Altered Gene Expression Profiles of Wheat Genotypes against Fusarium Head Blight

Ayumi, Kosaka; Manickavelu, Alagu; Kajihara, Daniela; Nakagawa, Hiroyuki; Ban, Tomohiro

doi:10.3390/toxins7020604

Cited by 25 publications

(24 citation statements)

References 35 publications

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“…Several studies have shown that acidic chitinases are induced by salicylic acid (Brederode et al 1991, Buchter et al 1997, Ancillo et al 1999. In agreement with our study, Lawton et al (1994) showed that in cucumber, the expression of class III Cht increases 10-fold following an application of SA. In our study, application of SA increased Cht-4 gene expression in both the cultivars.…”

Section: Discussionsupporting

confidence: 93%

“…In plants several genes such as ABC transporters, multidrug resistance-associated proteins, and PDRs play major roles in detoxification processes (Kosaka et al 2015). Pleiotropic drug-resistant protein transporters have been shown to be involved in plant defense, formation of the cuticular layer, and transport of antimicrobial terpenoids (Kang et al 2010).…”

Section: Discussionmentioning

confidence: 99%

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Application of sodium salicylate up-regulates defense responseagainst Fusarium graminearum in wheat spikes

Sorahinobar¹,

Niknam²,

Jahedi³

et al. 2019

Biol. plant.

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Fusarium head blight caused by the hemibiotrophic fungus Fusarium graminearum is one of the most devastating diseases of wheat which reduces both grain yield and quality. To better understand mechanism underlying wheat resistance to this pathogen, the expressions of five candidate genes encoding phenylalanine ammonia-lyase (PAL), glucanase-2 (Gl 2), class IV chitinase (Cht-4), cytochrome P450 (CYP), and pleiotropic drug resistance (PDR) following spike inoculation with F. graminearum was compared in susceptible cv. Falat and resistant cv. Sumai3 at three time points (48, 96, 144 h after inoculation). Real-time quantitative PCR analysis indicated earlier and greater inductions of PAL, Glu-2, and Cht-4 in spikes of 'Sumai3' as compared to 'Falat' in response to F. graminearum inoculation. The expression of CYP in the resistant 'Sumai3' was about three times higher than in 'Falat' at 144 h after pathogen inoculation. Moreover, soil drench application of sodium salicylate (SA) one day before pathogen inoculation drastically curtailed pathogen infection in both the cultivars. Furthermore, SA treatment caused an induction of these genes in spikes of the susceptible cultivar to show a similar pattern as in the resistant one when inoculated with F. graminearum. Proteomics analysis of F. graminearum treated spikes 96 h after inoculation confirmed an increase of Glu and Cht spot volume in 'Sumai3' whereas a decrease in 'Falat'. The SA treatment also caused significant increases in Glu and Cht spot volumes in both the cultivars. Our findings show an association between SA improvement of wheat defense against F. graminearum infection and induction of genes encoding proteins involved in pathogen response (Glu-2, Cht-4), secondary metabolite biosyntheses (PAL), and xenobiotic detoxification (CYP and PDR).

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Application of sodium salicylate up-regulates defense responseagainst Fusarium graminearum in wheat spikes

Sorahinobar¹,

Niknam²,

Jahedi³

et al. 2019

Biol. plant.

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“…Thirty-seven transcripts (most with increased abundance 18 DPI – Supplementary Figure S5R) belonging to three multidrug transporter superfamilies comprised MATE eﬄux proteins, MFS proteins, and ABC transporters. The importance of these group of genes is exemplified by plant ABC transporters, a large family of ATP-driven pumps that aids in secondary metabolite transport to deter pathogens, and that can be used for detoxification of harmful compounds (fungal toxins; Yazaki, 2006; Kang et al, 2011; Kosaka et al, 2015). …”

Section: Resultsmentioning

confidence: 99%

RNA-seq Transcriptome Response of Flax (Linum usitatissimum L.) to the Pathogenic Fungus Fusarium oxysporum f. sp. lini

Galindo‐González

Deyholos

2016

Front. Plant Sci.

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Fusarium oxysporum f. sp. lini is a hemibiotrophic fungus that causes wilt in flax. Along with rust, fusarium wilt has become an important factor in flax production worldwide. Resistant flax cultivars have been used to manage the disease, but the resistance varies, depending on the interactions between specific cultivars and isolates of the pathogen. This interaction has a strong molecular basis, but no genomic information is available on how the plant responds to attempted infection, to inform breeding programs on potential candidate genes to evaluate or improve resistance across cultivars. In the current study, disease progression in two flax cultivars [Crop Development Center (CDC) Bethune and Lutea], showed earlier disease symptoms and higher susceptibility in the later cultivar. Chitinase gene expression was also divergent and demonstrated and earlier molecular response in Lutea. The most resistant cultivar (CDC Bethune) was used for a full RNA-seq transcriptome study through a time course at 2, 4, 8, and 18 days post-inoculation (DPI). While over 100 genes were significantly differentially expressed at both 4 and 8 DPI, the broadest deployment of plant defense responses was evident at 18 DPI with transcripts of more than 1,000 genes responding to the treatment. These genes evidenced a reception and transduction of pathogen signals, a large transcriptional reprogramming, induction of hormone signaling, activation of pathogenesis-related genes, and changes in secondary metabolism. Among these, several key genes that consistently appear in studies of plant-pathogen interactions, had increased transcript abundance in our study, and constitute suitable candidates for resistance breeding programs. These included: an induced RPMI-induced protein kinase; transcription factors WRKY3, WRKY70, WRKY75, MYB113, and MYB108; the ethylene response factors ERF1 and ERF14; two genes involved in auxin/glucosinolate precursor synthesis (CYP79B2 and CYP79B3); the flavonoid-related enzymes chalcone synthase, dihydroflavonol reductase and multiple anthocyanidin synthases; and a peroxidase implicated in lignin formation (PRX52). Additionally, regulation of some genes indicated potential pathogen manipulation to facilitate infection; these included four disease resistance proteins that were repressed, indole acetic acid amido/amino hydrolases which were upregulated, activated expansins and glucanases, amino acid transporters and aquaporins, and finally, repression of major latex proteins.

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“…toxins and enzymes), was higher in the FHB‐resistant wheat genotypes Dream and Sumai 3 than in the FHB‐susceptible cultivar Lynx (Gottwald et al ., ). Similar categories of genes differentially and/or temporally regulated between partially FHB‐resistant and FHB‐susceptible wheat genotypes have also been reported by other transcriptomic studies (Kosaka et al ., ; Muhovski et al ., ).…”

Section: How the Host Plant Defends Itself Against F Graminearum: Trmentioning

confidence: 98%

Transcriptomics of cereal–Fusarium graminearum interactions: what we have learned so far

Kazan

Gardiner

2017

Molecular Plant Pathology

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The ascomycete fungal pathogen Fusarium graminearum causes the globally important Fusarium head blight (FHB) disease on cereal hosts, such as wheat and barley. In addition to reducing grain yield, infection by this pathogen causes major quality losses. In particular, the contamination of food and feed with the F. graminearum trichothecene toxin deoxynivalenol (DON) can have many adverse short- and long-term effects on human and animal health. During the last decade, the interaction between F. graminearum and both cereal and model hosts has been extensively studied through transcriptomic analyses. In this review, we present an overview of how such analyses have advanced our understanding of this economically important plant-microbe interaction. From a host point of view, the transcriptomes of FHB-resistant and FHB-susceptible cereal genotypes, including near-isogenic lines (NILs) that differ by the presence or absence of quantitative trait loci (QTLs), have been studied to understand the mechanisms of disease resistance afforded by such QTLs. Transcriptomic analyses employed to dissect host responses to DON have facilitated the identification of the genes involved in toxin detoxification and disease resistance. From the pathogen point of view, the transcriptome of F. graminearum during pathogenic vs. saprophytic growth, or when infecting different cereal hosts or different tissues of the same host, have been studied. In addition, comparative transcriptomic analyses of F. graminearum knock-out mutants with altered virulence have provided new insights into pathogenicity-related processes. The F. graminearum transcriptomic data generated over the years are now being exploited to build a systems level understanding of the biology of this pathogen, with an ultimate aim of developing effective and sustainable disease prevention strategies.

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Altered Gene Expression Profiles of Wheat Genotypes against Fusarium Head Blight

Cited by 25 publications

References 35 publications

Application of sodium salicylate up-regulates defense responseagainst Fusarium graminearum in wheat spikes

Application of sodium salicylate up-regulates defense responseagainst Fusarium graminearum in wheat spikes

RNA-seq Transcriptome Response of Flax (Linum usitatissimum L.) to the Pathogenic Fungus Fusarium oxysporum f. sp. lini

Transcriptomics of cereal–Fusarium graminearum interactions: what we have learned so far

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