2009
DOI: 10.1016/j.imlet.2009.02.009
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Altered hepatic inflammatory response in the offspring following prenatal LPS exposure

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Cited by 16 publications
(22 citation statements)
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“…RT-PCR is a technique that has been successfully used by many studies examining the expression levels of many inflammatory mediators at the mRNA level [30,[42][43][44][45]; however, subtle differences in the expression of these neuroinflammatory mediators may fail to be detected by RT-PCR due to its lower sensitivity compared to other techniques such as real-time PCR.…”
Section: Discussionmentioning
confidence: 99%
See 3 more Smart Citations
“…RT-PCR is a technique that has been successfully used by many studies examining the expression levels of many inflammatory mediators at the mRNA level [30,[42][43][44][45]; however, subtle differences in the expression of these neuroinflammatory mediators may fail to be detected by RT-PCR due to its lower sensitivity compared to other techniques such as real-time PCR.…”
Section: Discussionmentioning
confidence: 99%
“…After cDNA synthesis, PCR amplification was carried out using appropriate sense and antisense primers specific for rat b-actin (a housekeeping gene), IL-1b, IL-6, TNF-a, KC, MIP-1b, MCP-1, TLR-4, CD14, and Myd88 in a final volume of 20 ll containing 1 ll of cDNA, 19 PCR buffer, 0.2 lM of each sense and antisense primer, 0.2 mM of dNTPs, and 1 U of Taq DNA polymerase (Promega). The information on specific primers and PCR conditions for b-actin, IL-1b, IL-6, TNF-a, KC, MIP-1b, TLR-4, and CD14 is described in Lasala et al [19] and Surriga et al [30]. The sense and antisense primers for rat Myd88 were 5 0 -TGT-CCC-AAA-GGA-AAC-ACA-CA-3 0 and 5 0 -CCC-CTG-ACA-TGC-CTA-TCA-CT-3 0 , respectively, and the sense and antisense primers for rat MCP-1 were 5 0 -ATG-ATC-CCA-ATG-AGT-CGG-3 0 and 5 0 -ACA-GAA-GTG-CTT-GAG-GTG-3 0 , respectively.…”
Section: Total Rna Extractionmentioning
confidence: 99%
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“…The results reported by Urakubo et al (2001) show that maternal exposure to infection alters the inflammatory cytokine levels in the foetal brain. Lipopolysaccharide (LPS) stimulation during pregnancy alters the immune response in developing offspring (Surriga et al 2009). Reduced litter size, abnormal foetal development and decreased fertility have been major problems associated with livestock production in China.…”
Section: Introductionmentioning
confidence: 99%