Alternative splice variants and differential relative abundance patterns of vasa mRNAs during gonadal development in the Chinese mitten crab Eriocheir sinensis

Yang, Guocui; Wang, Ruirui; Liu, Zhiqiang; Knepper, M. A.; Feng, Jianbin; Qiu, Gao‐Feng

doi:10.1016/j.anireprosci.2019.106131

Cited by 5 publications

(3 citation statements)

References 31 publications

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“…Importantly, we also revealed the four isoform variants of Vasa, which may imply having different biology functions during sturgeon early gametogenesis. Recent evidences indicate that different splice variants of Vasa exist in many species, including bovine [86] and Chinese mitten crab [87]. The qPCR further analysis indicated that the splicing variants of Vasa had different relative proportions during bull spermatogenesis.…”

Section: Discussionmentioning

confidence: 92%

Full-length transcriptome sequencing and comparative transcriptomic analysis to uncover genes involved in early gametogenesis in the gonads of Amur sturgeon (Acipenser schrenckii)

Zhang¹,

Zhou²,

Li³

et al. 2020

Front Zool

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Full-length transcriptome sequencing and comparative transcriptomic analysis to uncover genes involved in early gametogenesis in the gonads of Amur sturgeon (Acipenser schrenckii) AbstractBackground: Sturgeons (Acipenseriformes) are polyploid chondrostean fish that constitute an important model species for studying development and evolution in vertebrates. To better understand the mechanisms of reproduction regulation in sturgeon, this study combined PacBio isoform sequencing (Iso-Seq) with Illumina short-read RNA-seq methods to discover full-length genes involved in early gametogenesis of the Amur sturgeon, Acipenser schrenckii.Results: A total of 50.04 G subread bases were generated from two SMRT cells, and herein 164,618 nonredundant full-length transcripts (unigenes) were produced with an average length of 2782 bp from gonad tissues (three testes and four ovaries) from seven 3-year-old A. schrenckii individuals. The number of ovary-specific expressed unigenes was greater than those of testis (19,716 vs. 3028), and completely different KEGG pathways were significantly enriched between the ovary-biased and testis-biased DEUs. Importantly, 60 early gametogenesisrelated genes (involving 755 unigenes) were successfully identified, and exactly 50% (30/60) genes of those showed significantly differential expression in testes and ovaries. Among these, the Amh and Gsdf with testisbiased expression, and the Foxl2 and Cyp19a with ovary-biased expression strongly suggested the important regulatory roles in spermatogenesis and oogenesis of A. schrenckii, respectively. We also found the four novel Sox9 transcript variants, which increase the numbers of regulatory genes and imply function complexity in early gametogenesis. Finally, a total of 236,672 AS events (involving 36,522 unigenes) were detected, and 10,556 putative long noncoding RNAs (lncRNAs) and 4339 predicted transcript factors (TFs) were also respectively identified, which were all significantly associated with the early gametogenesis of A. schrenckii.

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Section: Discussionmentioning

confidence: 92%

Full-length transcriptome sequencing and comparative transcriptomic analysis to uncover genes involved in early gametogenesis in the gonads of Amur sturgeon (Acipenser schrenckii)

Zhang¹,

Zhou²,

Li³

et al. 2020

Front Zool

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“…VASA has been regarded as a relatively specific marker of germ cells, especially postmeiotic germ cells (35). And VASA may regulate gonadal development by alternative splicing (36). Parte et al (37) showed expression characteristics of VASA during ovarian stem cells differentiation and found that VASA was expressed in the cytoplasm of progenitor ovarian stem cells.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The dynamic expression of SOX17 in germ cells from human female foetus and adult ovaries after specification

et al. 2023

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BackgroundSOX17 has been identified as a critical factor in specification of human primordial germ cells, but whether SOX17 regulates development of germ cells after sex differentiation is poorly understood. MethodsWe collected specimens of gonadal ridge from an embryo (n=1), and ovaries of foetuses (n=23) and adults (n=3). Germ cells were labelled with SOX17, VASA (classic germ cells marker), phosphohistone H3 (PHH3, mitosis marker) and synaptonemal complex protein 3 (SCP3, meiosis marker). ResultsSOX17 was detected in both cytoplasm and nucleus of oogonia and oocytes of primordial and primary follicles from 15 to 28 gestational weeks (GW). However, it was exclusively expressed in cytoplasm of oogonia at 7 GW, and in nucleus of oocytes in secondary follicles. Co-expression rates of SOX17 in VASA+ germ cells ranged from 81.29% to 97.81% in foetuses. Co-staining rates of SOX17 and PHH3 or SCP3 were 0%-34% and 0%-57%, respectively. Interestingly, we distinguished a subpopulation of SOX17+VASA- germ cells in fetal ovaries. These cells clustered in the cortex and could be co-stained with the mitosis marker PHH3 but not the meiosis marker SCP3.ConclusionsThe dynamic expression of SOX17 was detected in human female germ cells. We discovered a population of SOX17+ VASA- germ cells clustering at the cortex of ovaries. We could not find a relationship between mitosis or meiosis and SOX17 or VASA staining in germ cells. Our findings provide insight into the potential role of SOX17 involving germ cells maturation after specification, although the mechanism is unclear and needs further investigation.

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Seasonal composition of immature germ cells in the Yesso scallop identified by vasa-like gene (my-vlg) and protein expression, with evidence of irregular germ cell differentiation accompanied with a high mortality event

Mokrina

Nagasawa

Kanamori

et al. 2021

Aquaculture Reports

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Alternative splice variants and differential relative abundance patterns of vasa mRNAs during gonadal development in the Chinese mitten crab Eriocheir sinensis

Cited by 5 publications

References 31 publications

Full-length transcriptome sequencing and comparative transcriptomic analysis to uncover genes involved in early gametogenesis in the gonads of Amur sturgeon (Acipenser schrenckii)

Full-length transcriptome sequencing and comparative transcriptomic analysis to uncover genes involved in early gametogenesis in the gonads of Amur sturgeon (Acipenser schrenckii)

The dynamic expression of SOX17 in germ cells from human female foetus and adult ovaries after specification

Seasonal composition of immature germ cells in the Yesso scallop identified by vasa-like gene (my-vlg) and protein expression, with evidence of irregular germ cell differentiation accompanied with a high mortality event

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