Altererythrobacter muriae sp. nov., isolated from hypersaline Añana Salt Valley spring water, a continental thalassohaline-type solar saltern

Abstract

A novel salt-tolerant alpha-proteobacterium, designated SALINAS58T, was isolated from Santa Engracia hypersaline spring water in the Añana Salt Valley, Álava, Spain. The isolate was Gram-negative, aerobic, non-motile, catalase-positive, oxidase-negative, rod-shaped and formed orange colonies on marine agar. Optimal growth was observed at pH 6.0–6.5, at 30 °C and in the presence of 1% (w/v) NaCl. The main cellular fatty acids (>20%) were summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c) and summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c). The major respiratory quinone was ubiquinone Q-10 and the major polar lipids detected were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidilglycerol, four unidentified glycolipids and one unidentified phospholipid. Strain SALINAS58T had the highest 16S rRNA gene sequence similarity to Altererythrobacter marensis MSW-14T (96.6%), Altererythrobacter aquaemixtae JSSK-8T (96.5%) and Pontixanthobacter luteolus SW-109T (96.5%) followed by Altererythrobacter atlanticus 26DY36T (96.4%). Results of the phylogenetic analysis, based on 16S rRNA gene sequences, and phylogenetic approaches based on whole genome nucleotide differences, showed that strain SALINAS58T could be distinguished from recognized species of the genus Altererythrobacter . The genomic DNA G+C content was 61.4 mol%. Digital DNA–DNA hybridization, average nucleotide identity and average aminoacid identity values between the genome of strain SALINAS58T and A. marensis MSW-14T were 18.4, 73.1 and 68.1%, respectively. Based on data from this polyphasic characterization, strain SALINAS58T (=CECT 30029T=LMG 31726T) is considered to be classified as representing a novel species in the genus Altererythrobacter , for which the name Altererythrobacter muriae sp. nov. is proposed.

Altererythrobacter lutimaris sp. nov., a marine bacterium isolated from a tidal flat and reclassification of Altererythrobacter deserti, Altererythrobacter estronivorus and Altererythrobacter muriae as Tsuneonella deserti comb. nov., Croceicoccus estronivorus comb. nov. and Alteripontixanthobacter muriae comb. nov.

“…The description is the same as for A. muriae [41]. Genome phylogenomic analysis strongly supported the placement of this species in the genus Pontixanthobacter .…”

Ancient saltern metagenomics: tracking changes in microbes and their viruses from the underground to the surface

“…BLAST analyses (data not shown) of operational phylogenetic units (OPUs), accounting for >1% of the sequences in each sample, indicated that the OPUs were closely related to sequences retrieved from other hypersaline environments worldwide, either terrestrial or aquatic. Very recently, a new species of Alpha‐proteobacteria ( Altererythrobacter muriae ) has been isolated from the SE spring (Muniozguren et al ., 2021). In our dataset this bacterium corresponded to OPU 114 (Supplementary Dataset 1) that was detected, albeit at very low abundance, in HU, as also indicated by recruiting the reads from the four cellular metagenomes generated in this work (data not shown) to A .…”

Diversity of prokaryotic microorganisms in alkaline saline soil of the Qarhan Salt Lake area in the Qinghai–Tibet Plateau

“…As a result of new technologies such as fluorescence in situ hybridization (FISH) and metagenomics, life can now be detected in the most extreme environments. Among these, the study of microorganisms in hypersaline environments such as solar salterns 6 , 7 , saltpans 8 , salt mines 9 , oceans 5 , 10 , and salt lakes 11 – 16 has received considerable interest. Microorganisms that have adapted to life at high salt concentrations are found in three domains of life: Archaea, Bacteria, and Eukarya 4 , 13 , 17 – 22 .…”