Alternative splicing and immune response of Crassostrea gigas tumor necrosis factor receptor-associated factor 3

Huang, Baoyu; Zhang, Linlin; Du, Yishuai; Li, Li; Qu, Tao; Meng, Jie; Zhang, Guofan

doi:10.1007/s11033-014-3531-9

Cited by 20 publications

(9 citation statements)

References 41 publications

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“…A similar expression pattern of TRAF3 under bacterial invasion was also reported in Mediterranean mussel (M. galloprovincialis) (Toubiana et al, 2014) and pearl oyster (P. fucata) (Huang et al, 2012). Interestingly, there are two TRAF3 isoforms in C. gigas, which display contrary expression responses to Gram-negative bacterial challenge: CgTRAF3-L is significantly up-regulated, whereas CgTRAF3-S is significantly down-regulated (Huang et al, 2014). No such multiple isoforms of TRAF3 have yet been isolated in other bivalve species.…”

Section: Pytraf3ip1 Expression Was Ubiquitously Detected Insupporting

confidence: 74%

“…With innate immunity, they have evolved elaborate mechanisms to protect themselves against pathogenic invasion. As an important gene that functions in innate immunity, TRAF3 has been identified and characterized in several marine bivalves, such as Pinctada fucata (Huang et al, 2012), Crassostrea gigas (Huang et al, 2014), Patinopecten yessoensis and Mytilus galloprovincialis (Toubiana et al, 2014). The regulated expression of bivalve TRAF3s in response to bacterial challenge that has been revealed in these studies implies the involvement of TRAF3 and its related molecules in innate immunity of shellfish.…”

Section: Introductionmentioning

confidence: 65%

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Characterization of the TRAF3IP1 gene in Yesso scallop (Patinopecten yessoensis) and its expression in response to bacterial challenge

Cheng

Wang

et al. 2016

Genes Genet. Syst.

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Tumor necrosis factor receptor-associated factor 3 (TRAF3) is an important adaptor that transmits upstream activation signals to induce innate immune responses. TRAF3 interacting protein 1 (TRAF3IP1) interacts specifically with TRAF3, but its function in innate immunity remains unclear, especially in marine invertebrates. In this study, to better understand the functions of TRAFs in innate immune responses, we identified and characterized the first bivalve TRAF3IP1 gene, PyTRAF3IP1, from Yesso scallop (Patinopecten yessoensis), one of the most important mollusk species for aquaculture. The PyTRAF3IP1 cDNA is 2,367 bp, with an open reading frame of 1,629 bp encoding 542 amino acids. Phylogenetic and protein structural analysis confirmed the gene's identity and revealed that PyTRAF3IP1 was more similar to vertebrate TRAF3IP1s than to those of invertebrates. PyTRAF3IP1 was expressed in all the adult tissues and developmental stages sampled, implying that it plays versatile roles in many biological processes. Furthermore, PyTRAF3IP1 expression was dramatically induced in the acute phase (3-6 h) after infection with both Gram-positive (Micrococcus luteus) and Gram-negative (Vibrio anguillarum) bacteria, even stronger induction being observed after V. anguillarum challenge. This is the first report of the characterization and immune response involvement of TRAF3IP1 in marine invertebrates, and suggests that TRAF3IP1 contributes to innate immunity in bivalves.

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Section: Pytraf3ip1 Expression Was Ubiquitously Detected Insupporting

confidence: 74%

Section: Introductionmentioning

confidence: 65%

Characterization of the TRAF3IP1 gene in Yesso scallop (Patinopecten yessoensis) and its expression in response to bacterial challenge

Cheng

Wang

et al. 2016

Genes Genet. Syst.

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“…There is also evidence of massive expansion and functional divergence among oyster innate immunity genes 39 . To better understand the function of the oyster RLR signalling pathway, we first cloned a number of key oyster genes homologous to those encoding mammalian RLR antiviral signalling pathway proteins, including CgRIG-I-1 ( Cg24392 , GenBank Accession number: KY630188), CgMAVS (GenBank Accession number: KY630189), CgTRAF2 44 ( Cg08964 ), CgTRAF3 45 ( Cg23158 ), CgTRAF6 ( Cg10206 , GenBank Accession number: KY630190), and two oyster IRF genes, CgIRF2 ( Cg21171 , GenBank Accession number: KY630191) and CgIRF8 ( Cg03270 , GenBank Accession number: KY630192), (see Supplementary data for sequence details) from the genome of C. gigas . Importantly, this includes the first reported cloning of a MAVS gene from an invertebrate.…”

Section: Resultsmentioning

confidence: 99%

Characterization of the Mollusc RIG-I/MAVS Pathway Reveals an Archaic Antiviral Signalling Framework in Invertebrates

Huang

Zhang

et al. 2017

Sci Rep

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Despite the mitochondrial antiviral signalling protein (MAVS)-dependent RIG-I-like receptor (RLR) signalling pathway in the cytosol plays an indispensable role in the antiviral immunity of the host, surprising little is known in invertebrates. Here we characterized the major members of RLR pathway and investigated their signal transduction a Molluscs. We show that genes involved in RLR pathway were significantly induced during virus challenge, including CgRIG-I-1, CgMAVS, CgTRAF6 (TNF receptor-associated factor 6), and CgIRFs (interferon regulatory factors. Similar to human RIG-I, oyster RIG-I-1 could bind poly(I:C) directly in vitro and interact with oyster MAVS via its caspase activation and recruitment domains. We also show that transmembrane domain-dependent self-association of CgMAVS may be crucial for its signalling and that CgMAVS can recruit the downstream signalling molecule, TRAF6, which can subsequently activate NF-κB signal pathway. Moreover, oyster IRFs appeared to function downstream of CgMAVS and were able to activate the interferon β promoter and interferon stimulated response elements in mammalian cells. These results establish invertebrate MAVS-dependent RLR signalling for the first time and would be helpful for deciphering the antiviral mechanisms of invertebrates and understanding the development of the vertebrate RLR network.

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“…edulis , MAVS was also found in C . gigas , where its inhibition led to a decrease in TRAF3 expression [ 79 ]. The results of this recent study not only showed that MAVS exists in other oyster species aside from S .…”

Section: Resultsmentioning

confidence: 99%

Transcriptome Analysis of the Sydney Rock Oyster, Saccostrea glomerata: Insights into Molluscan Immunity

et al. 2016

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BackgroundOysters have important ecological functions in their natural environment, acting as global carbon sinks and improving water quality by removing excess nutrients from the water column. During their life-time oysters are exposed to a variety of pathogens that can cause severe mortality in a range of oyster species. Environmental stressors encountered in their habitat can increase the susceptibility of oysters to these pathogens and in general have been shown to impact on oyster immunity, making immune parameters expressed in these marine animals an important research topic.ResultsPaired-end Illumina high throughput sequencing of six S. glomerata tissues exposed to different environmental stressors resulted in a total of 484,121,702 paired-end reads. When reads and assembled transcripts were compared to the C. gigas genome, an overall low level of similarity at the nucleotide level, but a relatively high similarity at the protein level was observed. Examination of the tissue expression pattern showed that some transcripts coding for cathepsins, heat shock proteins and antioxidant proteins were exclusively expressed in the haemolymph of S. glomerata, suggesting a role in innate immunity. Furthermore, analysis of the S. glomerata ORFs showed a wide range of genes potentially involved in innate immunity, from pattern recognition receptors, components of the Toll-like signalling and apoptosis pathways to a complex antioxidant defence mechanism.ConclusionsThis is the first large scale RNA-Seq study carried out in S. glomerata, showing the complex network of innate immune components that exist in this species. The results confirmed that many of the innate immune system components observed in mammals are also conserved in oysters; however, some, such as the TLR adaptors MAL, TRIF and TRAM are either missing or have been modified significantly. The components identified in this study could help explain the oysters’ natural resilience against pathogenic microorganisms encountered in their natural environment.

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Alternative splicing and immune response of Crassostrea gigas tumor necrosis factor receptor-associated factor 3

Cited by 20 publications

References 41 publications

Characterization of the <i>TRAF3IP1</i> gene in Yesso scallop (<i>Patinopecten yessoensis</i>) and its expression in response to bacterial challenge

Characterization of the <i>TRAF3IP1</i> gene in Yesso scallop (<i>Patinopecten yessoensis</i>) and its expression in response to bacterial challenge

Characterization of the Mollusc RIG-I/MAVS Pathway Reveals an Archaic Antiviral Signalling Framework in Invertebrates

Transcriptome Analysis of the Sydney Rock Oyster, Saccostrea glomerata: Insights into Molluscan Immunity

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