Alternative splicing results in a lack of starch synthase IIa-D in Chinese wheat landrace

Zhou, Yanjie; Zhong, Xiao‐bo; Tang, Huaping; Deng, Mei; Ma, Jian; Qi, Pengfei; Wang, Jirui; Chen, Guoyue; Liu, Yaxi; Lu, Zhen-Xiang; Li, Wei; Lan, Xiujin; Wei, Yuming; Zheng, Youliang; Jiang, Qiantao

doi:10.1139/gen-2017-0246

Cited by 5 publications

(3 citation statements)

References 50 publications

(56 reference statements)

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“…The genome-wide AS profiling uncovered a very complex AS landscape in wheat, exhibiting that almost 22% of genes occur AS ( Yu et al., 2020 ). Increasing evidences have uncovered that AS events in wheat are enormously related to grain-related traits such as polyphenol oxidase activity ( Sun et al., 2010 ), starch synthase activity ( Zhou et al., 2018 ), and grain weight and size ( Ren et al., 2021 ). AS is also related to abiotic stresses tolerance ( Egawa et al., 2006 ; Liu et al., 2018 ) and disease resistance ( Wang et al., 2020 ; Sánchez-Martín et al., 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Two alternative splicing variants of a wheat gene TaNAK1, TaNAK1.1 and TaNAK1.2, differentially regulate flowering time and plant architecture leading to differences in seed yield of transgenic Arabidopsis

Zhang

et al. 2022

Front. Plant Sci.

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In wheat production, appropriate flowering time and ideal plant architecture are the prerequisites for high grain yield. Alternative splicing (AS) is a vital process that regulates gene expression at the post-transcriptional level, and AS events in wheat have been found to be closely related to grain-related traits and abiotic stress tolerance. However, AS events and their biological roles in regulating flowering time and plant architecture in wheat remain unclear. In this study, we report that TaNAK1 undergoes AS, producing three splicing variants. Molecular characterization of TaNAK1 and its splicing variants demonstrated that all three protein isoforms have a conserved NB-ARC domain and a protein kinase domain, but the positions of these two domains and the length of the protein kinase domains are different among them, implying that they may have different three-dimensional structures and therefore have different functions. Further investigations showed that the two splicing variants of TaNAK1, TaNAK1.1 and TaNAK1.2, exhibited different expression patterns during wheat growth and development, while the other one, TaNAK1.3, was not detected. Subcellular localization demonstrated that TaNAK1.1 was mainly localized in the cytoplasm, while TaNAK1.2 was localized in the nucleus and cytoplasm. Both TaNAK1.1 and TaNAK1.2 exhibit protein kinase activity in vitro. Ectopic expression of TaNAK1.1 and TaNAK1.2 in Arabidopsis demonstrated that these two splicing variants play opposite roles in regulating flowering time and plant architecture, resulting in different seed yields. TaNAK1.2 positive regulates the transition from vegetative to reproductive growth, plant height, branching number, seed size, and seed yield of Arabidopsis, while TaNAK1.1 negatively regulates these traits. Our findings provide new gene resource for regulating flowering time and plant architecture in crop breeding for high grain yield.

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Section: Introductionmentioning

confidence: 99%

Two alternative splicing variants of a wheat gene TaNAK1, TaNAK1.1 and TaNAK1.2, differentially regulate flowering time and plant architecture leading to differences in seed yield of transgenic Arabidopsis

Zhang

et al. 2022

Front. Plant Sci.

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“…For example, AS results in different splicing variants of ABI3 ( Sugliani, et al, 2010 ), thereby affecting the abscisic acid signaling pathway. In wheat, different variants of TaSSIIa-D produced by AS determine the straight-chain starch content in the seeds ( Zhou et al, 2018 ). The AS events associated with different developmental stages greatly affect the yield of rice ( Oryza sativa ) ( Xing and Zhang., 2010 ).…”

Section: Introductionmentioning

confidence: 99%

Analysis of Whole-Transcriptome RNA-Seq Data Reveals the Involvement of Alternative Splicing in the Drought Response of Glycyrrhiza uralensis

Huang

et al. 2022

Front. Genet.

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Alternative splicing (AS) is a post-transcriptional regulatory mechanism that increases protein diversity. There is growing evidence that AS plays an important role in regulating plant stress responses. However, the mechanism by which AS coordinates with transcriptional regulation to regulate the drought response in Glycyrrhiza uralensis remains unclear. In this study, we performed a genome-wide analysis of AS events in G. uralensis at different time points under drought stress using a high-throughput RNA sequencing approach. We detected 2,479 and 2,764 AS events in the aerial parts (AP) and underground parts (UP), respectively, of drought-stressed G. uralensis. Of these, last exon AS and exon skipping were the main types of AS. Overall, 2,653 genes undergoing significant AS regulation were identified from the AP and UP of G. uralensis exposed to drought for 2, 6, 12, and 24 h. Gene Ontology analyses indicated that AS plays an important role in the regulation of nitrogen and protein metabolism in the drought response of G. uralensis. Notably, the spliceosomal pathway and basal transcription factor pathway were significantly enriched with differentially spliced genes under drought stress. Genes related to splicing regulators in the AP and UP of G. uralensis responded to drought stress and underwent AS under drought conditions. In summary, our data suggest that drought-responsive AS directly and indirectly regulates the drought response of G. uralensis. Further in-depth studies on the functions and mechanisms of AS during abiotic stresses will provide new strategies for improving plant stress resistance.

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“…AS dynamics and evolutionary divergence during embryogenesis in wheat species indicated that AS provides opportunities for transcriptional and proteomic plasticity, and the potential for generating trait diversity [ 12 ]. Some reports have illustrated that AS events in wheat are substantially related to grain biochemical traits such as phytoene synthase activity [ 19 ], polyphenol oxidase activity [ 25 ], and starch synthase activity [ 26 ]. AS is also involved in disease resistance [ 27 ] and abiotic stresses tolerance [ 28 , 29 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

Alternative Splicing of TaGS3 Differentially Regulates Grain Weight and Size in Bread Wheat

Ren

Zhi

Liu

et al. 2021

IJMS

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The heterotrimeric G-protein mediates growth and development by perceiving and transmitting signals in multiple organisms. Alternative splicing (AS), a vital process for regulating gene expression at the post-transcriptional level, plays a significant role in plant adaptation and evolution. Here, we identified five splicing variants of Gγ subunit gene TaGS3 (TaGS3.1 to TaGS3.5), which showed expression divergence during wheat polyploidization, and differential function in grain weight and size determination. TaGS3.1 overexpression significantly reduced grain weight by 5.89% and grain length by 5.04%, while TaGS3.2–3.4 overexpression did not significantly alter grain size compared to wild type. Overexpressing TaGS3.5 significantly increased the grain weight by 5.70% and grain length by 4.30%. Biochemical assays revealed that TaGS3 isoforms (TaGS3.1–3.4) with an intact OSR domain interact with WGB1 to form active Gβγ heterodimers that further interact with WGA1 to form inactive Gαβγ heterotrimers. Truncated isoforms TaGS3.2–3.4 , which lack the C-terminal Cys-rich region but have enhanced binding affinity to WGB1, antagonistically compete with TaGS3.1 to bind WGB1, while TaGS3.5 with an incomplete OSR domain does not interact with WGB1. Taking these observations together, we proposed that TaGS3 differentially regulates grain size via AS, providing a strategy by which the grain size is fine-tuned and regulated at the post-transcriptional level.

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Alternative splicing results in a lack of starch synthase IIa-D in Chinese wheat landrace

Cited by 5 publications

References 50 publications

Two alternative splicing variants of a wheat gene TaNAK1, TaNAK1.1 and TaNAK1.2, differentially regulate flowering time and plant architecture leading to differences in seed yield of transgenic Arabidopsis

Two alternative splicing variants of a wheat gene TaNAK1, TaNAK1.1 and TaNAK1.2, differentially regulate flowering time and plant architecture leading to differences in seed yield of transgenic Arabidopsis

Analysis of Whole-Transcriptome RNA-Seq Data Reveals the Involvement of Alternative Splicing in the Drought Response of Glycyrrhiza uralensis

Alternative Splicing of TaGS3 Differentially Regulates Grain Weight and Size in Bread Wheat

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