Altered microRNA expression profiles in lung damage induced by nanosized SiO₂

Abstract

The objective of the present research is to explore miRNAs expression profiles in lung tissue of rat treated by nanosized SiO 2 in the light of normal at diverse dosages, time, predict their target genes, and probe the biological function and regulation of miRNA in the lung damage process caused by nanosized SiO 2 . Up-regulation of rno-miR-208, rno-miR-212 and rno-miR-18a in lung tissue mainly characterized by inflammation of SD rats caused by nanosized SiO 2 particles instilled intratracheally at 7 th , 15 th 30 th d using Illumina HiSeq2000 sequencing technique and were further verified by quantitative reverse transcriptase polymerase chain reaction (qRT PCR) assay. Lung damage is mainly with characteristics of lung interstitial fibrosis, upregulation of rno-miR-212, rno-miR-144, rno-miR-702-3p, rno-miR-379 and rno-miR-127, down-regulation of rno-miR-541 at 60 th , 90 th d post-exposure. As target genes of rno-miR-208, rno-miR-212 and rno-miR-18a respectively, there was no statistical significance of programmed cell death 4 (PDCD4), LIN28B and connective tissue growth factor (CTGF) mRNA expression level (P > 0.05) compared to b-actin as internal controls detected by Real-time quantitative PCR. The differences in protein gray value ratio of PDCD4, LIN28B and CTGF detected by Western blotting test were statistically significant (P < 0.05). These results suggested that miR-208, miR-212 and miR-18a may take effects in rats' lung damage lead by nanosized SiO 2 . Their target genes of PDCD4, LIN28B and CTGF functioned in translation level of target genes in regulation of inflammatory signaling pathways and involved in the formation of tissue fibrosis.

MiR‐144‐induced KLF2 inhibition and NF‐kappaB/CXCR1 activation promote neutrophil extracellular trap–induced transfusion‐related acute lung injury

“…Similarly, a previous study has demonstrated the presence of highly expressed miR‐144 in ALI 32 . Additionally, one report focusing on miRNA expression pattern in nanosized SiO‐induced lung damage was found to be accompanied by the up‐regulation of miR‐144 33 …”

“… 32 Additionally, one report focusing on miRNA expression pattern in nanosized SiO‐induced lung damage was found to be accompanied by the up‐regulation of miR‐144. 33 …”

“…found to be accompanied by the up-regulation of miR-144 33. Furthermore, miRNAs can regulate an expression of a target gene at the transcriptional and post-transcriptional level by binding to the target mRNA part and participating in the entire pathogenesis of ALI.…”

LncRNA CASC2 is involved in the development of chronic obstructive pulmonary disease via targeting miR-18a-5p/IGF1 axis

“…The dysregulation of miR-18a-5p has been reported to participate in several respiratory diseases. 14 , 15 miR-18a-5p can target the glucocorticoid receptor, which is involved in the regulation of inflammatory response and related physiological events. 29 In the current study, the gain and loss function experiments revealed that upregulation of miR-18a-5p aggravated CSE-induced cell apoptosis and inflammatory cytokines release.…”

“… 14 Another study also reported the upregulation of miR-18a-5p during the lung damage process caused by nanosized SiO 2 . 15 However, the role and underlying mechanism of miR-18a-5p in COPD remain unknown.…”