Alveolar Bone Density Reduction in Rats Caused by Unilateral Nasal Obstruction

Wang, Xue; Cao, Yongge; Liu, Zhenhua; Wang, Zihan; Chu, Xiaoying; Wang, Lei; Hu, Xuanxuan; Zhao, Han; Diao, Zhanqiu; Peng, Fengting; Ye, Hui; Cao, Zhongcheng

doi:10.4274/balkanmedj.galenos.2019.2018.12.11

Cited by 6 publications

(11 citation statements)

References 23 publications

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“…It has been reported that nasal obstruction negatively affects alveolar bone. 12 , 13 However, considering that previous studies were conducted using neonatal rats, our study provides meaningful data on the effect of nasal obstruction in adolescents.…”

Section: Discussionmentioning

confidence: 93%

Nasal obstruction promotes alveolar bone destruction in the juvenile rat model

Kim¹,

Man²,

Jang³

et al. 2022

Journal of Dental Sciences

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Background/purpose Nasal obstruction leads to oral breathing and consequently hypoxia. The purpose of this study was to determine the influence of hypoxia on inflammatory response and the effect on alveolar bone development in a rat model in which mouth breathing was induced by nasal obstruction. Materials and methods Unilateral nasal obstruction was performed by injecting a Merocel sponge into the nasal cavity of 8-week-old Sprague Dawley (SD) rats. After 3 and 6 weeks of nasal obstruction, rats were sacrificed, the organs were weighed, and the changes in mandibular bone quality were examined by micro-computed tomography (μ-CT). The stereomicroscope was used for the morphological analysis of alveolar bone loss in response to nasal obstruction. Hematoxylin and Eosin (H&E) and immunohistochemical staining were employed to examine inflammation and bone remodeling induced by hypoxia. Results Nasal obstruction led to a delay in overall growth and organ development. The bone mineral density (BMD) and bone volume/total volume (BV/TV) of the mandible were reduced due to nasal obstruction, and the loss of the alveolar bone was confirmed morphologically. Our nasal obstruction method was observed to be successful in inducing hypoxia along with an increase in hypoxia-inducible factor 1-alpha (HIF-α). Oral hypoxia induced by nasal obstruction increased inflammatory response, and increased expression of receptor activator of nuclear factor kappa-Β ligand (RANKL) led to bone destruction. Conclusion This study demonstrated that nasal obstruction induced mouth breathing led to hypoxia in a rat model. Under hypoxic conditions, an increase in osteoclast differentiation induced by activation of the inflammatory pathway causes destructive changes in the alveolar bone.

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Section: Discussionmentioning

confidence: 93%

Nasal obstruction promotes alveolar bone destruction in the juvenile rat model

Kim¹,

Man²,

Jang³

et al. 2022

Journal of Dental Sciences

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“…In addition, the current basic research suggests that the OPG/RANKL ratio may be more meaningful than the OPG levels alone during osteoclast formation. [ 29 ]…”

Section: Discussionmentioning

confidence: 99%

Association of osteoporosis risk and polymorphisms of osteoprotegerin gene T950C in postmenopausal Chinese women: A PRISMA-compliant meta-analysis

Xia

Chen

2023

Medicine

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Background: This study aimed to assess the association between the T950C polymorphism and osteoporosis in postmenopausal Chinese women to further reduce the influence of different genetic backgrounds by meta-analysis and subgroup analysis. Methods: Through November 2022, a systematic online investigation was performed with the aid of the Cochrane Library, EMBASE, PubMed, Web of Science and the Chinese National Knowledge Infrastructure to find case-control studies looking into the correlation between the osteoprotegerin gene (OPG) T950C polymorphism and postmenopausal osteoporosis susceptibility. Results: This study included 6 studies with a total of 1669 postmenopausal osteoporosis cases and 2992 controls. In the recessive model, postmenopausal women with the CC genotype (mutant homozygote at the T950C locus) had a lower risk of osteoporosis, indicating that the CC genotype of OPG T950C might show a preventive effect on osteoporosis after menopause. In a stratified analysis by geographic area, the population from South China had a significantly higher risk under the dominant model [CC + TC (heterozygote at the T950C locus) vs TT (wild-type homozygotes at the T950C locus): odds ratio = 1.34, 95% confidence interval = 1.17–1.54, P < .01], while the population from South China had a significantly lower risk under the recessive model (CC vs TC + TT: odds ratio = 0.79, 95% confidence interval = 0.69–0.95, P = .02). Conclusions: Together, the OPG T950C polymorphism may be associated with osteoporosis risk in postmenopausal Chinese women, according to this meta-analysis. Because of the study’s limitations, more large-scale research is needed to corroborate these findings.

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“…These results indicate that hypoxia can lead to oxidative stress and apoptosis in periodontal tissue. After modeling UNO in rats, rats presented with oral respiratory symptoms [24][25][26]. Oral respiration is one of the main clinical manifestations in patients with OSAHS [1].…”

Section: Discussionmentioning

confidence: 99%

“…Model. A UNO rat model was established by cauterizing the external nostril [24][25][26]. Briefly, 8-day-old male Wistar rats (n = 30) were randomly divided into two groups: SHAM and UNO.…”

Section: Animals and Unilateral Nasal Obstruction (Uno)mentioning

confidence: 99%

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Effects of Hypoxic Environment on Periodontal Tissue through the ROS/TXNIP/NLRP3 Inflammasome Pathway

Zhu

2022

BioMed Research International

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There is low evidence for the possible association between obstructive sleep apnea-hypopnea syndrome (OSAHS) and periodontitis, necessitating further research. This study was aimed at investigating this association. For the in vitro study, 8-day-old Wistar rats were divided into the unilateral nasal obstruction group (UNO) and the sham surgery group (SHAM). Rats in the former group were subjected to UNO by cauterization of the external nostril at the age of 8 days. Immunofluorescence analysis, quantitative real-time polymerase chain reaction, and western blot were performed to assess the expression of thioredoxin-interacting protein (TXNIP), NLR family pyrin domain-containing 3 (NLRP3) inflammasome-associated factors, and interleukin-1β (IL-1β). Throughout the experimental period, the weights of rats in the two groups were similar. The mRNA and protein expression of TXNIP and IL-1β was significantly higher in the UNO than in the SHAM groups. Compared with SHAM, NLRP3 inflammasome-associated factors were activated in the UNO group. For the in vitro study, a cellular hypoxia model was established by treating human periodontal ligament cells (HPDLCs) with cobalt chloride. The studies showed that hypoxia can induce an excessive production and accumulation of reactive oxygen species (ROS) in HPDLCs and induce abnormal expression of TNXIP, NLRP3 inflammasome-related factors, and IL-1β. More importantly, N-acetylcysteine induced reduction of ROS in HPDLCs, downregulated TXNIP expression, inhibited the expression and aggregation of NLRP3 inflammasome-related factors, and abrogated the inflammatory response to hypoxia. In conclusion, hypoxia-induced ROS can activate the TXNIP/NLRP3 inflammasome signaling pathway in response to oxidative stress, resulting in the increased expression of inflammatory factors in HPDLCs. Our findings provide evidence for the mechanism underlying the possible association between OSAHS and periodontal disease.

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Alveolar Bone Density Reduction in Rats Caused by Unilateral Nasal Obstruction

Cited by 6 publications

References 23 publications

Nasal obstruction promotes alveolar bone destruction in the juvenile rat model

Nasal obstruction promotes alveolar bone destruction in the juvenile rat model

Association of osteoporosis risk and polymorphisms of osteoprotegerin gene T950C in postmenopausal Chinese women: A PRISMA-compliant meta-analysis

Effects of Hypoxic Environment on Periodontal Tissue through the ROS/TXNIP/NLRP3 Inflammasome Pathway

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