Alternating electric fields combined with activated carbon for disinfection of Gram negative and Gram positive bacteria in fluidized bed electrode system

Racyte, Justina; Bernard, Séverine; Paulitsch-Fuchs, Astrid H.; Yntema, Doekle; Brüning, H.; Rijnaarts, H.H.M.

doi:10.1016/j.watres.2013.08.011

Cited by 14 publications

(7 citation statements)

References 37 publications

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“…The dynamics of time-dependent LRF decline differed for each microorganism. Intermittent current delivery may result in interruption of cell division and cell stress as electrical current is variously applied (10,11). The different disc and electrode combinations studied exhibited a significant electricidal effect for all combinations studied, except for S. epidermidis grown on Teflon discs with titanium electrodes, the last possibly due to the small sample size tested.…”

Section: Discussionmentioning

confidence: 96%

Antibiofilm Activity of Low-Amperage Continuous and Intermittent Direct Electrical Current

Schmidt-Malan

Karau

Cede

et al. 2015

Antimicrob Agents Chemother

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Bacterial biofilms are difficult to treat using available antimicrobial agents, so new antibiofilm strategies are needed. We previously showed that 20, 200, and 2,000 A of electrical current reduced bacterial biofilms of Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa. Here, we tested continuous direct current at lower amperages, intermittent direct current, and combinations of surface materials (Teflon or titanium) and electrode compositions (stainless steel, graphite, titanium, or platinum) against S. aureus, S. epidermidis, and P. aeruginosa biofilms. In addition, we tested 200 or 2,000 A for 1 and 4 days against biofilms of 33 strains representing 13 species of microorganisms. The logarithmic reduction factor was used to measure treatment effects. Using continuous current delivery, the lowest active amperage was 2 A for 1, 4, or 7 days against P. aeruginosa and 5 A for 7 days against S. epidermidis and S. aureus biofilms. Delivery of 200 A for 4 h a day over 4 days reduced P. aeruginosa, S. aureus, and S. epidermidis biofilms on Teflon or titanium discs. A reduction of P. aeruginosa, S. aureus, and S. epidermidis biofilms was measured for 23 of 24 combinations of surface materials and electrode compositions tested. Four days of direct current delivery reduced biofilms of 25 of 33 strains studied. In conclusion, low-amperage current or 4 h a day of intermittent current delivered using a variety of electrode compositions reduced P. aeruginosa, S. aureus, and S. epidermidis biofilms on a variety of surface materials. The electricidal effect was observed against a majority of bacterial species studied.C hronic infections associated with medical devices such as joint replacements and other types of orthopedic instrumentation, prosthetic heart valves, pacemakers, implantable defibrillators, urinary catheters and stents, peritoneal dialysis catheters, intravascular catheters, cerebrospinal fluid shunts, breast implants, and vascular grafts and stents are common in today's medical practice. When these devices become infected, they must often be removed to successfully cure the associated infection. Device removal is associated with significant morbidity, cost, and, in some cases, mortality. Prosthetic joint removal, for example, may mean that a patient is left without a functional joint for months, along with the requirement for two surgeries (i.e., infected implant removal and eventual replacement) (1). Intrathoracic device infections may require major surgical procedures, involving repeat sternotomy. The removal of some devices, such as vascular graft bypasses, may be impossible, rendering associated infection incurable with current approaches.The pathogenesis of device-associated infections relates to the presence of microorganisms in biofilms. Existence within a biofilm represents a survival strategy for microorganisms, protecting them from environmental influences, the host immune system, and, unfortunately, therapeutic levels of conventional antimicrobial agents. Biofilms exhibit ...

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Section: Discussionmentioning

confidence: 96%

Antibiofilm Activity of Low-Amperage Continuous and Intermittent Direct Electrical Current

Schmidt-Malan

Karau

Cede

et al. 2015

Antimicrob Agents Chemother

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“…After centrifugation at 15,000 rpm for 5 min, cell pellets were suspended and fixed in glutaraldehyde (3% aqueous solution). Other steps employed to prepare samples for SEM and TEM were carried out according to the methods described by Racyte and Zituni [ 1 , 40 ].…”

Section: Methodsmentioning

confidence: 99%

“…Alternating electric fields stall the planktonic growth of several prokaryotic microorganisms, such as Escherichia coli , Lactobacillus acidophilus, Staphylococcus aureus, Bacillus cereus, Pseudomonas putida, and P. fluorescens [ 1 – 6 ], leading to its recent application in food preservation [ 7 ]. The effectiveness of alternating electric field technology in preventing spoilage can be easily assessed by analyzing the amount of proteins eluted from cellular samples [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

“…The effectiveness of alternating electric field technology in preventing spoilage can be easily assessed by analyzing the amount of proteins eluted from cellular samples [ 7 ]. Alternating electric fields are generally used at either high intensity or high frequencies [ 1 , 3 , 4 , 7 , 8 ], and the possible applications of moderate electric fields (MEFs, typically <1–100 V/cm) at low frequencies (1–100 Hz) have been far less studied [ 9 ]. For example, planktonic L. acidophilus exhibits a decreased lag time following treatment with a MEF [ 6 , 9 ], causing a marked increase in bacteriocin yield [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

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Adaptation response of Pseudomonas fragi on refrigerated solid matrix to a moderate electric field

Chen

Zhang

et al. 2017

BMC Microbiol

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BackgroundModerate electric field (MEF) technology is a promising food preservation strategy since it relies on physical properties—rather than chemical additives—to preserve solid cellular foods during storage. However, the effectiveness of long-term MEF exposure on the psychrotrophic microorganisms responsible for the food spoilage at cool temperatures remains unclear.ResultsThe spoilage-associated psychrotroph Pseudomonas fragi MC16 was obtained from pork samples stored at 7 °C. Continuous MEF treatment attenuated growth and resulted in subsequent adaptation of M16 cultured on nutrient agar plates at 7 °C, compared to the control cultures, as determined by biomass analysis and plating procedures. Moreover, intracellular dehydrogenase activity and ATP levels also indicated an initial effect of MEF treatment followed by cellular recovery, and extracellular β-galactosidase activity assays indicated no obvious changes in cell membrane permeability. Furthermore, microscopic observations using scanning and transmission electron microscopy revealed that MEF induced sublethal cellular injury during early treatment stages, but no notable changes in morphology or cytology on subsequent days.ConclusionOur study provides direct evidence that psychrotrophic P. fragi MC16 cultured on nutrient agar plates at 7 °C are capable of adapting to MEF treatment.

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“…Nevertheless, the chemical in- Liu et al [14] established that the flow-through electrochemical technique with the CFF electrode may ensure the considerable reduction of Escherichia coli and low energy-consumption at the applied voltage under 3 V [14]. Nevertheless, there are numerous microbes in the water medium, comprising Gram-negative and Gram-positive bacteria, which differ mostly in the structure and composition of the cell membrane [27] [28], and possess various resistance to the disinfection technique [29]. Therefore, the dissimilarities of disinfection efficiency among Gram-negative and Gram-positive bacteria require to be more studied consistently.…”

Section: Introductionmentioning

confidence: 99%

Disinfecting Water with the Carbon Fiber-Based Flow-Through Electrode System (FES): Towards Axial Dispersion and Velocity Profile

Ghernaout¹,

Elboughdiri²,

Ghareba³

et al. 2020

OALib

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Alternating electric fields combined with activated carbon for disinfection of Gram negative and Gram positive bacteria in fluidized bed electrode system

Cited by 14 publications

References 37 publications

Antibiofilm Activity of Low-Amperage Continuous and Intermittent Direct Electrical Current

Antibiofilm Activity of Low-Amperage Continuous and Intermittent Direct Electrical Current

Adaptation response of Pseudomonas fragi on refrigerated solid matrix to a moderate electric field

Disinfecting Water with the Carbon Fiber-Based Flow-Through Electrode System (FES): Towards Axial Dispersion and Velocity Profile

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