Altered vitamin D metabolic system in follicular cysts of sows

Grzesiak, Małgorzata; Socha, Magdalena; Hrabia, Anna

doi:10.1111/rda.13867

Cited by 4 publications

(3 citation statements)

References 12 publications

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“…Total protein extraction and Western blot analysis were conducted as previously described [ 10 , 35 ]. Samples were separated by 10% SDS–PAGE (Mini-Protean TGX Precast Gels; Bio-Rad Laboratories Inc., GmbH, Munchen, Germany) and electroblotted onto a PVDF membrane (Trans-Blot Turbo Mini 0.2 µm PVDF Transfer Packs; Bio-Rad Laboratories Inc.) using a semi–dry Trans-Blot Turbo Transfer System (Bio-Rad Laboratories Inc.).…”

Section: Methodsmentioning

confidence: 99%

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Vitamin D3 Metabolic Enzymes in the Porcine Uterus: Expression, Localization and Autoregulation by 1,25(OH)2D3 In Vitro

Grzesiak

Kamińska

Bodzioch

et al. 2022

IJMS

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The role of vitamin D3 has been confirmed in female reproductive organs. This study aimed to examine vitamin D3 metabolic enzymes, i.e., CYP27B1 and CYP24A1, mRNA transcript and protein abundance, and protein localization in the uterus of pigs on days 2–5, 10–12, 15–16 and 18–20 of the estrous cycle. Additionally, we determined 1,25(OH)2D3 concentration in uterine flushings and the effect of 1,25(OH)2D3 (10, 50 and 100 ng/mL) in vitro on CYP27B1 and CYP24A1 mRNA transcript abundance in endometrial and myometrial slices. In the endometrium, a greater CYP27B1 mRNA transcript abundance was noted on days 10–12 and 18–20 than on days 15–16, whereas encoded protein abundance was greater on days 18–20 when compared to days 15–16. Endometrial CYP24A1 mRNA transcript abundance was greater on days 18–20 than on days 10–12 and 15–16. In the myometrium, CYP27B1 mRNA transcript abundance was greater on days 18–20 than on days 2–5 and 15–16, while protein abundance was larger in slices collected on days 18–20 than on days 15–16. Neither CYP24A1 mRNA transcript nor encoded protein abundance were detected in the myometrium. The highest 1,25(OH)2D3 concentration in uterine flushings was observed on days 18–20. Furthermore, the 1,25(OH)2D3 increased the abundance of the CYP24A1 mRNA transcript in endometrial slices. Overall, our results suggest that porcine uterus is an extra-renal site of vitamin D3 metabolism. Both the endometrium and the myometrium possess the ability to synthesize vitamin D3, while only the endometrium contributes to its catabolism.

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Section: Methodsmentioning

confidence: 99%

“…Indeed, vitamin D 3 receptor (VDR)- and 1α-hydroxylase-deficient mice were infertile, exhibited uterine hypoplasia and impaired folliculogenesis [ 5 , 6 ]. In addition, disrupted vitamin D 3 metabolism was described in reproductive pathologies such as ovarian cysts, endometriosis and uterine leiomyoma [ 7 , 8 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Vitamin D3 Metabolic Enzymes in the Porcine Uterus: Expression, Localization and Autoregulation by 1,25(OH)2D3 In Vitro

Grzesiak

Kamińska

Bodzioch

et al. 2022

IJMS

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“…Besides the best known regulation of calcium uptake, it is an essential regulator of reproductive processes in humans and animals (for review see [8,16,17]). Insufficiency/deficiency of vitamin D 3 contributes to serious reproductive disturbances [18,19]. For example, hens fed a vitamin D 3 -deficient diet frequently lay thin-shelled and soft-shelled eggs [18].…”

Section: Introductionmentioning

confidence: 99%

Vitamin D3 Receptors and Metabolic Enzymes in Hen Reproductive Tissues

Hrabia,

Kamińska,

Socha

et al. 2023

IJMS

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In recent years, vitamin D3 has been revealed as an important regulator of reproductive processes in humans and livestock; however, its role in the female reproductive system of poultry is poorly known. The aim of this study was to examine vitamin D3 receptor (VDR and PDIA3) and metabolic enzyme (1α-hydroxylase and 24-hydroxylase) mRNA transcript and protein abundances, and protein localization within the hen ovary, oviductal shell gland, pituitary, liver, and kidney. We demonstrated, for the first time, the patterns of the relative mRNA and protein abundances of examined molecules in the ovary, dependent on follicle development and the layer of follicle wall, as well as in other examined organs. Immunohistochemically, PDIA3, 1α-hydroxylase, and 24-hydroxylase are localized in follicular theca and granulosa layers, luminal epithelium and tubular glands of the shell gland, pituitary, liver, and kidney. These results indicate that reproductive tissues have both receptors, VDR, primarily involved in genomic action, and PDIA3, probably participating in the rapid, non-genomic effect of vitamin D3. The finding of 1α-hydroxylase and 24-hydroxylase expression indicates that the reproductive system of chickens has the potential for vitamin D3 synthesis and inactivation, and may suggest that locally produced vitamin D3 can be considered as a significant factor in the orchestration of ovarian and shell gland function in hens. These results provide a new insight into the potential mechanisms of vitamin D3 action and metabolism in the chicken ovary and oviduct.

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