1994
DOI: 10.19182/remvt.9138
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Amélioration de la technique de dissection du tractus digestif et des glandes salivaires des glossines pour la mise en évidence des divers stades de développement des trypanosomes

Abstract: Les auteurs décrivent une technique améliorée de dissection de l'intestin moyen et des glandes salivaires des glossines. En ajoutant à la dissection le proventricule, la méthode permet d'obtenir un tableau plus complet du développement des trypanosomes chez la mouche infectée.

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Cited by 5 publications
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“…The proboscis, midgut, and salivary glands were isolated for trypanosome detection using an optical microscope (X400). If trypanosomes were observed in at least one of the three organs, the organs were individually collected in Eppendorf ® tubes containing 25 μL of distilled water and stored at −20 °C for subsequent trypanosome species identification using polymerase chain reaction (PCR) [ 32 , 59 ].…”
Section: Methodsmentioning
confidence: 99%
“…The proboscis, midgut, and salivary glands were isolated for trypanosome detection using an optical microscope (X400). If trypanosomes were observed in at least one of the three organs, the organs were individually collected in Eppendorf ® tubes containing 25 μL of distilled water and stored at −20 °C for subsequent trypanosome species identification using polymerase chain reaction (PCR) [ 32 , 59 ].…”
Section: Methodsmentioning
confidence: 99%
“…La technique de dissection est celle décrite par Kazadi et coll. (8). L'observation microscopique a porté sur la présence ou l'absence de trypanosomes dans l'intestin moyen, le proventricule, le canal du jabot et le proboscis.…”
Section: Suivi Biologique Et Dissectionunclassified