Alveolar macrophage functions during the transition phase to active immunity in calves

Bertagnon, Heloisa Godoi; Batista, Camila Freitas; Santos, Karina Cecília Panelli; Gomes, Renata Nascimento; Bellinazzi, Jéssyca Beraldi; Libera, Alice Maria Melville Paiva Della

doi:10.1093/jas/sky460

Cited by 2 publications

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“…CD14 is one of the well-known lipopolysaccharide (LPS) receptors and is highly expressed on macrophages and monocytes [ 17 ]. Previous studies on bovine BALF have used CD14 expression to define alveolar macrophages and assess functional maturation [ 4 , 6 ]. However, human and experimental animal studies have shown that phenotypic markers of alveolar macrophages are very different from those of monocytes and other macrophages, and the expression of CD14 is known to be low as in our present report [ 5 , 19 ].…”

Section: Discussionmentioning

confidence: 99%

“…Since this study was conducted only on calves (15–60-days old), a further study is needed to determine whether the present method can be directly applied to adult cattle and other breeds. A report suggests that the maturation of bovine alveolar macrophages is not completed until the age of 6 months [ 6 ], and hence, a longer-term study will be necessary.…”

Section: Discussionmentioning

confidence: 99%

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Flow cytometric analysis of bronchoalveolar lavage fluid immune dynamics in calves

Ishikawa

Miyazawa

ZIBIKI

et al. 2022

The Journal of Veterinary Medical Science

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Understanding the immune dynamics in the respiratory mucosa of calves is necessary for a good management of bovine respiratory disease. Immune dynamics in the respiratory mucosa in humans and experimental animals has been assessed by flow cytometric analysis of bronchoalveolar lavage fluid (BALF); however, few reports have addressed this subject in calves. The aim of this study was to establish a universal method to analyze bronchoalveolar lavage fluid (BALF) by flow cytometry and to obtain basic knowledge of bovine respiratory mucosal immune dynamics. We investigated the immune cell populations in BALF and evaluated the surface antigen expression of alveolar macrophages in calves using flow cytometer. To further analyze the surface antigen variation observed in alveolar macrophages in detail, stimulation assays were performed in vitro. BALF cells were separated into three distinct populations based on their light scatter plot, which were considered to be macrophages, lymphocytes, and neutrophils. In most individuals, most of the BALF immune cells were alveolar macrophages, but an increased proportion of lymphocytes and neutrophils was observed in some individuals.Analysis of each surface antigen expression in alveolar macrophages showed that CD21 and MHC class II expression changed in response to changes in the leukocyte population.Moreover, when alveolar macrophages were stimulated with interferon-γ in vitro, the 3 expression of CD21 was drastically reduced and MHC class II was increased, suggesting that functional changes in alveolar macrophages themselves are involved in the immune dynamics.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Flow cytometric analysis of bronchoalveolar lavage fluid immune dynamics in calves

Ishikawa

Miyazawa

ZIBIKI

et al. 2022

The Journal of Veterinary Medical Science

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show abstract

Interferon gamma, lipopolysaccharide, and modified-live viral vaccines stimulation alter the mRNA expression of tumor necrosis factor α, inducible nitric oxide synthase, and interferon β in bovine alveolar macrophages

Ishikawa

Miyazawa

Tanaka

et al. 2022

Veterinary Immunology and Immunopathology

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Alveolar macrophage functions during the transition phase to active immunity in calves

Abstract: The article "Alveolar macrophage functions during the transition phase to active immunity in calves" by Bertagnon et al [doi: 10.1093/jas/ sky261] contained incorrect funding information, which has now been corrected.

Cited by 2 publications

References 0 publications

Flow cytometric analysis of bronchoalveolar lavage fluid immune dynamics in calves

Flow cytometric analysis of bronchoalveolar lavage fluid immune dynamics in calves

Interferon gamma, lipopolysaccharide, and modified-live viral vaccines stimulation alter the mRNA expression of tumor necrosis factor α, inducible nitric oxide synthase, and interferon β in bovine alveolar macrophages

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