Alternative Evaluation to Earthworm Toxicity Test in Polychlorinated Biphenyls Spiked and Remediated Soils

Yu, Chungui; Zhang, Congkai; Yang, Zhe; Tang, Xianjin; Wan, Jixing; Shen, Chaofeng

doi:10.1007/s00128-020-02939-1

Cited by 1 publication

(1 citation statement)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is estimated that more than 1 million tons of PCBs have been produced and used, of which 1/4 to 1/3 has entered the environment. Although many developed countries have restricted the use of PCBs or substituted them by materials with lower toxicity, large amounts of discarded PCBs are still widely present in the environment ( 4 , 5 ). PCBs can be accumulated in environmental media and be biomagnified through the food chain because of their bioaccumulation potential ( 3 ).…”

Section: Introductionmentioning

confidence: 99%

Aroclor 1254 induced inhibitory effects on osteoblast differentiation in murine MC3T3-E1 cells through oxidative stress

Chen

Cai²,

Chen³

et al. 2022

Front. Endocrinol.

View full text Add to dashboard Cite

This study aimed to evaluate the osteotoxicity of polychlorinated biphenyls in murine osteoblastic MC3T3-E1 cells, and to explore the underlying mechanism focused on oxidative stress. The cells were exposed to Aroclor 1254 at concentrations of 2.5-20 µmol/L, and then cell viability, oxidative stress, intracellular calcium concentration, osteocalcin content, and calcium nodules formation were measured. Aroclor 1254 reduced cell viability and induced overproduction of intracellular reactive oxygen species in a dose-dependent manner. Activity of superoxide dismutase was decreased, and malondialdehyde content was promoted after exposure. Moreover, inhibitory effects of Aroclor 1254 on calcium metabolism and mineralization of osteoblasts were observed, as indicated by reduction of the intracellular calcium concentration, osteocalcin content, and modules formation rate. The decreased expression of osteocalcin, alkaline phosphatase, bone sialoprotein, and transient receptor potential vanilloid 6 further confirmed the impairment of Aroclor 1254 on calcium homeostasis and osteoblast differentiation. Addition of the antioxidant N-acetyl-L-cysteine partially restored the inhibitory effects on calcium metabolism and mineralization. In general, Aroclor 1254 exposure reduces calcium homeostasis, osteoblast differentiation and bone formation, and oxidative stress plays a vital role in the underlying molecular mechanism of osteotoxicity.

show abstract

Section: Introductionmentioning

confidence: 99%