Ameliorative Effect of Chitosan Complex on Miniature Pig Sperm Cryopreservation

Hong, Hye-Min; Sim, Ga-Young; Park, So-Mi; Lee, Eunjoo; Kim, Dae-Young

doi:10.12750/jet.2018.33.4.337

Cited by 3 publications

(2 citation statements)

References 15 publications

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“…The increasing socio-economic value of dogs has resulted in an increasing demand for advances in assisted reproductive technologies (ART) [2], particularly artificial insemination using cryopreserved semen [3]. Semen cryopreservation permits the exchange of genetic material among populations and preserves the genes of valuable individuals over extended periods [4,5]. In dogs, semen cryopreservation can help to conserve threatened breeds, enhance the use of certain genetic traits [6], and facilitate the breeding of individuals that are unable to breed naturally [7].…”

Section: Introductionmentioning

confidence: 99%

Myoinositol Supplementation of Freezing Medium Improves the Quality-Related Parameters of Dog Sperm

Qamar

Fang

Kim

et al. 2019

Animals

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Oxidative stress during freeze–thaw procedures results in reduced semen fertility. A decrease in free radical levels can improve the post-thaw sperm quality. We examined the effects of myoinositol supplementation in freezing medium on the structure and function of cryopreserved dog sperm. Pooled ejaculates were diluted with buffer without or with myoinositol (1 or 2 mg/mL). Analysis of fresh semen revealed that the optimal concentration of myoinositol was 1 mg/mL, and this concentration was used in further experiments. Post-thaw semen quality in the myoinositol-supplemented group was superior (p < 0.05) compared with that in the control group in terms of motility (57.9 ± 0.4% vs. 47.8 ± 0.2%), sperm viability (57.5 ± 0.5% vs. 44.6 ± 0.6%), intact plasma membrane (56.6 ± 0.4% vs. 46.2 ± 0.6%), and acrosome membrane (59.3 ± 0.5% vs. 51.8 ± 0.5%). In addition, sperm in the myoinositol-supplemented group showed a significantly lower expression of pro-apoptotic (BAX) and mitochondrial reactive oxygen species (ROS) modulator (ROMO1) genes but higher expression of anti-apoptotic (BCL2), and protamine-related (PRM2 and PRM3) genes compared with that in the control group. Therefore, myoinositol supplementation before freezing can protect against oxidative stress and improve post-thaw dog sperm quality.

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Section: Introductionmentioning

confidence: 99%

Myoinositol Supplementation of Freezing Medium Improves the Quality-Related Parameters of Dog Sperm

Qamar

Fang

Kim

et al. 2019

Animals

View full text Add to dashboard Cite

show abstract

“…These changes cause lower post-thaw sperm motility, decrease the integrity of the plasma and acrosomal membrane [7], and damage DNA, ultimately decreasing the fertilizing capability of sperm [8]. The factors responsible for reduced fertility of post-thaw sperm include ice formation, high osmotic pressure [9], reactive oxygen species (ROS) generation [6,10,11], and apoptotic pathway activation [12].…”

Section: Introductionmentioning

confidence: 99%

Improved Post-Thaw Quality of Canine Semen after Treatment with Exosomes from Conditioned Medium of Adipose-Derived Mesenchymal Stem Cells

Qamar

Fang

Kim

et al. 2019

Animals

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Simple SummaryIn this study, we have tried to exploit the potential role of exosomes-derived from canine adipose-derived mesenchymal stem cells in the protection and repair of damaged sperm during cryopreservation. The treatment of sperm with an optimal level of exosomes resulted in improved post-thaw semen quality. This improvement was due to the exosomal proteins that protect the sperm against oxidative damage and induce the repair of membranes and chromatin. The effect of exosomal proteins was also confirmed by higher expression of genes related to the repair of membranes and chromatin accompanied by the lower expression of a gene associated with reactive oxygen species production.AbstractFreezing decreases sperm quality, ultimately affecting fertilizing ability. The repair of freeze-damaged sperm is considered crucial for improving post-thaw viability and fertility. We investigated the effects of exosomes derived from canine adipose-derived mesenchymal stem cells on dog sperm structure and function during cryopreservation. The pooled ejaculate was diluted with buffer, without (Control), or with exosomal proteins (25, 50, or 100 µg/mL). Using fresh semen, the determined optimal exosomal protein concentration was 50 µg/mL (Group 2) which was used in further experiments. Post-thaw sperm treated with exosomes were superior to control (p < 0.05) in terms of motility (56.8 ± 0.3% vs. 47.2 ± 0.3%), live sperm percentage (55.9 ± 0.4% vs. 45.4 ± 0.4%), membrane integrity (55.6 ± 0.5% vs. 47.8 ± 0.3%), and acrosome integrity (60.4 ± 1.1% vs. 48.6 ± 0.4%). Moreover, expression of genes related to the repair of the plasma membrane (ANX 1, FN 1, and DYSF), and chromatin material (H3, and HMGB 1) was statistically higher in exosome-treated sperm than control, but the expression of the mitochondrial reactive oxygen species modulator 1 gene was significantly higher in control. Therefore, exosomal treatment may improve the quality of post-thaw dog semen through initiating damaged sperm repair and decreasing reactive oxygen species production.

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Chitosan as a Tool for Sustainable Development: A Mini Review

et al. 2022

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New developments require innovative ecofriendly materials defined by their biocompatibility, biodegradability, and versatility. For that reason, the scientific society is focused on biopolymers such as chitosan, which is the second most abundant in the world after cellulose. These new materials should show good properties in terms of sustainability, circularity, and energy consumption during industrial applications. The idea is to replace traditional raw materials with new ecofriendly materials which contribute to keeping a high production rate but also reducing its environmental impact and the costs. The chitosan shows interesting and unique properties, thus it can be used for different purposes which contributes to the design and development of sustainable novel materials. This helps in promoting sustainability through the use of chitosan and diverse materials based on it. For example, it is a good sustainable alternative for food packaging or it can be used for sustainable agriculture. The chitosan can also reduce the pollution of other industrial processes such as paper production. This mini review collects some of the most important advances for the sustainable use of chitosan for promoting circular economy. Hence, the present review focuses on different aspects of chitosan from its synthesis to multiple applications.

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Ameliorative Effect of Chitosan Complex on Miniature Pig Sperm Cryopreservation

Cited by 3 publications

References 15 publications

Myoinositol Supplementation of Freezing Medium Improves the Quality-Related Parameters of Dog Sperm

Myoinositol Supplementation of Freezing Medium Improves the Quality-Related Parameters of Dog Sperm

Improved Post-Thaw Quality of Canine Semen after Treatment with Exosomes from Conditioned Medium of Adipose-Derived Mesenchymal Stem Cells

Chitosan as a Tool for Sustainable Development: A Mini Review

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