Ameliorative Effect of Ginger Extract and Selenium in theThyroid Gland Toxicity Induced by Chlorpyrifos in Male Albino Rats

Abstract

Background: Chlorpyrifos CPF is one of the organophosphate insecticides that were interfered with the body's hormones. Thyroid hormones are necessary to maintained normal metabolism, growth and development and the disturbance of their levels leads to adverse medical conditions. Ginger extract and selenium are effective antioxidants. Aim of the work: This study examined effect of CPF on the thyroid gland structure and function and the ameliorative effect of ginger extract and selenium. Material and Methods: forty rats were categorized into four groups. Control group I, group II: rats were given CPF at a dose of 6.7 mg/kg orally five days / week for six weeks, group III: rats were given CPF and ginger extract at a dose of 750 mg/kg orally, five days / week for six weeks. Group IV: rats were given CPF and sodium selenite 10 µg/Kg orally five days / week for six weeks, blood samples were taken for hormonal essay. Thyroid gland specimens were prepared for the histological and immunohistochemical studies. Results: chlorpyrifos induced functional toxic effect and destruction of the thyroid gland histological structures. There was a significant decrease in area % of the colloid and a significant increase in mean number of PCNA positive nuclei in comparison with the control group. Ginger extract and selenium eliminated the damage effect of CPF on the thyroid gland function and histological structure. Conclusion: CPF had adverse effects on the thyroid structure and function that could be eliminated by administration of ginger and selenium.

Role of Histological Investigation in The Protective Evaluation of Ginger

“…Rats administrated Chlorpyrifos (organophosphate insecticide) and ginger extract at a dose of 750 mg/kg b.w. five days/week for six weeks showed some follicles of the thyroid gland with strong PAS reaction and others with negative PAS reaction (32) .…”

Ameliorative Impacts of Dactyloctenium aegyptium and Parapholis incurva Ethanolic Extracts on Sodium Fluoride Induced Pathological Alterations in the Thyroid Gland in Male Rats

“…12 (2022) results confirmed the present configuration of NaFinduced histologial alterations in thyroid follicles that revealed congestion of blood vessels, hemorrhage, inflammation and decrease in thyroid follicle size. El-Kerdasy et al [55] found thyroid follicle destruction with macrophages infiltration and a significant increase in serum TSH levels, as well as that chlorpyrifos toxic effects on thyroid gland tissue which may be due to lipid peroxidation associated with stellate cell stimulation for collagen synthesis in between thyroid follicles. Abdelaleem et al [8] reported that NaF causes major pathological changes in follicular cells and cellular components, resulting in decreased colloid secretion, as well as that the early reaction of TSH triggered thyroid follicular cells is ingestion of colloid material, which explains decreased luminal colloid.…”