Aluminosilicate Nanocomposite on Genosensor: A Prospective Voltammetry Platform for Epidermal Growth Factor Receptor Mutant Analysis in Non-small Cell Lung Cancer

Abstract

Lung cancer is one of the most serious threats to human where 85% of lethal death caused by non-small cell lung cancer (NSCLC) induced by epidermal growth factor receptor (EGFR) mutation. The present research focuses in the development of efficient and effortless EGFR mutant detection strategy through high-performance and sensitive genosensor. The current amplified through 250 µm sized fingers between 100 µm aluminium electrodes indicates the voltammetry signal generated by means of the mutant DNA sequence hybridization. To enhance the DNA immobilization and hybridization, ∼25 nm sized aluminosilicate nanocomposite synthesized from the disposed joss fly ash was deposited on the gaps between aluminium electrodes. The probe, mutant (complementary), and wild (single-base pair mismatch) targets were designed precisely from the genomic sequences denote the detection of EGFR mutation. Fourier-transform Infrared Spectroscopy analysis was performed at every step of surface functionalization evidences the relevant chemical bonding of biomolecules on the genosensor as duplex DNA with peak response at 1150 cm−1 to 1650 cm−1. Genosensor depicts a sensitive EGFR mutation as it is able to detect apparently at 100 aM mutant against 1 µM DNA probe. The insignificant voltammetry signal generated with wild type strand emphasizes the specificity of genosensor in the detection of single base pair mismatch. The inefficiency of genosensor in detecting EGFR mutation in the absence of aluminosilicate nanocomposite implies the insensitivity of genosensing DNA hybridization and accentuates the significance of aluminosilicate. Based on the slope of the calibration curve, the attained sensitivity of aluminosilicate modified genosensor was 3.02E-4 A M−1. The detection limit of genosensor computed based on 3σ calculation, relative to the change of current proportional to the logarithm of mutant concentration is at 100 aM.

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“…When the oligoaptamer was immobilized on the CDI-Cu NF, stretching of (PO 4 ) 3− (the sugar-phosphate backbone of DNA) was observed at 1082 and 1230 cm −1 35 , 36 . The hydrogen bonds of the DNA strands are notable from 3000 to 3500 cm −1 37 . Upon detection of target β-lactoglobulin, the peak of the amide is observed to be in the range of 1566–1742 cm −1 .…”

Alzheimer’s Disease Determination by a Dual Probe on Gold Nanourchins and Nanohorn Hybrids

“…First, the electrode pattern was designed by AutoCAD software. 29 , 30 The sensor surface was organized by two electrodes with multiple junction triangles at equal micro gap distances. The following steps were used to prepare the electrode.…”

Liquid Biopsy-Based Biosensors for MRD Detection and Treatment Monitoring in Non-Small Cell Lung Cancer (NSCLC)

“…The calculated LoD of the aluminosilicate modified genosensor is 100 aM. In addition, a voltammetry interdigitated electrode (IDE) was developed to sensing genomic DNA hybridisation using microscale aluminium electrodes and gaps [122].…”