Alternative culture medium design for biomass production of autochthonous meat starter Latilactobacillus sakei sp. sakei ACU-2

Abstract

The autochthonous strain Latilactobacillus sakei sp. sakei ACU-2 was selected as a meat starter culture for dry sausage production. Transferring this strain from laboratory scale to industry requires an increase in biomass production, while lowering process costs. In this study, a combination of techniques was applied in order to optimize the culture medium composition to enhance biomass production of L. sakei ACU-2. One variable at a time experiments, Plackett–Burman design, and mixture design were performed to fulfill the strain nutritional requirements. Eventually, the optimized formulation contained 19.46 g/L yeast extract; 8.28 g/L whey protein concentrate; 2.26 g/L soy peptone; 30 g/L cerelose; 1 g/L Tween 80; 5 g/L sodium acetate; 0.2 g/L magnesium sulfate and 0.05 g/L manganese sulfate. When L. sakei ACU-2 was cultivated in a bioreactor using the alternative medium, an enhancement of 75.5% of biomass production was achieved, in comparison to its growth in the commercial de Man, Rogosa, and Sharpe medium. Furthermore, a reduction of 62–86% of the cost was also attained. These results support a promising large-scale application of the designed medium for high biomass yields of the starter culture at minor costs.

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“…Differently from all others, in medium 2 the lower concentration of MnSO 4 combined with the absence of sodium acetate, that acts as buffering agent and growth cofactor, might explain the lower biomass yield obtained. These data are in agreement with those reported by Galante and collaborators, optimizing medium design for the growth of Latilactobacillus sakei by one variable at time experiments ( Galante et al, 2023 ). Ammonium citrate, was also present in all media tested besides medium 2; this salt is normally added to select for LAB strains and avoid growth of contaminants, and it was reported to promote growth of certain LAB ( Waśko et al, 2010 ; Papizadeh et al, 2020 ).…”