues and Enhanced Bone Loss in Rats With Diabetes With Experimental Periodontitis

Abstract: The results of this study indicate that the role of DM in modulating bone breakdown related to periodontitis may involve an increased level of LBP and reduced levels of T-helper 2 cytokines.

Cited by 13 publications

References 51 publications

The therapeutic potential of regulatory T lymphocytes in periodontitis: A systematic review

The therapeutic potential of regulatory T lymphocytes in periodontitis: A systematic review

Periodontitis-activated monocytes/macrophages cause aortic inflammation

Single-cell transcriptional profiling reveals immunomodulatory properties of stromal and epithelial cells in periodontal immune milieu with diabetes in rats

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